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| These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek (Tie2) promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 16-JUL-09 Species laboratory mouse Generation N8+ (04-JUN-09) Donating Investigator Masashi Yanagisawa, Southwestern Medical School Description
These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek (Tie2) promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in vascular endothelial cells.Development
Cre cDNA and metallothionein-1 (MT-1) polyA signal sequence was inserted between the mouse Tek (Tie2) promoter and enhancer. The transgenic cassette was injected into B6SJLF1 fertilized oocytes and backcrossed to a C57BL/6 genetic background for at least eight generations by the donating investigator.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tek
004128 B6.Cg-Tg(Tek-cre)12Flv/J 008537 FVB-Tg(Tek-cre)2352Rwng/J 002856 FVB/N-Tg(TIE2-lacZ)182Sato/J 005707 STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J 006770 STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J 003658 STOCK Tg(TIE2GFP)287Sato/J View Strains carrying other alleles of Tek (6 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Tek-cre)1Ywa | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Masashi Yanagisawa | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | T2Cre; TekCre; Tie-2 Cre; Tie2-Cre; Tie2Cre; Tie2Cre+; | ||
| Mutation Made By | Masashi Yanagisawa, Southwestern Medical School | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Tek-cre)1Ywa, transgene insertion 1, Masashi Yanagisawa | ||
| Chromosome | UN | ||
| Gene Common Name(s) | T2Cre; TekCre; Tie-2 Cre; Tie2-Cre; Tie2Cre; Tie2Cre+; | ||
| Driver Note | Tek | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a mouse Tek promoter and enhancer. This promoter is active in endothelial cells. [MGI Ref ID J:67365] [MGI Ref ID J:71269] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Kisanuki YY; Hammer RE; Miyazaki J; Williams SC; Richardson JA; Yanagisawa M. 2001. Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo. Dev Biol 230(2):230-42. [PubMed: 11161575] [MGI Ref ID J:67365]
Additional References
Tg(Tek-cre)1Ywa relatedAki D; Minoda Y; Yoshida H; Watanabe S; Yoshida R; Takaesu G; Chinen T; Inaba T; Hikida M; Kurosaki T; Saeki K; Yoshimura A. 2008. Peptidoglycan and lipopolysaccharide activate PLCgamma2, leading to enhanced cytokine production in macrophages and dendritic cells. Genes Cells 13(2):199-208. [PubMed: 18233961] [MGI Ref ID J:138256]
Allende ML; Yamashita T; Proia RL. 2003. G-protein-coupled receptor S1P1 acts within endothelial cells to regulate vascular maturation. Blood 102(10):3665-7. [PubMed: 12869509] [MGI Ref ID J:86450]
Bagnall AJ; Kelland NF; Gulliver-Sloan F; Davenport AP; Gray GA; Yanagisawa M; Webb DJ; Kotelevtsev YV. 2006. Deletion of endothelial cell endothelin B receptors does not affect blood pressure or sensitivity to salt. Hypertension 48(2):286-93. [PubMed: 16801484] [MGI Ref ID J:135711]
Boulday G; Blecon A; Petit N; Chareyre F; Garcia LA; Niwa-Kawakita M; Giovannini M; Tournier-Lasserve E. 2009. Tissue-specific conditional CCM2 knockout mice establish the essential role of endothelial CCM2 in angiogenesis: implications for human cerebral cavernous malformations. Dis Model Mech 2(3-4):168-77. [PubMed: 19259391] [MGI Ref ID J:146210]
