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| These Ple176-EGFPCre;mEMS759 mice have the Ple176-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. As the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene direct expression of an EGFPCre fusion protein to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina, these Ple176-EGFPCre;mEMS759 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.129P2-Hprt1tm11(Ple176-EGFP/cre)Ems/J (Changed: 20-OCT-09 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System See Colony Maintenance Species laboratory mouse Generation N3+ (20-APR-09) Donating Investigator Elizabeth Simpson, University of British Columbia Description
These Ple176-EGFPCre;mEMS759 mice have the Ple176-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre). These Ple176-EGFPCre;mEMS759 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.For Ple176-EGFPCre;mEMS759 expression information, see The Pleiades Promoter Project website (Ple176 Promoter (pEMS1088)).
The donating investigator reports:
When these Ple176-EGFPCre;mEMS759 mice are bred to also harbor the Gt(ROSA)26Sortm1Sor mutant allele, strong Cre recombinase activity (βGal staining) is detected widely throughout the brain and eye, with notable expression in the hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum and retina. In each case, the staining appears mosaic, thus not all cells are βGal-positive, but a significant subpopulation of cells across many regions are labeled. This mosaicism is emphasized in the labeling of radial columns of cells in the cortex and the retina. A majority of the βGal-positive cells of the grey matter appear to be neuronal, as they co-label with the neuronal marker NeuN. However, it is apparent that the reporter is not restricted to neurons, but is also detected in cells within the white matter (notably in the corpus callosum, hippocampal fimbria, and the cerebellar white matter), suggestive of glial labeling as well. EGFP expression was not detected in any cells of the adult brain, indicating βGal expression is an historical marker of expression of the EGFP/cre reporter. The pattern of Cre recombinase activity (βGal expression) is very similar to that seen for the Ple177 (RGS16-C; Stock No. 008877) and the Ple178 (RGS16-D; Stock No. 008709) strains.These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia); their goal is to generate 160 fully characterized, human DNA promoters of less than 4 kb (MiniPromoters) to drive gene expression in defined brain regions of therapeutic interest for studying disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Amyotrophic Lateral Sclerosis (Lou Gehrig's disease), Multiple Sclerosis, Spinocerebellar Ataxia, Depression, Autism, and Cancer.
Development
The Ple176-EGFPCre transgene (pEMS1088) was designed with the 3231 bp Ple176 minipromoter (RGS16-B; derived from two upstream candidate regulatory regions and a subsection of the human regulator of G-protein signaling 16 (RGS16) promoter) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt1b-m3 mutant locus on the X chromosome via electroporation into mEMS21TG2A embryonic stem cells (derived from 129P2/OlaHsd-derived E14TG2a ES cells; a karyotypically male ES cell line harboring the Hprt1b-m3 mutation on the X chromosome). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric mice (founder line mEMS759) were bred to B6.129S4-Gt(ROSA)26Sortm1Sor/J mice (Stock No. 003474) to establish the mutant colony. These Ple176-EGFPCre;mEMS759 mutant mice were bred with B6.129S4-Gt(ROSA)26Sortm1Sor/J mice for multiple generations. After this, hemizygous sperm was sent to The Jackson Laboratory. Upon arrival, Ple176-EGFPCre;mEMS759 mutant mice were backcrossed to C57BL/6J inbred mice (selecting away from the Gt(ROSA)26Sortm1Sor mutation) to establish this congenic strain.A 27 SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed 6 of 27 markers that were not C57BL/6 allele-type. Therefore this strain is not congenic on a C57BL/6 genetic background.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
009114 B6.129P2(129S4)-Hprt1tm14(Ple103-EGFP/cre)Ems/J 008710 B6.129P2-Hprt1tm10(Ple162-EGFP/cre)Ems/J 008877 B6.129P2-Hprt1tm12(Ple177-EGFP/cre)Ems/J 008709 B6.129P2-Hprt1tm9(Ple178-EGFP/cre)Ems/J
004302 129S1-Hprt1tm1(cre)Mnn/J 002027 129S8/SvEv-Gpi1c Hprt1b-m2/J 002171 B6.129P2-Hprt1b-m3/J 008710 B6.129P2-Hprt1tm10(Ple162-EGFP/cre)Ems/J 008877 B6.129P2-Hprt1tm12(Ple177-EGFP/cre)Ems/J 008706 B6.129P2-Hprt1tm4(Ple88-EGFP)Ems/J 008707 B6.129P2-Hprt1tm7(Ple185-EGFP)Ems/J 008708 B6.129P2-Hprt1tm8(Ple151-EGFP)Ems/J 008709 B6.129P2-Hprt1tm9(Ple178-EGFP/cre)Ems/J 003138 B6;129-Hprt1tm1Detl/J 000807 RBJ/DnJ
008877 B6.129P2-Hprt1tm12(Ple177-EGFP/cre)Ems/J 008709 B6.129P2-Hprt1tm9(Ple178-EGFP/cre)Ems/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Hprt1tm11(Ple176-EGFP/cre)Ems | ||
|---|---|---|---|
| Allele Name | targeted mutation 11, Elizabeth M Simpson | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Hprt1tm11(Ple176-EGFPcre;mEMS759)Ems; Hprt1tm11(mEMS759)Ems; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | mEMS21TG2A | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | RGS16, regulator of G-protein signaling 16, human | ||
| Driver Note | Ple167 | ||
| Molecular Note | The Ple176-EGFPCre transgene (pEMS1088) was designed with the 3231 bp Ple176 minipromoter (RGS16-B; derived from two upstream candidate regulatory regions and a subsection of the human regulator of G-protein signaling 16 (/RGS16/) promoter) upstream of aminimal F5 mutant-/frt/ site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal /frt/ site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). | ||
| Gene Symbol and Name | Hprt1, hypoxanthine guanine phosphoribosyl transferase 1 | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; HPGRT; HPRT; Hgprtase; MGC112554; expressed sequence C81579; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Hprt1tm11(ple176-EGFP/cre), Standard PCR
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
The Pleiades Promoter Project, URL: http://pleiades.org/index.php. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data Submission :. [MGI Ref ID J:145689]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males (Stock No. 000664). Mating System See above Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 09-NOV-09
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Male Hemizygous for Hprt1tm11(Ple176-EGFP/cre)Ems $243.50 Female Heterozygous for Hprt1tm11(Ple176-EGFP/cre)Ems
Pairs /Price (US dollars $) Pair Genotype $487.00 Heterozygous for Hprt1tm11(Ple176-EGFP/cre)Ems x Hemizygous for Hprt1tm11(Ple176-EGFP/cre)Ems
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Male Hemizygous for Hprt1tm11(Ple176-EGFP/cre)Ems $316.60 Female Heterozygous for Hprt1tm11(Ple176-EGFP/cre)Ems
Pairs /Price (US dollars $) Pair Genotype $633.10 Heterozygous for Hprt1tm11(Ple176-EGFP/cre)Ems x Hemizygous for Hprt1tm11(Ple176-EGFP/cre)Ems
Additional Supply Details
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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