Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   17-NOV-09 Species laboratory mouse Generation N12pN1 Generation Definitions   Donating Investigator Xiao-Ming Yin,   Univ. of Pittsburgh School of Medicine

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by Western blot analysis of spleen and thymus tissue. Most homozygotes survive injection of anti-Fas antibodies while wildtype mice succumb to acute liver failure within hours. Mutant mice are resistant to hypoxia-ischemia (HI) injury, experimentally induced apoptosis, and oxygen toxicity under hyperoxic conditions. Homozygotes exhibit reduced lysosome permeability and diminished reactive oxygen species generation. Isolated neurons are resistant to oxygen/glucose deprivation induced cell death. This mutant mouse strain may be useful in studies of mitochondiral activation in apoptosis, and ischemic cell injury and death.

Development
A targeting vector containing a PGK-neo cassette was used to disrupt exons 1 and 2 and part of exon 3. The construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 12 generations before arriving at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Bid^tm1Sjk/Bid^tm1Sjk

        B6.129X1-Bid^tm1Sjk

• nervous system phenotype
• abnormal hippocampus morphology
  • 2 days after neonatal hypoxia-ischemia (HI) injury, ispsilateral hippocampal area loss is not decreased relative to that observed in wild-type neonates   (MGI Ref ID J:111236)
• gliosis
  • reactive gliosis is significantly decreased in hippocampi of mutants 2 days after neonatal HI compared to wild-type   (MGI Ref ID J:111236)
• respiratory system phenotype
• *normal* respiratory system phenotype
  • after bleomycin treatment, mutant lungs show similar inflammatory response over same time course as observed for wild-type lungs   (MGI Ref ID J:107667)
  • mouse alveolar epithelial cells show similar levels of cell death to controls after bleomycin treatment   (MGI Ref ID J:107667)
  • lung fibroblasts show proliferation rates similar to wild-type under low serum conditions   (MGI Ref ID J:107667)
• • abnormal pulmonary alveolus epithelial cell morphology
    • no cell death is observed when cells are treated with Tgfb1, whereas cell death is induced in wild-type cells   (MGI Ref ID J:107667)
• homeostasis/metabolism phenotype
• *normal* homeostasis/metabolism phenotype
  • after bleomycin treatment, Tgfb1 levels in brochoalveolar lavage fluid are not different from control values   (MGI Ref ID J:107667)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Bid^tm1Sjk/Bid^tm1Sjk

        involves: 129X1/SvJ

• mortality/aging
• decreased sensitivity to induced morbidity/mortality
  • unlike wild-type mice that die from hepatocellular apoptosis and hemorrhagic necrosis after injection of an anti-Fas antibody, nearly all mutants survive   (MGI Ref ID J:57073)
• homeostasis/metabolism phenotype
• decreased susceptibility to injury
  • animals survive with little or no liver damage after injection with anti-Fas antibodies   (MGI Ref ID J:57073)
• cellular phenotype
• abnormal mitochondrial physiology
  • the transmembrane potential of mitochondria is better maintained in mutant MEFs treated with TNF-alpha or thymocytes treated with anti-Fas antibody than wild-type cells   (MGI Ref ID J:57073)
• decreased apoptosis
  • mutants are resistant to Fas-induced hepatocellular apoptosis   (MGI Ref ID J:57073)

Bid^tm1Sjk/Bid^tm1Sjk

        involves: 129X1/Sv * C57BL/6

• mortality/aging
• decreased sensitivity to induced morbidity/mortality
  • when placed under hyperoxic conditions for more than 5 days, mice show increased survival (resistance to oxygen toxicity) compared to wild-type mice   (MGI Ref ID J:120650)
• respiratory system phenotype
• abnormal lung morphology
  • lung fibroblasts show increased resistance (less apoptosis) to hyperoxia than wild-type fibroblasts   (MGI Ref ID J:120650)
• endocrine/exocrine gland phenotype
• abnormal pancreatic beta cell morphology
  • treatment of islet cells with perforin/granzyme B does not result in cytochrome C release as seen in wild-type cells; release is blocked in Bid-null islets   (MGI Ref ID J:116520)
  • islet cells are resistant to granzyme B-mediated apoptosis, unlike wild-type cells   (MGI Ref ID J:116520)
• liver/biliary system phenotype
• *normal* liver/biliary system phenotype
  • hepatocytes show normal sensitivity to Tnf-mediated hepatocyte destruction   (MGI Ref ID J:132334)