Camerer E; Regard JB; Cornelissen I; Srinivasan Y; Duong DN; Palmer D; Pham TH; Wong JS; Pappu R; Coughlin SR. 2009. Sphingosine-1-phosphate in the plasma compartment regulates basal and inflammation-induced vascular leak in mice. J Clin Invest 119(7):1871-9. [PubMed: 19603543] [MGI Ref ID J:152547]
Cattelino A; Liebner S; Gallini R; Zanetti A; Balconi G; Corsi A; Bianco P; Wolburg H; Moore R; Oreda B; Kemler R; Dejana E. 2003. The conditional inactivation of the beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility. J Cell Biol 162(6):1111-22. [PubMed: 12975353] [MGI Ref ID J:85505]
Cera MR; Del Prete A; Vecchi A; Corada M; Martin-Padura I; Motoike T; Tonetti P; Bazzoni G; Vermi W; Gentili F; Bernasconi S; Sato TN; Mantovani A; Dejana E. 2004. Increased DC trafficking to lymph nodes and contact hypersensitivity in junctional adhesion molecule-A-deficient mice. J Clin Invest 114(5):729-38. [PubMed: 15343392] [MGI Ref ID J:92592]
Chae SS; Paik JH; Allende ML; Proia RL; Hla T. 2004. Regulation of limb development by the sphingosine 1-phosphate receptor S1p1/EDG-1 occurs via the hypoxia/VEGF axis. Dev Biol 268(2):441-7. [PubMed: 15063179] [MGI Ref ID J:92203]
Chang S; Young BD; Li S; Qi X; Richardson JA; Olson EN. 2006. Histone deacetylase 7 maintains vascular integrity by repressing matrix metalloproteinase 10. Cell 126(2):321-34. [PubMed: 16873063] [MGI Ref ID J:116008]
Choudhary B; Zhou J; Li P; Thomas S; Kaartinen V; Sucov HM. 2009. Absence of TGFbeta signaling in embryonic vascular smooth muscle leads to reduced lysyl oxidase expression, impaired elastogenesis, and aneurysm. Genesis 47(2):115-21. [PubMed: 19165826] [MGI Ref ID J:147389]
Davies BS; Waki H; Beigneux AP; Farber E; Weinstein MM; Wilpitz DC; Tai LJ; Evans RM; Fong LG; Tontonoz P; Young SG. 2008. The expression of GHIBP1, an endothelial cell binding site for lipoprotein lipase and chylomicrons, is induced by peroxisome proliferator-activated receptor-gamma. Mol Endocrinol 22(11):2496-504. [PubMed: 18787041] [MGI Ref ID J:141250]
El Kasmi KC; Qualls JE; Pesce JT; Smith AM; Thompson RW; Henao-Tamayo M; Basaraba RJ; Konig T; Schleicher U; Koo MS; Kaplan G; Fitzgerald KA; Tuomanen EI; Orme IM; Kanneganti TD; Bogdan C; Wynn TA; Murray PJ. 2008. Toll-like receptor-induced arginase 1 in macrophages thwarts effective immunity against intracellular pathogens. Nat Immunol 9(12):1399-406. [PubMed: 18978793] [MGI Ref ID J:143229]
Ficara F; Murphy MJ; Lin M; Cleary ML. 2008. Pbx1 regulates self-renewal of long-term hematopoietic stem cells by maintaining their quiescence. Cell Stem Cell 2(5):484-96. [PubMed: 18462698] [MGI Ref ID J:149799]
French WJ; Creemers EE; Tallquist MD. 2008. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol 28(18):5646-57. [PubMed: 18606782] [MGI Ref ID J:140245]
Fu J; Gerhardt H; McDaniel JM; Xia B; Liu X; Ivanciu L; Ny A; Hermans K; Silasi-Mansat R; McGee S; Nye E; Ju T; Ramirez MI; Carmeliet P; Cummings RD; Lupu F; Xia L. 2008. Endothelial cell O-glycan deficiency causes blood/lymphatic misconnections and consequent fatty liver disease in mice. J Clin Invest 118(11):3725-37. [PubMed: 18924607] [MGI Ref ID J:144581]
