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| A loxP-flanked puromycin resistance/STOP cassette followed by a fusion of firefly luciferase and synthetic antigen peptide SIYRYYGL were introduced to the Gt(ROSA)26Sor (gene trap ROSA 26, Philippe Soriano) locus. After Cre activity, the recombined locus has high expression of Luciferase-SIY fusion protein. This mutant mouse strain may be useful in studies of immune response to a self-antigen or over-expressed tumor-associated antigen when used in combination with tumor-prone models. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote (Female x Male) 16-JUN-09 Species laboratory mouse Generation N13+F2 (18-NOV-09) Donating Investigator Tyler Jacks, Massachusetts Institute of Technology Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In the absence of Cre, there is low level expression of Luciferase-SIY H2b antigen expression. After Cre activity, the recombined locus has high expression of Luciferase-SIY fusion protein. This mutant mouse strain may be useful in studies of immune response to a self-antigen or over-expressed tumor-associated antigen when used in combination with tumor-prone models. This mouse is also useful as a reporter for Cre activity.Development
A loxP-flanked puromycin resistance/STOP cassette followed by a fusion of firefly luciferase and synthetic antigen peptide SIYRYYGL were introduced to the targeted gene using 129S4/SvJae-derived J1 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for 13 generations by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm2Tyj allele
009043 129S-Gt(ROSA)26Sortm2Tyj/J View Strains carrying Gt(ROSA)26Sortm2Tyj (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cancer Research
Research Tools
Cancer Research
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm2Tyj | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Tyler Jacks | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R26LSL-LSIY; | ||
| Mutation Made By | Tyler Jacks, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A cassette containing a floxed STOP followed by a luciferase gene and sequence encoding the antigen SIYRYYGL (SIY) was inserted into the locus. Cre-mediated recombination is necessary to allow for expression of SIYRYYGL. [MGI Ref ID J:141383] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm2Tyj, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Cheung AF; Dupage MJ; Dong HK; Chen J; Jacks T. 2008. Regulated expression of a tumor-associated antigen reveals multiple levels of T-cell tolerance in a mouse model of lung cancer. Cancer Res 68(22):9459-68. [PubMed: 19010921] [MGI Ref ID J:141383]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred. Mating System Heterozygote x Heterozygote (Female x Male) 16-JUN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Gt(ROSA)26Sortm2Tyj $300.70 Female or Male Homozygous for Gt(ROSA)26Sortm2Tyj
Pairs /Price (US dollars $) Pair Genotype $487.00 Heterozygous for Gt(ROSA)26Sortm2Tyj x Heterozygous for Gt(ROSA)26Sortm2Tyj
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Gt(ROSA)26Sortm2Tyj $391.00 Female or Male Homozygous for Gt(ROSA)26Sortm2Tyj
Pairs /Price (US dollars $) Pair Genotype $633.10 Heterozygous for Gt(ROSA)26Sortm2Tyj x Heterozygous for Gt(ROSA)26Sortm2Tyj
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| phone: | 207-288-6470 |
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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