Bid^tm1Sjk/Bid^tm1Sjk

        involves: 129X1/SvJ * C57BL/6

• immune system phenotype
• decreased susceptibility to endotoxin shock
  • LPS-GalN-induced liver injury, mice exhibit reduced liver injury and mortality during the early phase   (MGI Ref ID J:117693)
  • however, late phase response is normal and mice exhibit the same mortality as wild-type mice   (MGI Ref ID J:117693)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research

Research Tools
Apoptosis Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Bidtm1Sjk
Allele Name		targeted mutation 1, Stanley J Korsmeyer
Allele Type		Targeted (knock-out)
Common Name(s)		Bid-;
Mutation Made By		Jane Chen,   Univ. of Pittsburgh School of Medicine
Strain of Origin		129X1/SvJ
ES Cell Line Name		RW-4
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Bid, BH3 interacting domain death agonist
Chromosome		6
Gene Common Name(s)		2700049M22Rik; AI875481; AU022477; FP497; RIKEN cDNA 2700049M22 gene; expressed sequence AI875481; expressed sequence AU022477;
Molecular Note		A 4.2 kb genomic fragment containing exons 1, 2 and part of 3 was replaced with a neomycin selection cassette inserted in reverse transcriptional orientation to the gene. The deleted sequences encode the BH3 domain of the protein. Western blot analysison spleen and thymus samples derived from homozygous mice demonstrated that no detectable protein is produced from this allele. [MGI Ref ID J:57073]

Genotyping

Genotyping Information

Genotyping Protocols

Bid^tm1Sjk, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Yin XM; Wang K; Gross A; Zhao Y; Zinkel S; Klocke B; Roth KA; Korsmeyer SJ. 1999. Bid-deficient mice are resistant to Fas-induced hepatocellular apoptosis. Nature 400(6747):886-91. [PubMed: 10476969]  [MGI Ref ID J:57073]

Additional References

Bid^tm1Sjk related

Bai L; Ni HM; Chen X; Difrancesca D; Yin XM. 2005. Deletion of bid impedes cell proliferation and hepatic carcinogenesis. Am J Pathol 166(5):1523-32. [PubMed: 15855651]  [MGI Ref ID J:98464]

Bonzon C; Bouchier-Hayes L; Pagliari LJ; Green DR; Newmeyer DD. 2006. Caspase-2-induced apoptosis requires bid cleavage: a physiological role for bid in heat shock-induced death. Mol Biol Cell 17(5):2150-7. [PubMed: 16495337]  [MGI Ref ID J:112537]

Budinger GR; Mutlu GM; Eisenbart J; Fuller AC; Bellmeyer AA; Baker CM; Wilson M; Ridge K; Barrett TA; Lee VY; Chandel NS. 2006. Proapoptotic Bid is required for pulmonary fibrosis. Proc Natl Acad Sci U S A 103(12):4604-9. [PubMed: 16537427]  [MGI Ref ID J:107667]

Camacho IA; Singh N; Hegde VL; Nagarkatti M; Nagarkatti PS. 2005. Treatment of mice with 2,3,7,8-tetrachlorodibenzo-p-dioxin leads to aryl hydrocarbon receptor-dependent nuclear translocation of NF-kappaB and expression of Fas ligand in thymic stromal cells and consequent apoptosis in T cells. J Immunol 175(1):90-103. [PubMed: 15972635]  [MGI Ref ID J:100624]

Chang KC; Unsinger J; Davis CG; Schwulst SJ; Muenzer JT; Strasser A; Hotchkiss RS. 2007. Multiple triggers of cell death in sepsis: death receptor and mitochondrial-mediated apoptosis. FASEB J 21(3):708-19. [PubMed: 17307841]  [MGI Ref ID J:134855]

Chen X; Ding WX; Ni HM; Gao W; Shi YH; Gambotto AA; Fan J; Beg AA; Yin XM. 2007. Bid-independent mitochondrial activation in tumor necrosis factor alpha-induced apoptosis and liver injury. Mol Cell Biol 27(2):541-53. [PubMed: 17101783]  [MGI Ref ID J:117693]

