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| These Ctgf-Tg2-Cre mice express Cre recombinase under the control of the Ctgf (connective tissue growth factor) promoter/enhancer regions, and may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex and hippocampus). | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 09-JUN-09 Species laboratory mouse Generation F?+ (08-JUN-09) Donating Investigator Ed Lein, Allen Institute for Brain Science (AIBS) Donating Investigator Theresa Zwingman, Allen Institute for Brain Science (AIBS) Description
Hemizygous Ctgf-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Ctgf-Tg2-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex and hippocampus).For characterization information, see images at the Allen Institute for Brain Science website (Ctgf-Tg2-Cre images (1), Ctgf-Tg2-Cre images (2), and Ctgf-Tg2-Cre images (3)).
Development
The 189 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-257E12, containing the entire Ctgf locus (and other genes), was modified by targeting a Cre recombinase coding sequence (the CreERT2 sequence modified back to encode the normal Cre recombinase gene), SV40 polyA signal, and frt-flanked neomycin cassette to the ATG start site of the Ctgf locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into embryos from B6C3 mice ((C57BL/6 x C3H)F1 x C57BL/6). Transgenic mice from founder line Ctgf-Tg2-Cre were subsequently backcrossed to C57BL/6J for two generations prior to arrival at The Jackson Laboratory. Upon arrival, transgenic mice were bred with C57BL/6J inbred mice for at least one additional generation to establish this colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100010 B6C3F1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Allen Institute for Brain Science cre strains
009616 B6.Cg-Tg(A930038C07Rik-cre)4Aibs/J 009613 B6.Cg-Tg(Scnn1a-cre)3Aibs/J 009614 B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J 008839 B6;C3-Tg(Cyp39a1-cre)1Aibs/J 009117 B6;C3-Tg(Cyp39a1-cre)7Aibs/J 008848 B6;C3-Tg(Mybpc1-cre)2Aibs/J 009111 B6;C3-Tg(Scnn1a-cre)1Aibs/J 009112 B6;C3-Tg(Scnn1a-cre)2Aibs/J 009103 B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J 009615 STOCK Tg(Cartpt-cre)1Aibs/J View Allen Institute for Brain Science cre strains (10 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Ctgf-cre)2Aibs | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Ed Lein | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Ctgf-Tg2-Cre; | ||
| Mutation Made By | Ed Lein, Allen Institute for Brain Science (AIBS) | ||
| Strain of Origin | (C57BL/6 x C3H)F1 X C57BL/6 | ||
| Site of Expression | Hemizygous Ctgf-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ctgf, connective tissue growth factor, mouse, laboratory | ||
| Driver Note | Ctgf | ||
| Molecular Note | The 189 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-257E12, containing the entire Ctgf locus (and other genes), was modified by targeting a Cre recombinase coding sequence (the CreERT2 sequence modified back to encode the normal Cre recombinase gene), SV40 polyA signal, and frt-flanked neomycin cassette to the ATG start site of the Ctgf locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. Cre expression is directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. [MGI Ref ID J:146821] | ||
| Gene Symbol and Name | Tg(Ctgf-cre)2Aibs, transgene insertion 2, Ed Lein | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Ctgf-Tg2-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Allen Institute for Brain Science. 2009. Cre transgenes created at the Allen Insitute for Brain Science (AIBS) (http://transgenicmouse.alleninstitute.org/imageseries/list/1.html?gene term=) MGI Direct Data Submission :. [MGI Ref ID J:146821]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (noncarrier) siblings, or to C57BL/6J inbred mice (Stock No. 000664). Mating System Noncarrier x Hemizygote (Female x Male) 09-JUN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Ctgf-cre)2Aibs
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Ctgf-cre)2Aibs x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Ctgf-cre)2Aibs
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Ctgf-cre)2Aibs
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Ctgf-cre)2Aibs x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Ctgf-cre)2Aibs
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100010 B6C3F1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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