Strain Name:

B6.129P2-Sncgtm1Vlb/J

Stock Number:

008843

Availability:

Under Development for Distribution Colony

To register your interest in this strain go to the Strain Interest Form.
These mice harbor a null mutation of the γ-synuclein gene (Sncg) and may be useful in studying synuclein function in neurodegenerative diseases, such as Parkinson's disease.

Description

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Vladimir Buchman,   Cardiff University School of Biosciences

Description
Mice homozygous for this γ-synuclein mutant allele are viable and fertile with no obvious abnormalities in development, behavior, or gross morphology of the nervous system. No RNA or protein expression from the targeted gene is observed. Slight upregulation of β-synuclein is reported in the midbrain of homozygous mice. Homozygous mice are also resistant to the inflammatory/apoptotic Parkinson's disease-like pathology that results following MPTP neurotoxin administration (the active form of which (MPP+) gains entry into dopaminergic cells via the dopamine transporter (DAT)). These γ-synuclein mutant mice may be useful in studying synuclein function in neurodegenerative diseases, such as Parkinson's disease.

Development
A targeting vector was designed to replace exons 1-3 of the targeted gene with a PGK-neo cassette. The construct was electroporated into 129P2/OlaHsd derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and the resulting chimeric animals were bred with C57BL/6J inbred mice. Next, mutant mice were backcrossed to C57BL/6J for at least 12 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) to establish the colony.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Sncgtm1Vlb/Sncgtm1Vlb

        B6.129P2-Sncgtm1Vlb
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:86439)
    • mice were viable, fertile, and showed no morphological or functional abnormalities of the nervous system
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Neurodegeneration
Parkinson's Disease
      resistance to MPTP
      synuclein mutants

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Sncgtm1Vlb
Allele Name targeted mutation 1, Vladimir L Buchman
Allele Type Targeted (knock-out)
Common Name(s) TgHSNCGtm1VLB;
Mutation Made By Vladimir Buchman,   Cardiff University School of Biosciences
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Sncg, synuclein, gamma
Chromosome 14
Gene Common Name(s) BCSG1; C79089; EST C79089; SR; persyn;
Molecular Note Exons 1 through 3 were replaced with a neomycin selection cassette inserted by homologous recombination. Transcript was undetected in homozygous mutant mice by Northern blot analysis of RNA obtained from various tissues. Western blot analysis of total spinal cord protein indicated an absence of protein in homozygous mutant mice. [MGI Ref ID J:86439]

Genotyping

Genotyping Information

Genotyping Protocols

Sncgtm1Vlb, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Ninkina N; Papachroni K; Robertson DC; Schmidt O; Delaney L; O'Neill F; Court F; Rosenthal A; Fleetwood-Walker SM; Davies AM; Buchman VL. 2003. Neurons expressing the highest levels of gamma-synuclein are unaffected by targeted inactivation of the gene. Mol Cell Biol 23(22):8233-45. [PubMed: 14585981]  [MGI Ref ID J:86439]

Senior SL; Ninkina N; Deacon R; Bannerman D; Buchman VL; Cragg SJ; Wade-Martins R. 2008. Increased striatal dopamine release and hyperdopaminergic-like behaviour in mice lacking both alpha-synuclein and gamma-synuclein. Eur J Neurosci 27(4):947-57. [PubMed: 18333965]  [MGI Ref ID J:132938]

Additional References

Sncgtm1Vlb related

Papachroni K; Ninkina N; Wanless J; Kalofoutis AT; Gnuchev NV; Buchman VL. 2005. Peripheral sensory neurons survive in the absence of alpha- and gamma-synucleins. J Mol Neurosci 25(2):157-64. [PubMed: 15784963]  [MGI Ref ID J:121324]

Robertson DC; Schmidt O; Ninkina N; Jones PA; Sharkey J; Buchman VL. 2004. Developmental loss and resistance to MPTP toxicity of dopaminergic neurones in substantia nigra pars compacta of gamma-synuclein, alpha-synuclein and double alpha/gamma-synuclein null mutant mice. J Neurochem 89(5):1126-36. [PubMed: 15147505]  [MGI Ref ID J:92213]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

 

This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development and On Hold

Supply Details

Standard SupplyUnder Development for Distribution Colony
Supply Notes

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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