| |||||||||||||||
| These ROSA26-Syn119 mice allow cre-inducible expression of the Syn119 C-terminally truncated mutation of human alpha-synuclein (human α-Syn119 or SynCT119; associated with familial Parkinson's disease), and may be useful for studying the progressive dopaminergic neurodegeneration of Parkinson's disease and other synucleinopathies, Lewy bodies, and synaptic plasticity. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x Heterozygote (Female x Male) 08-AUG-09 Species laboratory mouse Generation F?+N1F1 (12-NOV-09) Donating Investigator Darren Moore, Ecole Polytechnique Federale de Lausanne Donating Investigator Ted Dawson, Johns Hopkins Univ School of Medicine Description
Homozygous ROSA26-Syn119 mice are viable and fertile, with the familial Parkinson's disease-associated Syn119 C-terminal truncation of human alpha-synuclein (human α-Syn119 or SynCT119) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of human α-Syn119 is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no human α-Syn119 protein is observed in brain regions). When bred to cre expressing mice, the STOP sequence is deleted in the tissues of offspring where Cre recombinase is present; resulting in human α-Syn119 expression. In particular, Stock No. 008601 B6.Cg-Tg(Th-cre)1Tmd/J may be useful for this application. These ROSA26-Syn119 mice allow inducible expression of a human mutation associated with familial Parkinson's disease and may be useful for studying the progressive dopaminergic neurodegeneration of Parkinson's disease and other synucleinopathies, Lewy bodies, and synaptic plasticity.When bred to a strain expressing Cre recombinase in central catecholaminergic neurons (see Stock No. 008601 for example), this mutant mouse strain may be useful in studies of Parkinson's disease.
Development
A targeting vector was designed with an SV40 splice acceptor, a loxP-flanked STOP cassette (PGK-neo and triple SV40 transcriptional termination sequence), a cDNA encoding the 119 amino acid C-terminal truncation of human alpha-synuclein associated with familial Parkinson's disease (Syn119 or SynCT119; encoding amino acids 1-119 of the full length protein), and a bovine polyadenylation signal. This construct was inserted into the Gt(ROSA)26Sor locus via electroporation into into 129/SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6J mice to generate the mutant colony. ROSA26-Syn119 mice were bred to C57BL/6 for 2-3 generations and then bred together to make homozygous prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (64 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Gt(ROSA)26Sortm2(SNCA*119)Djmo/Gt(ROSA)26Sortm2(SNCA*119)Djmo
involves: 129 * C57BL/6J (conditional)
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:147331)
- mice are viable and fertile
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Gt(ROSA)26Sortm2(SNCA*119)Djmo/Gt(ROSA)26Sor+ Tg(Nes-cre)1Kln/0
involves: 129 * C57BL/6 * SJL (conditional)
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:150777)
Gt(ROSA)26Sortm2(SNCA*119)Djmo/Gt(ROSA)26Sortm2(SNCA*119)Djmo Tg(Th-cre)1Tmd/0
involves: 129 * C57BL/6 (conditional)
- nervous system phenotype
- decreased dopamine level (MGI Ref ID J:150777)
- 32% reduction in levels of striatal dopamine observed in 10-11 month old mice
- homeostasis/metabolism phenotype
- abnormal serotonin level (MGI Ref ID J:150777)
- increased levels of 5-HT observed in prefrontal cortex in 10-11 month old mice
- increased circulating noradrenaline level (MGI Ref ID J:150777)
- increased levels of norepinephrine observed in striatum in 10-11 month old mice
Gt(ROSA)26Sortm2(SNCA*119)Djmo/Gt(ROSA)26Sortm2(SNCA*119)Djmo Tg(Th-cre)1Tmd/0
involves: 129 * C57BL/6 * SJL (conditional)
- homeostasis/metabolism phenotype
- abnormal serotonin level (MGI Ref ID J:150777)
- 5-HT levels are elevated in the prefrontal cortex at 10-11 months of age compared to controls
- nervous system phenotype
- abnormal neurotransmitter level (MGI Ref ID J:150777)
- norepinephrine levels in the striatum are elevated at 10-11 months of age compared to controls
- decreased dopamine level (MGI Ref ID J:150777)
- there are significant reductions in the levels of striatal dopamine (~32%), dihydroxyphenylacetic acid (~46%) and homovanillic acid (~33%) compared to controls at 10-11 months of age
This mouse can be used to support research in many areas including:
Neurobiology Research
Ataxia (Movement) Defects
Cre-lox System
loxP-flanked Sequences
Neurodegeneration
Neurotransmitter Receptor and Synaptic Vesicle Defects
Parkinson's Disease
synuclein mutants
Research Tools
Cre-lox System
loxP-flanked Sequences
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Neurobiology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm2(SNCA*119)Djmo | ||
|---|---|---|---|
| Allele Name | Targeted mutation 2, Darren Moore | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | ROSA26-Syn119; ROSA26-SynCT119Tg; ROSA26-a-synuclein C-terminal truncation variant (Syn119); | ||
| Mutation Made By | Darren Moore, Ecole Polytechnique Federale de Lausanne | ||
| Strain of Origin | 129 | ||
| ES Cell Line Name | Other (see notes) | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| General Note | Authors state that 129/SvJ-derived embryonic stem cells were used. | ||
| Molecular Note | A targeting vector was designed with an SV40 splice acceptor, a loxP-flanked STOP cassette (PGK-neo and triple SV40 transcriptional termination sequence), a cDNA encoding the 119 amino acid C-terminal truncation of human alpha-synuclein associated with familial Parkinson's disease (Syn119 or SynCT119; encoding amino acids 1-119 of the full length protein), and a bovine polyadenylation signal. [MGI Ref ID J:147331] [MGI Ref ID J:150777] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Daher JP; Ying M; Banerjee R; McDonald RS; Dumas Hahn M; Yang L; Beal MF; Thomas B; Dawson VL; Dawson TM; Moore DJ. 2009. Conditional transgenic mice expressing C-terminally truncated human alpha-synuclein (alphaSyn119) exhibit reduced striatal dopamine without loss of nigrostriatal pathway dopaminergic neurons. Mol Neurodegener 4(1):34. [PubMed: 19630976] [MGI Ref ID J:150777]
Additional References
Gt(ROSA)26Sortm2(SNCA*119)Djmo relatedMoore D. 2009. Generation of a conditional knockin allele expresing a truncated human alpha-synuclein (SNCA) MGI Direct Data Submission :. [MGI Ref ID J:147331]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Heterozygote x Heterozygote (Female x Male) 08-AUG-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Gt(ROSA)26Sortm2(SNCA*119)Djmo $300.70 Female or Male Homozygous for Gt(ROSA)26Sortm2(SNCA*119)Djmo
Pairs /Price (US dollars $) Pair Genotype $487.00 Heterozygous for Gt(ROSA)26Sortm2(SNCA*119)Djmo x Heterozygous for Gt(ROSA)26Sortm2(SNCA*119)Djmo
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Gt(ROSA)26Sortm2(SNCA*119)Djmo $391.00 Female or Male Homozygous for Gt(ROSA)26Sortm2(SNCA*119)Djmo
Pairs /Price (US dollars $) Pair Genotype $633.10 Heterozygous for Gt(ROSA)26Sortm2(SNCA*119)Djmo x Heterozygous for Gt(ROSA)26Sortm2(SNCA*119)Djmo
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.