Croker BA; O'Donnell JA; Nowell CJ; Metcalf D; Dewson G; Campbell KJ; Rogers KL; Hu Y; Smyth GK; Zhang JG; White M; Lackovic K; Cengia LH; O'Reilly LA; Bouillet P; Cory S; Strasser A; Roberts AW. 2011. Fas-mediated neutrophil apoptosis is accelerated by Bid, Bak, and Bax and inhibited by Bcl-2 and Mcl-1. Proc Natl Acad Sci U S A 108(32):13135-40. [PubMed: 21768356]  [MGI Ref ID J:176025]

D'Orsi B; Bonner H; Tuffy LP; Dussmann H; Woods I; Courtney MJ; Ward MW; Prehn JH. 2012. Calpains are downstream effectors of bax-dependent excitotoxic apoptosis. J Neurosci 32(5):1847-58. [PubMed: 22302823]  [MGI Ref ID J:181340]

Danthi P; Pruijssers AJ; Berger AK; Holm GH; Zinkel SS; Dermody TS. 2010. Bid regulates the pathogenesis of neurotropic reovirus. PLoS Pathog 6:e1000980. [PubMed: 20617182]  [MGI Ref ID J:162998]

Dent P; Han SI; Mitchell C; Studer E; Yacoub A; Grandis J; Grant S; Krystal GW; Hylemon PB. 2005. Inhibition of insulin/IGF-1 receptor signaling enhances bile acid toxicity in primary hepatocytes. Biochem Pharmacol 70(11):1685-96. [PubMed: 16207485]  [MGI Ref ID J:104645]

Ekoff M; Kaufmann T; Engstrom M; Motoyama N; Villunger A; Jonsson JI; Strasser A; Nilsson G. 2007. The BH3-only protein Puma plays an essential role in cytokine deprivation induced apoptosis of mast cells. Blood 110(9):3209-17. [PubMed: 17634411]  [MGI Ref ID J:149129]

Estella E; McKenzie MD; Catterall T; Sutton VR; Bird PI; Trapani JA; Kay TW; Thomas HE. 2006. Granzyme B-mediated death of pancreatic beta-cells requires the proapoptotic BH3-only molecule bid. Diabetes 55(8):2212-9. [PubMed: 16873683]  [MGI Ref ID J:116520]

Fischer SF; Belz GT; Strasser A. 2008. BH3-only protein Puma contributes to death of antigen-specific T cells during shutdown of an immune response to acute viral infection. Proc Natl Acad Sci U S A 105(8):3035-40. [PubMed: 18287039]  [MGI Ref ID J:132819]

Fischer SF; Bouillet P; O'Donnell K; Light A; Tarlinton DM; Strasser A. 2007. Proapoptotic BH3-only protein Bim is essential for developmentally programmed death of germinal center-derived memory B cells and antibody-forming cells. Blood 110(12):3978-84. [PubMed: 17720882]  [MGI Ref ID J:149102]

Geering B; Gurzeler U; Federzoni E; Kaufmann T; Simon HU. 2011. A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils. Blood 117(22):5953-62. [PubMed: 21478427]  [MGI Ref ID J:174714]

Gianino S; Grider JR; Cresswell J; Enomoto H; Heuckeroth RO. 2003. GDNF availability determines enteric neuron number by controlling precursor proliferation. Development 130(10):2187-98. [PubMed: 12668632]  [MGI Ref ID J:82456]

Gimenez-Cassina A; Martinez-Francois JR; Fisher JK; Szlyk B; Polak K; Wiwczar J; Tanner GR; Lutas A; Yellen G; Danial NN. 2012. BAD-dependent regulation of fuel metabolism and K(ATP) channel activity confers resistance to epileptic seizures. Neuron 74(4):719-30. [PubMed: 22632729]  [MGI Ref ID J:188361]

Hodge DL; Yang J; Buschman MD; Schaughency PM; Dang H; Bere W; Yang Y; Savan R; Subleski JJ; Yin XM; Loughran TP Jr; Young HA. 2009. Interleukin-15 enhances proteasomal degradation of bid in normal lymphocytes: implications for large granular lymphocyte leukemias. Cancer Res 69(9):3986-94. [PubMed: 19366803]  [MGI Ref ID J:148275]

Houseweart MK; Vilaythong A; Yin XM; Turk B; Noebels JL; Myers RM. 2003. Apoptosis caused by cathepsins does not require Bid signaling in an in vivo model of progressive myoclonus epilepsy (EPM1). Cell Death Differ 10(12):1329-35. [PubMed: 12934064]  [MGI Ref ID J:115694]