Fuster MM; Wang L; Castagnola J; Sikora L; Reddi K; Lee PH; Radek KA; Schuksz M; Bishop JR; Gallo RL; Sriramarao P; Esko JD. 2007. Genetic alteration of endothelial heparan sulfate selectively inhibits tumor angiogenesis. J Cell Biol 177(3):539-49. [PubMed: 17470635] [MGI Ref ID J:134728]
Gitler AD; Kong Y; Choi JK; Zhu Y; Pear WS; Epstein JA. 2004. Tie2-Cre-induced inactivation of a conditional mutant Nf1 allele in mouse results in a myeloproliferative disorder that models juvenile myelomonocytic leukemia. Pediatr Res 55(4):581-4. [PubMed: 14739366] [MGI Ref ID J:88133]
Gitler AD; Zhu Y; Ismat FA; Lu MM; Yamauchi Y; Parada LF; Epstein JA. 2003. Nf1 has an essential role in endothelial cells. Nat Genet 33(1):75-9. [PubMed: 12469121] [MGI Ref ID J:80323]
Graupera M; Guillermet-Guibert J; Foukas LC; Phng LK; Cain RJ; Salpekar A; Pearce W; Meek S; Millan J; Cutillas PR; Smith AJ; Ridley AJ; Ruhrberg C; Gerhardt H; Vanhaesebroeck B. 2008. Angiogenesis selectively requires the p110alpha isoform of PI3K to control endothelial cell migration. Nature 453(7195):662-6. [PubMed: 18449193] [MGI Ref ID J:136236]
Grego-Bessa J; Luna-Zurita L; del Monte G; Bolos V; Melgar P; Arandilla A; Garratt AN; Zang H; Mukouyama YS; Chen H; Shou W; Ballestar E; Esteller M; Rojas A; Perez-Pomares JM; de la Pompa JL. 2007. Notch signaling is essential for ventricular chamber development. Dev Cell 12(3):415-29. [PubMed: 17336907] [MGI Ref ID J:119151]
Gu C; Rodriguez ER; Reimert DV; Shu T; Fritzsch B; Richards LJ; Kolodkin AL; Ginty DD. 2003. Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev Cell 5(1):45-57. [PubMed: 12852851] [MGI Ref ID J:93799]
Haigh JJ; Morelli PI; Gerhardt H; Haigh K; Tsien J; Damert A; Miquerol L; Muhlner U; Klein R; Ferrara N; Wagner EF; Betsholtz C; Nagy A. 2003. Cortical and retinal defects caused by dosage-dependent reductions in VEGF-A paracrine signaling. Dev Biol 262(2):225-41. [PubMed: 14550787] [MGI Ref ID J:86207]
Harvey NL; Srinivasan RS; Dillard ME; Johnson NC; Witte MH; Boyd K; Sleeman MW; Oliver G. 2005. Lymphatic vascular defects promoted by Prox1 haploinsufficiency cause adult-onset obesity. Nat Genet 37(10):1072-81. [PubMed: 16170315] [MGI Ref ID J:102652]
High FA; Lu MM; Pear WS; Loomes KM; Kaestner KH; Epstein JA. 2008. Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development. Proc Natl Acad Sci U S A 105(6):1955-9. [PubMed: 18245384] [MGI Ref ID J:143962]
Holtz ML; Misra RP. 2008. Endothelial-specific ablation of serum response factor causes hemorrhaging, yolk sac vascular failure, and embryonic lethality. BMC Dev Biol 8:65. [PubMed: 18570667] [MGI Ref ID J:139204]
Hou Y; Li F; Karin M; Ostrowski MC. 2008. Analysis of the IKKbeta/NF-kappaB signaling pathway during embryonic angiogenesis. Dev Dyn 237(10):2926-2935. [PubMed: 18816823] [MGI Ref ID J:139642]
Isermann B; Hendrickson SB; Zogg M; Wing M; Cummiskey M; Kisanuki YY; Yanagisawa M; Weiler H. 2001. Endothelium-specific loss of murine thrombomodulin disrupts the protein C anticoagulant pathway and causes juvenile-onset thrombosis. J Clin Invest 108(4):537-46. [PubMed: 11518727] [MGI Ref ID J:71269]
