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| These En2-cre line 22 transgenic mice (also called En2cre-22, En2cre line Tg22, or En2-cre tg-22) have the mouse engrailed 2 (En2) promoter/enhancer elements directing expression of Cre recombinase to a subset of En2-expressing cells; including the embryonic midbrain-hindbrain (mesencephalon-metencephalon) constriction region of the developing brain that gives rise to the posterior midbrain and medial cerebellum. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?pN1
Generation DefinitionsDonating Investigator Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center Description
En2-cre line 22 transgenic mice (also called En2cre-22, En2cre line Tg22, or En2-cre tg-22) are viable and fertile, with expression of Cre recombinase directed to the embryonic midbrain-hindbrain constriction region by mouse engrailed 2 promoter/enhancer elements. Specifically, whole-mount RNA in situ hybridization with a Cre recombinase antisense probe shows cre expression in a narrow dorsal domain surrounding the midbrain-hindbrain junction at embryonic day 9.5 (E9.5). When these En2-cre line 22 transgenic mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in a subset of En2-expressing tissues of the offspring; including the embryonic midbrain-hindbrain (mesencephalon-metencephalon) constriction region of the developing brain that gives rise to the posterior midbrain and medial cerebellum.When the donating investigator bred En2-cre line 22 transgenic mice with lacZ reporter mice, adult brain regions in the double transgenic offspring showed evidence of embryonic Cre recombinase activity; these include a) part of the midbrain structure responsible for visual processing (superior colliculus), much of the region responsible for auditory processing (inferior colliculus), and some regions responsible for homeostatic and reflexive pathways (tegmentum); b) some anterior hindbrain structures responsible for integration of sensory perception, coordination and motor control (primarily the medial cerebellum); and c) forebrain structures responsible for short term memory and spatial navigation (hippocampus).
Development
The En2-cre transgene was designed with the mouse engrailed 2 enhancer/promoter sequences (2.6 kbp enhancer fragment and 2.3 kbp minimal promoter sequences) upstream of a Cre recombinase cDNA sequence and followed by a metallothionein polyadenylation signal. This transgene was injected into CD1 zygotes. Founder mice were bred to CD1 outbred mice to establish founder line 22. These En2-cre line 22 transgenic mice (also called En2cre-22, En2cre line Tg22, or En2-cre tg-22) were bred to Swiss Webster outbred mice for many generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, En2cre-22 transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony at The Jackson Laboratory Repository.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of En2
002657 B6;129S2-En2tm1Alj/J 007924 STOCK En2tm4(cre/ERT2)Alj/J 007925 STOCK En2tm5.1Alj/J 008872 STOCK En2tm6Alj/J View Strains carrying other alleles of En2 (4 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
transplantation marker for embryonic and adult tissue
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(En2-cre)22Alj | ||
|---|---|---|---|
| Allele Name | transgene insertion 22, Alexandra L Joyner | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | En2-cre; | ||
| Mutation Made By | Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | The En2 promoter directs expression in the embryonic midbrain-hindbrain constriction region, the cerebellum, tectum, hippocampus and tegmentum. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | En2, engrailed 2, mouse, laboratory | ||
| Gene Symbol and Name | Tg(En2-cre)22Alj, transgene insertion 22, Alexandra L Joyner | ||
| Chromosome | UN | ||
| Gene Common Name(s) | En2-cre; | ||
| Driver Note | En2 | ||
| Molecular Note | The transgenic constuct contained a cre recombinase gene adjoined to an En2 minimal promoter and enhancer. The En2 promoter directs expression in the embryonic midbrain-hindbrain constriction region, the cerebellum, tectum, hippocampus and tegmentum. [MGI Ref ID J:67904] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Sgaier SK; Millet S; Villanueva MP; Berenshteyn F; Song C; Joyner AL. 2005. Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping. Neuron 45(1):27-40. [PubMed: 15629700] [MGI Ref ID J:95823]
Zinyk DL; Mercer EH; Harris E; Anderson DJ; Joyner AL. 1998. Fate mapping of the mouse midbrain-hindbrain constriction using a site-specific recombination system. Curr Biol 8(11):665-8. [PubMed: 9635195] [MGI Ref ID J:67904]
Additional References
Tg(En2-cre)22Alj relatedJandke A; Da Costa C; Sancho R; Nye E; Spencer-Dene B; Behrens A. 2011. The F-box protein Fbw7 is required for cerebellar development. Dev Biol 358(1):201-12. [PubMed: 21827743] [MGI Ref ID J:176598]
Marino S; Hoogervoorst D; Brandner S; Berns A. 2003. Rb and p107 are required for normal cerebellar development and granule cell survival but not for Purkinje cell persistence. Development 130(15):3359-68. [PubMed: 12810584] [MGI Ref ID J:83783]
Marino S; Krimpenfort P; Leung C; van der Korput HA; Trapman J; Camenisch I; Berns A; Brandner S. 2002. PTEN is essential for cell migration but not for fate determination and tumourigenesis in the cerebellum. Development 129(14):3513-22. [PubMed: 12091320] [MGI Ref ID J:77123]
Nayeem N; Kerr F; Naumann H; Linehan J; Lovestone S; Brandner S. 2007. Hyperphosphorylation of tau and neurofilaments and activation of CDK5 and ERK1/2 in PTEN-deficient cerebella. Mol Cell Neurosci 34(3):400-8. [PubMed: 17208451] [MGI Ref ID J:123240]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to noncarrier (wildtype) siblings. The donating investigator has not determined the phenotype of homozygous mice to date (January 2012).
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
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General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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