Jabbour AM; Heraud JE; Daunt CP; Kaufmann T; Sandow J; O'Reilly LA; Callus BA; Lopez A; Strasser A; Vaux DL; Ekert PG. 2009. Puma indirectly activates Bax to cause apoptosis in the absence of Bid or Bim. Cell Death Differ 16(4):555-63. [PubMed: 19079139]  [MGI Ref ID J:158076]

Jacobs SB; Basak S; Murray JI; Pathak N; Attardi LD. 2007. Siva is an apoptosis-selective p53 target gene important for neuronal cell death. Cell Death Differ 14(7):1374-85. [PubMed: 17464332]  [MGI Ref ID J:139268]

Jost PJ; Grabow S; Gray D; McKenzie MD; Nachbur U; Huang DC; Bouillet P; Thomas HE; Borner C; Silke J; Strasser A; Kaufmann T. 2009. XIAP discriminates between type I and type II FAS-induced apoptosis. Nature 460(7258):1035-9. [PubMed: 19626005]  [MGI Ref ID J:151786]

Kang HR; Cho SJ; Lee CG; Homer RJ; Elias JA. 2007. Transforming growth factor (TGF)-beta1 stimulates pulmonary fibrosis and inflammation via a Bax-dependent, bid-activated pathway that involves matrix metalloproteinase-12. J Biol Chem 282(10):7723-32. [PubMed: 17209037]  [MGI Ref ID J:120886]

Kang SJ; Wang S; Kuida K; Yuan J. 2002. Distinct downstream pathways of caspase-11 in regulating apoptosis and cytokine maturation during septic shock response. Cell Death Differ 9(10):1115-25. [PubMed: 12232800]  [MGI Ref ID J:115554]

Kaufmann T; Gugasyan R; Gerondakis S; Dixit VM; Strasser A. 2007. Loss of the BH3-only protein Bid does not rescue RelA-deficient embryos from TNF-R1-mediated fatal hepatocyte destruction. Cell Death Differ 14(3):637-9. [PubMed: 17096024]  [MGI Ref ID J:132334]

Kaufmann T; Jost PJ; Pellegrini M; Puthalakath H; Gugasyan R; Gerondakis S; Cretney E; Smyth MJ; Silke J; Hakem R; Bouillet P; Mak TW; Dixit VM; Strasser A. 2009. Fatal hepatitis mediated by tumor necrosis factor TNFalpha requires caspase-8 and involves the BH3-only proteins Bid and Bim. Immunity 30(1):56-66. [PubMed: 19119023]  [MGI Ref ID J:143730]

Kaufmann T; Tai L; Ekert PG; Huang DC; Norris F; Lindemann RK; Johnstone RW; Dixit VM; Strasser A. 2007. The BH3-only protein bid is dispensable for DNA damage- and replicative stress-induced apoptosis or cell-cycle arrest. Cell 129(2):423-33. [PubMed: 17448999]  [MGI Ref ID J:158191]

Kodama T; Takehara T; Hikita H; Shimizu S; Shigekawa M; Li W; Miyagi T; Hosui A; Tatsumi T; Ishida H; Kanto T; Hiramatsu N; Yin XM; Hayashi N. 2011. BH3-only Activator Proteins Bid and Bim Are Dispensable for Bak/Bax-dependent Thrombocyte Apoptosis Induced by Bcl-xL Deficiency: MOLECULAR REQUISITES FOR THE MITOCHONDRIAL PATHWAY TO APOPTOSIS IN PLATELETS. J Biol Chem 286(16):13905-13. [PubMed: 21367852]  [MGI Ref ID J:171123]

Leonard JR; D'Sa C; Cahn BR; Korsmeyer SJ; Roth KA. 2001. Bid regulation of neuronal apoptosis. Brain Res Dev Brain Res 128(2):187-90. [PubMed: 11412905]  [MGI Ref ID J:71150]

Li B; Cao CP; Mao GP. 2005. Effect of proapoptosis protein on hepatocarcinogenesis. Chin J Dig Dis 6(2):93-7. [PubMed: 15904428]  [MGI Ref ID J:101115]

Maret M; Ruffie C; Letuve S; Phelep A; Thibaudeau O; Marchal J; Pretolani M; Druilhe A. 2009. A role for Bid in eosinophil apoptosis and in allergic airway reaction. J Immunol 182(9):5740-7. [PubMed: 19380821]  [MGI Ref ID J:147707]