Ismat FA; Xu J; Lu MM; Epstein JA. 2006. The neurofibromin GAP-related domain rescues endothelial but not neural crest development in Nf1 mice. J Clin Invest 116(9):2378-84. [PubMed: 16906226] [MGI Ref ID J:114455]
Kaneko K; Li X; Zhang X; Lamberti JJ; Jamieson SW; Thistlethwaite PA. 2008. Endothelial expression of bone morphogenetic protein receptor type 1a is required for atrioventricular valve formation. Ann Thorac Surg 85(6):2090-8. [PubMed: 18498827] [MGI Ref ID J:137995]
Katz SG; Williams A; Yang J; Fujiwara Y; Tsang AP; Epstein JA; Orkin SH. 2003. Endothelial lineage-mediated loss of the GATA cofactor Friend of GATA 1 impairs cardiac development. Proc Natl Acad Sci U S A 100(24):14030-5. [PubMed: 14614148] [MGI Ref ID J:99745]
Khandoga A; Kessler JS; Meissner H; Hanschen M; Corada M; Motoike T; Enders G; Dejana E; Krombach F. 2005. Junctional adhesion molecule-A deficiency increases hepatic ischemia-reperfusion injury despite reduction of neutrophil transendothelial migration. Blood 106(2):725-33. [PubMed: 15827135] [MGI Ref ID J:107442]
Kimura A; Kinjyo I; Matsumura Y; Mori H; Mashima R; Harada M; Chien KR; Yasukawa H; Yoshimura A. 2004. SOCS3 is a physiological negative regulator for granulopoiesis and granulocyte colony-stimulating factor receptor signaling. J Biol Chem 279(8):6905-10. [PubMed: 14699146] [MGI Ref ID J:88961]
Kogata N; Arai Y; Pearson JT; Hashimoto K; Hidaka K; Koyama T; Somekawa S; Nakaoka Y; Ogawa M; Adams RH; Okada M; Mochizuki N. 2006. Cardiac ischemia activates vascular endothelial cadherin promoter in both preexisting vascular cells and bone marrow cells involved in neovascularization. Circ Res 98(7):897-904. [PubMed: 16543497] [MGI Ref ID J:121366]
Kondo T; Hafezi-Moghadam A; Thomas K; Wagner DD; Kahn CR. 2004. Mice lacking insulin or insulin-like growth factor 1 receptors in vascular endothelial cells maintain normal blood-brain barrier. Biochem Biophys Res Commun 317(2):315-20. [PubMed: 15063759] [MGI Ref ID J:89174]
Kondo T; Vicent D; Suzuma K; Yanagisawa M; King GL; Holzenberger M; Kahn CR. 2003. Knockout of insulin and IGF-1 receptors on vascular endothelial cells protects against retinal neovascularization. J Clin Invest 111(12):1835-42. [PubMed: 12813019] [MGI Ref ID J:84045]
Kono M; Mi Y; Liu Y; Sasaki T; Allende ML; Wu YP; Yamashita T; Proia RL. 2004. The sphingosine-1-phosphate receptors S1P1, S1P2, and S1P3 function coordinately during embryonic angiogenesis. J Biol Chem 279(28):29367-73. [PubMed: 15138255] [MGI Ref ID J:106055]
Kuhn M; Volker K; Schwarz K; Carbajo-Lozoya J; Flogel U; Jacoby C; Stypmann J; van Eickels M; Gambaryan S; Hartmann M; Werner M; Wieland T; Schrader J; Baba HA. 2009. The natriuretic peptide/guanylyl cyclase--a system functions as a stress-responsive regulator of angiogenesis in mice. J Clin Invest 119(7):2019-30. [PubMed: 19487812] [MGI Ref ID J:152580]
Lacy-Hulbert A; Smith AM; Tissire H; Barry M; Crowley D; Bronson RT; Roes JT; Savill JS; Hynes RO. 2007. Ulcerative colitis and autoimmunity induced by loss of myeloid alphav integrins. Proc Natl Acad Sci U S A 104(40):15823-8. [PubMed: 17895374] [MGI Ref ID J:125508]
Lei L; Liu D; Huang Y; Jovin I; Shai SY; Kyriakides T; Ross RS; Giordano FJ. 2008. Endothelial expression of beta1 integrin is required for embryonic vascular patterning and postnatal vascular remodeling. Mol Cell Biol 28(2):794-802. [PubMed: 17984225] [MGI Ref ID J:130230]
