Strain Name:

B6.129-Gria1tm1Rlh/J

Stock Number:

008892

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Cryopreserved - Ready for recovery

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These mice carry S831A and S845A phosphorylation site mutations of GlurR1 (Gria1, glutamate receptor, ionotropic, AMPA1 (alpha 1)). Mutant mice exhibit deficits in synaptic plasticity and deterioration of learning/memory performance.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Richard L Huganir,   Johns Hopkins University; HHMI

Description
Mice that are homozygous for the S831A and S845A phosphorylation site mutations of GlurR1 are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Phosphorylation of the targeted amino acids is not detected by Western blot analysis of the brain isolated from homozygous animals. Mutant mice exhibit deficits in synaptic plasticity and deterioration of learning/memory performance.

Development
A targeting vector containing a loxP site-flanked neomycin resistance cassette was inserted into intron 10 with alanine mutation in S831 and S845 sites. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. After germline transmission, mice were bred to CMV-Cre transgenic mice to delete neomycin resistance cassette. The CMV-Cre transgene was bred out from the line and mutant mice carrying correct mutation were backcrossed to C57BL/6J more than 10 times.

Related Strains

Strains carrying other alleles of Gria1
012614   B6.129-Gria1tm2Rlh/J
012612   B6.129-Gria1tm5Rlh/J
012613   B6.129-Gria1tm6Rlh/J
019011   B6N.129-Gria1tm1Rsp/J
019012   B6N.129-Gria1tm2Rsp/J
View Strains carrying other alleles of Gria1     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Gria1tm1Rlh/Gria1tm1Rlh

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • behavior/neurological phenotype
  • abnormal associative learning
    • mice fail to increase pokes of their nose into the prefered nose port upon a conditioned auditory cue while wild-type mice given the same training significantly increase the number of nose pokes by 5-fold   (MGI Ref ID J:135196)
    • the discrimination ratio between the conditioned and unconditioned noseport fails to be significantly different from chance   (MGI Ref ID J:135196)
    • the deficit in discriminating responding to the auditor cue is evident at the beginning of the test (i.e. does not result from differences in extinction rates)   (MGI Ref ID J:135196)
    • mice have no defects in normal food responses or overall locomotor activity   (MGI Ref ID J:135196)
  • abnormal long term spatial reference memory
    • mutants exhibit a selective impairment in the retention of new learning at delays longer than a few hours, however learning is normal in the standard water maze task   (MGI Ref ID J:82443)
  • nervous system phenotype
  • absent long term depression   (MGI Ref ID J:82443)
  • reduced long term potentiation
    • LTP induced by theta burst stimulation is greatly reduced in adult, but not young, mutants   (MGI Ref ID J:82443)
    • LTP induced by a conventional 1 Hz protocol is absent in young (P21-P28) mutants   (MGI Ref ID J:82443)
    • LTP induced by pairing depolarizing current injection with low-frequency stimulation is severely reduced   (MGI Ref ID J:82443)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
Receptor Defects
      glutamate receptor: ionotropic

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Gria1tm1Rlh
Allele Name targeted mutation 1, Richard L Huganir
Allele Type Targeted (knock-in)
Common Name(s) GR1AA;
Mutation Made By Richard Huganir,   Johns Hopkins University; HHMI
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Promoter Gria1, glutamate receptor, ionotropic, AMPA1 (alpha 1), mouse, laboratory
Molecular Note Alanine amino acid substitutions were introduced to phosphorylation sites serine 831 (S831A) and serine 845 (S845A) in exon 17 of the gene. A floxed neomycin resistance cassette was inserted upstream of exon 17 for positive selection. Mutant animals werecrossed to cre deleter strain Tg(CMV-cre)1Nagy to removed the floxed neo cassette, leaving behind the 2 amino acid substitutions. The targeted mutation was confirmed using immunoblot with phosphorylation site-specific antibodies. [MGI Ref ID J:82443]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lee HK; Takamiya K; Han JS; Man H; Kim CH; Rumbaugh G; Yu S; Ding L; He C; Petralia RS; Wenthold RJ; Gallagher M; Huganir RL. 2003. Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory. Cell 112(5):631-43. [PubMed: 12628184]  [MGI Ref ID J:82443]

Additional References

Gria1tm1Rlh related

Crombag HS; Sutton JM; Takamiya K; Holland PC; Gallagher M; Huganir RL. 2008. A role for alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid GluR1 phosphorylation in the modulatory effects of appetitive reward cues on goal-directed behavior. Eur J Neurosci 27(12):3284-91. [PubMed: 18598267]  [MGI Ref ID J:137440]

Crombag HS; Sutton JM; Takamiya K; Lee HK; Holland PC; Gallagher M; Huganir RL. 2008. A necessary role for GluR1 serine 831 phosphorylation in appetitive incentive learning. Behav Brain Res 191(2):178-83. [PubMed: 18455244]  [MGI Ref ID J:135196]

Goel A; Xu LW; Snyder KP; Song L; Goenaga-Vazquez Y; Megill A; Takamiya K; Huganir RL; Lee HK. 2011. Phosphorylation of AMPA receptors is required for sensory deprivation-induced homeostatic synaptic plasticity. PLoS One 6(3):e18264. [PubMed: 21483826]  [MGI Ref ID J:171435]

Hu H; Real E; Takamiya K; Kang MG; Ledoux J; Huganir RL; Malinow R. 2007. Emotion enhances learning via norepinephrine regulation of AMPA-receptor trafficking. Cell 131(1):160-73. [PubMed: 17923095]  [MGI Ref ID J:141480]

Kim CH; Takamiya K; Petralia RS; Sattler R; Yu S; Zhou W; Kalb R; Wenthold R; Huganir R. 2005. Persistent hippocampal CA1 LTP in mice lacking the C-terminal PDZ ligand of GluR1. Nat Neurosci 8(8):985-7. [PubMed: 16007085]  [MGI Ref ID J:101578]

Rakhade SN; Fitzgerald EF; Klein PM; Zhou C; Sun H; Hunganir RL; Jensen FE. 2012. Glutamate receptor 1 phosphorylation at serine 831 and 845 modulates seizure susceptibility and hippocampal hyperexcitability after early life seizures. J Neurosci 32(49):17800-12. [PubMed: 23223299]  [MGI Ref ID J:193191]

Seol GH; Ziburkus J; Huang S; Song L; Kim IT; Takamiya K; Huganir RL; Lee HK; Kirkwood A. 2007. Neuromodulators control the polarity of spike-timing-dependent synaptic plasticity. Neuron 55(6):919-29. [PubMed: 17880895]  [MGI Ref ID J:132105]

Svenningsson P; Bateup H; Qi H; Takamiya K; Huganir RL; Spedding M; Roth BL; McEwen BS; Greengard P. 2007. Involvement of AMPA receptor phosphorylation in antidepressant actions with special reference to tianeptine. Eur J Neurosci 26(12):3509-17. [PubMed: 18088278]  [MGI Ref ID J:130859]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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