Maryanovich M; Oberkovitz G; Niv H; Vorobiyov L; Zaltsman Y; Brenner O; Lapidot T; Jung S; Gross A. 2012. The ATM-BID pathway regulates quiescence and survival of haematopoietic stem cells. Nat Cell Biol 14(5):535-41. [PubMed: 22446738]  [MGI Ref ID J:185449]

McKenzie MD; Carrington EM; Kaufmann T; Strasser A; Huang DC; Kay TW; Allison J; Thomas HE. 2008. Proapoptotic BH3-only protein Bid is essential for death receptor-induced apoptosis of pancreatic beta-cells. Diabetes 57(5):1284-92. [PubMed: 18252892]  [MGI Ref ID J:135326]

Nalapareddy P; Schungel S; Hong JY; Manns MP; Jaeschke H; Vogel A. 2009. The BH3-only protein bid does not mediate death-receptor-induced liver injury in obstructive cholestasis. Am J Pathol 175(3):1077-85. [PubMed: 19661444]  [MGI Ref ID J:152902]

Ness JM; Harvey CA; Strasser A; Bouillet P; Klocke BJ; Roth KA. 2006. Selective involvement of BH3-only Bcl-2 family members Bim and Bad in neonatal hypoxia-ischemia. Brain Res 1099(1):150-9. [PubMed: 16780816]  [MGI Ref ID J:111236]

Ni HM; Chen X; Shi YH; Liao Y; Beg AA; Fan J; Yin XM. 2009. Genetic delineation of the pathways mediated by bid and JNK in tumor necrosis factor-alpha-induced liver injury in adult and embryonic mice. J Biol Chem 284(7):4373-82. [PubMed: 19060338]  [MGI Ref ID J:147600]

Pardo J; Urban C; Galvez EM; Ekert PG; Muller U; Kwon-Chung J; Lobigs M; Mullbacher A; Wallich R; Borner C; Simon MM. 2006. The mitochondrial protein Bak is pivotal for gliotoxin-induced apoptosis and a critical host factor of Aspergillus fumigatus virulence in mice. J Cell Biol 174(4):509-19. [PubMed: 16893972]  [MGI Ref ID J:112620]

Plesnila N; Zinkel S; Amin-Hanjani S; Qiu J; Korsmeyer SJ; Moskowitz MA. 2002. Function of BID -- a molecule of the bcl-2 family -- in ischemic cell death in the brain. Eur Surg Res 34(1-2):37-41. [PubMed: 11867899]  [MGI Ref ID J:112420]

Plesnila N; Zinkel S; Le DA; Amin-Hanjani S; Wu Y; Qiu J; Chiarugi A; Thomas SS; Kohane DS; Korsmeyer SJ; Moskowitz MA. 2001. BID mediates neuronal cell death after oxygen/ glucose deprivation and focal cerebral ischemia. Proc Natl Acad Sci U S A 98(26):15318-23. [PubMed: 11742085]  [MGI Ref ID J:73477]

Pradhan S; Genebriera J; Denning WL; Felix K; Elmets CA; Timares L. 2006. CD4 T cell-induced, bid-dependent apoptosis of cutaneous dendritic cells regulates T cell expansion and immune responses. J Immunol 177(9):5956-67. [PubMed: 17056520]  [MGI Ref ID J:140532]

Pradhan S; Kim HK; Thrash CJ; Cox MA; Mantena SK; Wu JH; Athar M; Katiyar SK; Elmets CA; Timares L. 2008. A critical role for the proapoptotic protein bid in ultraviolet-induced immune suppression and cutaneous apoptosis. J Immunol 181(5):3077-88. [PubMed: 18713978]  [MGI Ref ID J:138961]

Qiu W; Wu B; Wang X; Buchanan ME; Regueiro MD; Hartman DJ; Schoen RE; Yu J; Zhang L. 2011. PUMA-mediated intestinal epithelial apoptosis contributes to ulcerative colitis in humans and mice. J Clin Invest 121(5):1722-32. [PubMed: 21490394]  [MGI Ref ID J:173951]

Ren D; Tu HC; Kim H; Wang GX; Bean GR; Takeuchi O; Jeffers JR; Zambetti GP; Hsieh JJ; Cheng EH. 2010. BID, BIM, and PUMA are essential for activation of the BAX- and BAK-dependent cell death program. Science 330(6009):1390-3. [PubMed: 21127253]  [MGI Ref ID J:167307]