Limbourg FP; Takeshita K; Radtke F; Bronson RT; Chin MT; Liao JK. 2005. Essential role of endothelial Notch1 in angiogenesis. Circulation 111(14):1826-32. [PubMed: 15809373] [MGI Ref ID J:100449]
Lincoln J; Kist R; Scherer G; Yutzey KE. 2007. Sox9 is required for precursor cell expansion and extracellular matrix organization during mouse heart valve development. Dev Biol 305(1):120-32. [PubMed: 17350610] [MGI Ref ID J:121352]
Luo Y; Radice GL. 2005. N-cadherin acts upstream of VE-cadherin in controlling vascular morphogenesis. J Cell Biol 169(1):29-34. [PubMed: 15809310] [MGI Ref ID J:98422]
Ma Q; Zhou B; Pu WT. 2008. Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity. Dev Biol 323(1):98-104. [PubMed: 18775691] [MGI Ref ID J:141152]
Maddaluno L; Verbrugge SE; Martinoli C; Matteoli G; Chiavelli A; Zeng Y; Williams ED; Rescigno M; Cavallaro U. 2009. The adhesion molecule L1 regulates transendothelial migration and trafficking of dendritic cells. J Exp Med 206(3):623-35. [PubMed: 19273627] [MGI Ref ID J:146748]
Matsumura Y; Kobayashi T; Ichiyama K; Yoshida R; Hashimoto M; Takimoto T; Tanaka K; Chinen T; Shichita T; Wyss-Coray T; Sato K; Yoshimura A. 2007. Selective expansion of foxp3-positive regulatory T cells and immunosuppression by suppressors of cytokine signaling 3-deficient dendritic cells. J Immunol 179(4):2170-9. [PubMed: 17675476] [MGI Ref ID J:151229]
McCarty JH; Lacy-Hulbert A; Charest A; Bronson RT; Crowley D; Housman D; Savill J; Roes J; Hynes RO. 2005. Selective ablation of {alpha}v integrins in the central nervous system leads to cerebral hemorrhage, seizures, axonal degeneration and premature death. Development 132(1):165-176. [PubMed: 15576410] [MGI Ref ID J:94376]
Merki E; Zamora M; Raya A; Kawakami Y; Wang J; Zhang X; Burch J; Kubalak SW; Kaliman P; Belmonte JC; Chien KR; Ruiz-Lozano P. 2005. Epicardial retinoid X receptor alpha is required for myocardial growth and coronary artery formation. Proc Natl Acad Sci U S A 102(51):18455-60. [PubMed: 16352730] [MGI Ref ID J:104700]
Montoyo HP ; Vaccaro C ; Hafner M ; Ober RJ ; Mueller W ; Ward ES. 2009. Conditional deletion of the MHC class I-related receptor FcRn reveals the sites of IgG homeostasis in mice. Proc Natl Acad Sci U S A 106(8):2788-93. [PubMed: 19188594] [MGI Ref ID J:146640]
Mukouyama YS; Gerber HP; Ferrara N; Gu C; Anderson DJ. 2005. Peripheral nerve-derived VEGF promotes arterial differentiation via neuropilin 1-mediated positive feedback. Development 132(5):941-952. [PubMed: 15673567] [MGI Ref ID J:96924]
Navankasattusas S; Whitehead KJ; Suli A; Sorensen LK; Lim AH; Zhao J; Park KW; Wythe JD; Thomas KR; Chien CB; Li DY. 2008. The netrin receptor UNC5B promotes angiogenesis in specific vascular beds. Development 135(4):659-67. [PubMed: 18223200] [MGI Ref ID J:131817]
Park EJ; Watanabe Y; Smyth G; Miyagawa-Tomita S; Meyers E; Klingensmith J; Camenisch T; Buckingham M; Moon AM. 2008. An FGF autocrine loop initiated in second heart field mesoderm regulates morphogenesis at the arterial pole of the heart. Development 135(21):3599-610. [PubMed: 18832392] [MGI Ref ID J:143444]
