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| These ROSA26-Syn-E46K mice allow cre-inducible expression of the E46K missense mutant form of human alpha-synuclein (human E46K α-Syn or SYNE46K) associated with familial Parkinson's disease, dementia, and visual hallucinations, and may be useful for studying the progressive dopaminergic neurodegeneration of Parkinson's disease and other synucleinopathies, Lewy bodies, and synaptic plasticity. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 16-SEP-09 Species laboratory mouse Generation F?+3 (18-NOV-09) Donating Investigator Darren Moore, Ecole Polytechnique Federale de Lausanne Donating Investigator Ted Dawson, Johns Hopkins Univ School of Medicine Description
Homozygous ROSA26-Syn-E46K mice are viable and fertile, with the E46K missense mutant form of human alpha-synuclein (human E46K α-Syn or SYNE46K; associated with familial Parkinson's disease, dementia, and visual hallucinations) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of human E46K α-Syn is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no human E46K α-Syn protein is observed in brain regions). When bred to cre expressing mice, the STOP sequence is deleted in the tissues of offspring where Cre recombinase is present; resulting in human E46K α-Syn expression. In particular, Stock No. 008601 B6.Cg-Tg(Th-cre)1Tmd/J may be useful for this application. These ROSA26-Syn-E46K mice allow inducible expression of a human mutation associated with familial Parkinson's disease, dementia, and visual hallucinations and may be useful for studying the progressive dopaminergic neurodegeneration of Parkinson's disease and other synucleinopathies, Lewy bodies, and synaptic plasticity.Development
A targeting vector was designed with an SV40 splice acceptor, a loxP-flanked STOP cassette (PGK-neo and triple SV40 transcriptional termination sequence), a human alpha-synuclein cDNA bearing a familial Parkinson's disease-linked E46K missense mutation (SYNE46K), and a bovine polyadenylation signal. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation into into 129/SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6J mice to generate the mutant colony. ROSA26-Syn-E46K mice were bred to C57BL/6 for 2-3 generations and then bred together to make homozygous prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (64 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Gt(ROSA)26Sortm3(SNCA*E46K)Djmo/Gt(ROSA)26Sortm3(SNCA*E46K)Djmo
involves: 129 * C57BL/6J
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:147333)
- mice are viable and fertile
This mouse can be used to support research in many areas including:
Neurobiology Research
Ataxia (Movement) Defects
Cre-lox System
loxP-flanked Sequences
Neurodegeneration
Neurotransmitter Receptor and Synaptic Vesicle Defects
Parkinson's Disease
synuclein mutants
Research Tools
Cre-lox System
loxP-flanked Sequences
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Neurobiology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm3(SNCA*E46K)Djmo | ||
|---|---|---|---|
| Allele Name | Targeted mutation 3, Darren Moore | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | ROSA26-Syn-E46K; ROSA26-SynE46KTg; | ||
| Mutation Made By | Darren Moore, Ecole Polytechnique Federale de Lausanne | ||
| Strain of Origin | 129 | ||
| ES Cell Line Name | Other (see notes) | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| General Note | Authors state that 129/SvJ-derived embryonic stem cells were used. | ||
| Molecular Note | A targeting vector was designed with an SV40 splice acceptor, a loxP-flanked STOP cassette (PGK-neo and triple SV40 transcriptional termination sequence), a human alpha-synuclein cDNA bearing a familial Parkinson's disease-linked E46K missense mutation (SYNE46K), and a bovine polyadenylation signal. [MGI Ref ID J:147333] [MGI Ref ID J:150777] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Daher JP; Ying M; Banerjee R; McDonald RS; Dumas Hahn M; Yang L; Beal MF; Thomas B; Dawson VL; Dawson TM; Moore DJ. 2009. Conditional transgenic mice expressing C-terminally truncated human alpha-synuclein (alphaSyn119) exhibit reduced striatal dopamine without loss of nigrostriatal pathway dopaminergic neurons. Mol Neurodegener 4(1):34. [PubMed: 19630976] [MGI Ref ID J:150777]
Additional References
Gt(ROSA)26Sortm3(SNCA*E46K)Djmo relatedMoore D. 2009. Generation of a conditional knockin allele expressing the human alpha-synuclein sequence with the E46K mutation MGI Direct Data Submission :. [MGI Ref ID J:147333]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 16-SEP-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Gt(ROSA)26Sortm3(SNCA*E46K)Djmo
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Gt(ROSA)26Sortm3(SNCA*E46K)Djmo x Homozygous for Gt(ROSA)26Sortm3(SNCA*E46K)Djmo
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Gt(ROSA)26Sortm3(SNCA*E46K)Djmo
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Gt(ROSA)26Sortm3(SNCA*E46K)Djmo x Homozygous for Gt(ROSA)26Sortm3(SNCA*E46K)Djmo
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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