Shen H; Yu H; Liang PH; Xufeng R; Song Y; Hu X; Chen X; Yin XM; Cheng T. 2011. Bid is a positive regulator for donor-derived lymphoid cell regeneration in gamma-irradiated recipients. Exp Hematol 39(9):947-957.e1. [PubMed: 21703985]  [MGI Ref ID J:186806]

Takeda K; Kojima Y; Ikejima K; Harada K; Yamashina S; Okumura K; Aoyama T; Frese S; Ikeda H; Haynes NM; Cretney E; Yagita H; Sueyoshi N; Sato N; Nakanuma Y; Smyth MJ; Okumura K. 2008. Death receptor 5 mediated-apoptosis contributes to cholestatic liver disease. Proc Natl Acad Sci U S A 105(31):10895-900. [PubMed: 18667695]  [MGI Ref ID J:139942]

Upton JP; Austgen K; Nishino M; Coakley KM; Hagen A; Han D; Papa FR; Oakes SA. 2008. Caspase-2 cleavage of BID is a critical apoptotic signal downstream of endoplasmic reticulum stress. Mol Cell Biol 28(12):3943-51. [PubMed: 18426910]  [MGI Ref ID J:137299]

Vince JE; Wong WW; Gentle I; Lawlor KE; Allam R; O'Reilly L; Mason K; Gross O; Ma S; Guarda G; Anderton H; Castillo R; Hacker G; Silke J; Tschopp J. 2012. Inhibitor of Apoptosis Proteins Limit RIP3 Kinase-Dependent Interleukin-1 Activation. Immunity 36(2):215-27. [PubMed: 22365665]  [MGI Ref ID J:181625]

Wang HL; Akinci IO; Baker CM; Urich D; Bellmeyer A; Jain M; Chandel NS; Mutlu GM; Budinger GR. 2007. The intrinsic apoptotic pathway is required for lipopolysaccharide-induced lung endothelial cell death. J Immunol 179(3):1834-41. [PubMed: 17641050]  [MGI Ref ID J:149939]

Wang X; Ryter SW; Dai C; Tang ZL; Watkins SC; Yin XM; Song R; Choi AM. 2003. Necrotic cell death in response to oxidant stress involves the activation of the apoptogenic caspase-8/bid pathway. J Biol Chem 278(31):29184-91. [PubMed: 12754217]  [MGI Ref ID J:120650]

Waterhouse NJ; Sedelies KA; Browne KA; Wowk ME; Newbold A; Sutton VR; Clarke CJ; Oliaro J; Lindemann RK; Bird PI; Johnstone RW; Trapani JA. 2005. A central role for Bid in granzyme B-induced apoptosis. J Biol Chem 280(6):4476-82. [PubMed: 15574417]  [MGI Ref ID J:96908]

Wei Q; Yin XM; Wang MH; Dong Z. 2006. Bid deficiency ameliorates ischemic renal failure and delays animal death in C57BL/6 mice. Am J Physiol Renal Physiol 290(1):F35-42. [PubMed: 16106037]  [MGI Ref ID J:104086]

Yin XM; Luo Y; Cao G; Bai L; Pei W; Kuharsky DK; Chen J. 2002. Bid-mediated mitochondrial pathway is critical to ischemic neuronal apoptosis and focal cerebral ischemia. J Biol Chem 277(44):42074-81. [PubMed: 12200426]  [MGI Ref ID J:79875]

Zhao Y; Li S; Childs EE; Kuharsky DK; Yin XM. 2001. Activation of pro-death bcl-2 family proteins and mitochondria apoptosis pathway in tumor necrosis factor-alpha -induced liver injury. J Biol Chem 276(29):27432-40. [PubMed: 11369777]  [MGI Ref ID J:70549]

Zinkel SS; Ong CC; Ferguson DO; Iwasaki H; Akashi K; Bronson RT; Kutok JL; Alt FW; Korsmeyer SJ. 2003. Proapoptotic BID is required for myeloid homeostasis and tumor suppression. Genes Dev 17(2):229-39. [PubMed: 12533511]  [MGI Ref ID J:81329]

Ziporen L; Donin N; Shmushkovich T; Gross A; Fishelson Z. 2009. Programmed necrotic cell death induced by complement involves a Bid-dependent pathway. J Immunol 182(1):515-21. [PubMed: 19109183]  [MGI Ref ID J:142888]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Mating System	Homozygote x Homozygote         (Female x Male)   17-NOV-09

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.