Ramakrishna S; Kim IM; Petrovic V; Malin D; Wang IC; Kalin TV; Meliton L; Zhao YY; Ackerson T; Qin Y; Malik AB; Costa RH; Kalinichenko VV. 2007. Myocardium defects and ventricular hypoplasia in mice homozygous null for the Forkhead Box m1 transcription factor. Dev Dyn 236(4):1000-1013. [PubMed: 17366632] [MGI Ref ID J:119481]
Rivera-Feliciano J; Lee KH; Kong SW; Rajagopal S; Ma Q; Springer Z; Izumo S; Tabin CJ; Pu WT. 2006. Development of heart valves requires Gata4 expression in endothelial-derived cells. Development 133(18):3607-18. [PubMed: 16914500] [MGI Ref ID J:112458]
Robertson EJ; Charatsi I; Joyner CJ; Koonce CH; Morgan M; Islam A; Paterson C; Lejsek E; Arnold SJ; Kallies A; Nutt SL; Bikoff EK. 2007. Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice. Development 134(24):4335-45. [PubMed: 18039967] [MGI Ref ID J:129204]
Ruppel KM; Willison D; Kataoka H; Wang A; Zheng YW; Cornelissen I; Yin L; Xu SM; Coughlin SR. 2005. Essential role for Galpha13 in endothelial cells during embryonic development. Proc Natl Acad Sci U S A 102(23):8281-6. [PubMed: 15919816] [MGI Ref ID J:99731]
Sabrane K; Kruse MN; Fabritz L; Zetsche B; Mitko D; Skryabin BV; Zwiener M; Baba HA; Yanagisawa M; Kuhn M. 2005. Vascular endothelium is critically involved in the hypotensive and hypovolemic actions of atrial natriuretic peptide. J Clin Invest 115(6):1666-1674. [PubMed: 15931395] [MGI Ref ID J:99197]
Schlaeger TM; Mikkola HK; Gekas C; Helgadottir HB; Orkin SH. 2005. Tie2Cre-mediated gene ablation defines the stem-cell leukemia gene (SCL/tal1)-dependent window during hematopoietic stem-cell development. Blood 105(10):3871-4. [PubMed: 15677556] [MGI Ref ID J:98971]
Schreiber T; Ehlers S; Heitmann L; Rausch A; Mages J; Murray PJ; Lang R; Holscher C. 2009. Autocrine IL-10 induces hallmarks of alternative activation in macrophages and suppresses antituberculosis effector mechanisms without compromising T cell immunity. J Immunol 183(2):1301-12. [PubMed: 19561100] [MGI Ref ID J:151659]
Schreier B; Borner S; Volker K; Gambaryan S; Schafer SC; Kuhlencordt P; Gassner B; Kuhn M. 2008. The heart communicates with the endothelium through the guanylyl cyclase-A receptor: acute handling of intravascular volume in response to volume expansion. Endocrinology 149(8):4193-9. [PubMed: 18450968] [MGI Ref ID J:145509]
Schwarz Q; Vieira JM; Howard B; Eickholt BJ; Ruhrberg C. 2008. Neuropilin 1 and 2 control cranial gangliogenesis and axon guidance through neural crest cells. Development 135(9):1605-13. [PubMed: 18356247] [MGI Ref ID J:134486]
Shiraishi H; Yoshida H; Saeki K; Miura Y; Watanabe S; Ishizaki T; Hashimoto M; Takaesu G; Kobayashi T; Yoshimura A. 2008. Prostaglandin E2 is a major soluble factor produced by stromal cells for preventing inflammatory cytokine production from dendritic cells. Int Immunol 20(9):1219-29. [PubMed: 18640970] [MGI Ref ID J:139035]
Shrimali RK; Weaver JA; Miller GF; Starost MF; Carlson BA; Novoselov SV; Kumaraswamy E; Gladyshev VN; Hatfield DL. 2007. Selenoprotein expression is essential in endothelial cell development and cardiac muscle function. Neuromuscul Disord 17(2):135-42. [PubMed: 17142041] [MGI Ref ID J:129594]
Snarr BS; O'Neal JL; Chintalapudi MR; Wirrig EE; Phelps AL; Kubalak SW; Wessels A. 2007. Isl1 expression at the venous pole identifies a novel role for the second heart field in cardiac development. Circ Res 101(10):971-4. [PubMed: 17947796] [MGI Ref ID J:141329]
