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| These mice carry a mutant allele of Stat3 (signal transducer and activator of transcription 3) that has mutations in exon 23 which disrupt an internal splice acceptor thereby preventing production of the beta isoform. Homozygotes are more susceptible to bacterial endotoxicity. This mutant mouse strain may be useful in studies of inflammatory response and septic (endotoxin) shock. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N11F7pN1
Generation DefinitionsDonating Investigator Stephen V. Desiderio, Johns Hopkins Medical Institute Description
These mice carry a mutant allele that has mutations in exon 23 (YIIITG) which disrupt an internal splice acceptor thereby preventing production of the beta isoform. These mice express only the alpha isoform. No beta isoform protein is detected in hepatic tissue or MEFs from homozygotes by RT-PCR. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are more susceptible to bacterial endotoxicity. Mutant mice are less likely to recover from LPS challenge than wildtype mice. This mutant mouse strain may be useful in studies of inflammatory response and septic (endotoxin) shock.Development
A targeting vector containing a loxP site flanked PGK-neo cassette was used to insert nucleotide substitutions in the polypyrimidine tract and AG dinucleotide of exon 23 (YIIITG), disrupting an internal splice acceptor and preventing production of the Stat3-beta isoform. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. ES cells that had successfully undergone Cre-mediated recombination and no longer retained the cassette were injected into C57BL/6 blastocysts. The resulting animals were backcrossed to 129X1/SvJ for 10 generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000691 129X1/SvJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Stat3
016923 B6.129S1-Stat3tm1Xyfu/J View Strains carrying other alleles of Stat3 (1 strain)
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Hyper-Ige Recurrent Infection Syndrome, Autosomal Dominant (STAT3)
assigned by genotype
Stat3tm1Desi/Stat3tm1Desi
involves: 129X1/SvJ * C57BL/6
- immune system phenotype
- increased susceptibility to endotoxin shock
- mutants challenged with bacterial lipopolysaccharide exhibit diminished recovery from endotoxic shock and exhibit severe acute tubular necrosis (MGI Ref ID J:77307)
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
Inflammation
Research Tools
Immunology and Inflammation Research
genes regulating susceptibility to infectious disease and endotoxin
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Stat3tm1Desi | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Stephen Desiderio | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Stat3Beta-; | ||
| Mutation Made By | Stephen Desiderio, Johns Hopkins Medical Institute | ||
| Strain of Origin | 129X1/SvJ | ||
| Gene Symbol and Name | Stat3, signal transducer and activator of transcription 3 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | 1110034C02Rik; APRF; AW109958; HIES; RIKEN cDNA 1110034C02 gene; expressed sequence AW109958; | ||
| Molecular Note | Exon 23 was replaced with modified sequence in which nucleotide susbtitutions within 3 polypyrimidine tracts and an AG dinucleotide were made in order to disrupt an internal splice acceptor site specific to the beta isoform. Transcript encoding the beta isoform was undetected by RT-PCR analysis of homozygous mutant hepatic mRNA, while wild-type levels of transcript encoding the alpha isoform were observed. Analysis of MEFs reiterated these findings at the protein level. [MGI Ref ID J:77307] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Stat3tm1Desi, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Yoo JY; Huso DL; Nathans D; Desiderio S. 2002. Specific ablation of Stat3beta distorts the pattern of Stat3-responsive gene expression and impairs recovery from endotoxic shock. Cell 108(3):331-44. [PubMed: 11853668] [MGI Ref ID J:77307]
Additional References
Stat3tm1Desi relatedLi H; Nourbakhsh B; Cullimore M; Zhang GX; Rostami A. 2011. IL-9 is important for T-cell activation and differentiation in autoimmune inflammation of the central nervous system. Eur J Immunol 41(8):2197-206. [PubMed: 21674475] [MGI Ref ID J:176816]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000691 129X1/SvJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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