Strain Name:

STOCK En2tm6Alj/J

Stock Number:

008872

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These En2lox mice have loxP sites flanking the engrailed homeodomain portion in exon 2 of the engrailed 2 gene. These mice may be used to generate conditional mutations for studying engrailed protein function in the developing mesencephalon, rhombomere 1, and jaw muscles, as well as the embryonic and adult cerebellum.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation?pN1
Generation Definitions
 
Donating InvestigatorDr. Alexandra L Joyner,   Memorial Sloan-Kettering Cancer Center

Description
Mice homozygous for the En2lox conditional allele are viable and fertile, with loxP sites flanking the coding region of exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region (encoding the engrailed homeodomain) deleted in the cre-expressing tissues. These En2lox mice may be useful in generating conditional mutations for studying engrailed protein function in the developing mesencephalon, rhombomere 1, and jaw muscles, as well as the embryonic and adult cerebellum.

Development
A targeting construct was designed to insert an frt-flanked neomycin cassette and loxP site within intron 1, and a loxP site downstream of the coding region in the second exon of the targeted gene. This construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss outbred mice. The resulting En2neo-lox mice were then bred to C57BL/6-congenic mice constitutively expressing Flp recombinase (hACTB-Flpe deleter mice; see Stock No. 005703) to remove the frt-flanked neo cassette; thus generating En2lox offspring. En2lox mutant mice were bred together or to Swiss Webster mice for many generations (and the Flp transgene was removed) prior to sending to The Jackson Laboratory Repository in 2013. In addition, the donating investigator reports that although these mice harbor no additional mutant loci, they may contain incidental genetic background contributions as a result of past matings with engrailed-1 mutant lines and/or Cre recombinase-expressing lines. Upon arrival, sperm was cryopreserved. To generate the living En2lox colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J female mice (Stock No. 000664). The En2lox colony was then maintained by breeding heterozygous or homozygous mice together, to wildtype mice from the colony or to C57BL/6J inbred mice.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of En2
002657   B6;129S2-En2tm1Alj/J
007924   STOCK En2tm4(cre/ERT2)Alj/J
007925   STOCK En2tm5.1Alj/J
008852   STOCK Tg(En2-cre)22Alj/J
View Strains carrying other alleles of En2     (4 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol En2tm6Alj
Allele Name targeted mutation 6, Alexandra L Joyner
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) En2flox; En2lox;
Mutation Made ByDr. Alexandra Joyner,   Memorial Sloan-Kettering Cancer Center
Strain of Origin129S6/SvEvTac
Gene Symbol and Name En2, engrailed 2
Chromosome 5
Gene Common Name(s) BB131122; En-2; RGD1561842; expressed sequence BB131122;
Molecular Note The targeting construct contains a 3.1 kb En2 5' fragment, an Frt-flanked PGK-neo cassette followed by a LoxP site in the En2 intron and a 4.8 kb En2 genomic fragment containing a LoxP site in the XbaI site of the 3' UTR. Flpe-mediated recombination deleted the PGK-neo, producing an En2 allele with a floxed region. [MGI Ref ID J:156169]

Genotyping

Genotyping Information

Genotyping Protocols

En2tm6Alj, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cheng Y; Sudarov A; Szulc KU; Sgaier SK; Stephen D; Turnbull DH; Joyner AL. 2010. The Engrailed homeobox genes determine the different foliation patterns in the vermis and hemispheres of the mammalian cerebellum. Development 137(3):519-29. [PubMed: 20081196]  [MGI Ref ID J:156169]

Additional References

En2tm6Alj related

Fox SR; Deneris ES. 2012. Engrailed is required in maturing serotonin neurons to regulate the cytoarchitecture and survival of the dorsal raphe nucleus. J Neurosci 32(23):7832-42. [PubMed: 22674259]  [MGI Ref ID J:185187]

Orvis GD; Hartzell AL; Smith JB; Barraza LH; Wilson SL; Szulc KU; Turnbull DH; Joyner AL. 2012. The engrailed homeobox genes are required in multiple cell lineages to coordinate sequential formation of fissures and growth of the cerebellum. Dev Biol 367(1):25-39. [PubMed: 22564796]  [MGI Ref ID J:185802]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). As the donating investigator reports that homozygous mice are viable and fertile, the colony may also be maintained by breeding homozygous mice together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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