Sotiropoulos KB; Clermont A; Yasuda Y; Rask-Madsen C; Mastumoto M; Takahashi J; Della Vecchia K; Kondo T; Aiello LP; King GL. 2006. Adipose-specific effect of rosiglitazone on vascular permeability and protein kinase C activation: novel mechanism for PPARgamma agonist's effects on edema and weight gain. FASEB J 20(8):1203-5. [PubMed: 16672634] [MGI Ref ID J:111347]
Srinivasan RS; Dillard ME; Lagutin OV; Lin FJ; Tsai S; Tsai MJ; Samokhvalov IM; Oliver G. 2007. Lineage tracing demonstrates the venous origin of the mammalian lymphatic vasculature. Genes Dev 21(19):2422-2432. [PubMed: 17908929] [MGI Ref ID J:125507]
Stankunas K; Hang CT; Tsun ZY; Chen H; Lee NV; Wu JI; Shang C; Bayle JH; Shou W; Iruela-Arispe ML; Chang CP. 2008. Endocardial Brg1 represses ADAMTS1 to maintain the microenvironment for myocardial morphogenesis. Dev Cell 14(2):298-311. [PubMed: 18267097] [MGI Ref ID J:132583]
Suarez Y; Fernandez-Hernando C; Yu J; Gerber SA; Harrison KD; Pober JS; Iruela-Arispe ML; Merkenschlager M; Sessa WC. 2008. Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis. Proc Natl Acad Sci U S A 105(37):14082-7. [PubMed: 18779589] [MGI Ref ID J:143941]
Tanaka K; Ichiyama K; Hashimoto M; Yoshida H; Takimoto T; Takaesu G; Torisu T; Hanada T; Yasukawa H; Fukuyama S; Inoue H; Nakanishi Y; Kobayashi T; Yoshimura A. 2008. Loss of Suppressor of Cytokine Signaling 1 in Helper T Cells Leads to Defective Th17 Differentiation by Enhancing Antagonistic Effects of IFN-{gamma} on STAT3 and Smads. J Immunol 180(6):3746-56. [PubMed: 18322180] [MGI Ref ID J:132905]
Tang N; Mack F; Haase VH; Simon MC; Johnson RS. 2006. pVHL function is essential for endothelial extracellular matrix deposition. Mol Cell Biol 26(7):2519-30. [PubMed: 16537898] [MGI Ref ID J:106926]
Tang N; Wang L; Esko J; Giordano FJ; Huang Y; Gerber HP; Ferrara N; Johnson RS. 2004. Loss of HIF-1alpha in endothelial cells disrupts a hypoxia-driven VEGF autocrine loop necessary for tumorigenesis. Cancer Cell 6(5):485-95. [PubMed: 15542432] [MGI Ref ID J:94773]
Tanjore H; Zeisberg EM; Gerami-Naini B; Kalluri R. 2008. Beta1 integrin expression on endothelial cells is required for angiogenesis but not for vasculogenesis. Dev Dyn 237(1):75-82. [PubMed: 18058911] [MGI Ref ID J:130422]
Tanowitz HB; Huang H; Jelicks LA; Chandra M; Loredo ML; Weiss LM; Factor SM; Shtutin V; Mukherjee S; Kitsis RN; Christ GJ; Wittner M; Shirani J; Kisanuki YY; Yanagisawa M. 2005. Role of endothelin 1 in the pathogenesis of chronic chagasic heart disease. Infect Immun 73(4):2496-503. [PubMed: 15784596] [MGI Ref ID J:97201]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred. Mating System Noncarrier x Hemizygote (Female x Male) 16-JUL-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Tek-cre)1Ywa
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Tek-cre)1Ywa x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Tek-cre)1Ywa
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Tek-cre)1Ywa
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Tek-cre)1Ywa x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Tek-cre)1Ywa
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.