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| These Ednrbflex3 mutant mice harbor a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the Ednrb (endothelin receptor type B or ET-B receptor) gene and may be useful in generating conditional mutations for studying the role of Ednrb in development of melanocytes, development of neurons and glia of the enteric nervous system, neural crest-derived cells, mesenchymal-derived smooth muscle cells, vasodilation, mitogen signaling and cancer, and human Hirschsprung's disease (HSCR). | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+F2p
Generation DefinitionsDonating Investigator Noah Druckenbrod, University Of Wisconsin Description
Mice heterozygous for this Ednrbflex3 allele are viable and fertile, with a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the Ednrb (endothelin receptor type B or ET-B receptor) gene. When bred to mice that express Cre recombinase, the resulting offspring can have one of three resulting genotypes in the cre-expressing tissue(s); only the neo selection cassette deleted, only exon 3 deleted, or both the neo selection cassette and exon 3 deleted. The two latter genotypes are expected to result in a frameshifted transcript that is reported to confer the null phenotype. These mutant mice may be useful in generating conditional mutations for studying the role of Ednrb in development of melanocytes, development of neurons and glia of the enteric nervous system, neural crest-derived cells, mesenchymal-derived smooth muscle cells, vasodilation, mitogen signaling and cancer, and human Hirschsprung's disease (HSCR).When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of neural crest development.
Development
A targeting vector was designed to place a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the targeted gene. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6 females. The resulting Ednrbflex3 mutant mice were then bred to Wnt1-Cre mice (reported to be C57BL/6J genetic background (although they were white); see Stock No. 007807). Double mutant mice were bred together or to Ednrbflex3 single mutant mice for approximately four years prior to arrival at The Jackson Laboratory. Upon arrival, Ednrbflex3 mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to confirm the Wnt1-Cre transgene was removed.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ednrb
000577 B6 x STOCK a Oca2p Hps5ru2 Ednrbs/J 011080 B6;129-Ednrbtm1.1Nat/J 003295 B6;129-Ednrbtm1Ywa/J 000674 I/LnJ 003720 JF1/Ms 000676 LP/J 000308 SSL/LeJ 004711 STOCK Ednrbs-52Pub 000275 V/LeJ View Strains carrying other alleles of Ednrb (9 strains)
010633 B6(Cg)-Gt(ROSA)26Sortm1(CAG-taulacZ)Bene/J 012247 B6.129-Aldh1a1tm1Gdu/J 012838 B6.129S4-Tnfrsf4tm1Nik/J 006382 B6;129-Casktm1Sud/J 016857 B6;129-Itga7tm1Burk/J 010711 C57BL/6-Ptrh2tm1Eruo/J 002356 C57BL/6J-Tg(pPGKneobpA)3Ems/J 012940 STOCK Snta1tm1Scf/J 003208 STOCK Tg(DR4)1Jae/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Abcd Syndrome; ABCDS (EDNRB)
Hirschsprung Disease, Susceptibility to, 2; HSCR2 (EDNRB)
Waardenburg Syndrome, Type 4A; WS4A (EDNRB)
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Ednrbtm1Nrd/Ednrbtm1Nrd Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+ Tg(Wnt1-cre)11Rth/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA/J (conditional)
- mortality/aging
- premature death
- mice die 5 weeks after birth (MGI Ref ID J:140320)
- growth/size phenotype
- distended abdomen (MGI Ref ID J:140320)
- slow postnatal weight gain
- after P25 (MGI Ref ID J:140320)
- digestive/alimentary phenotype
- aganglionic megacolon (MGI Ref ID J:140320)
- pigmentation phenotype
- white spotting
- mice lack coat pigment in the trunk (MGI Ref ID J:140320)
- embryogenesis phenotype
- abnormal neural crest cell migration
- enteric neural crest cells fail to reach the anus (MGI Ref ID J:140320)
- integument phenotype
- white spotting
- mice lack coat pigment in the trunk (MGI Ref ID J:140320)
- cellular phenotype
- abnormal neural crest cell migration
- enteric neural crest cells fail to reach the anus (MGI Ref ID J:140320)
This mouse can be used to support research in many areas including:
neo relatedDevelopmental Biology Research
Neural Crest Defects
Mouse/Human Gene Homologs
Hirschsprung disease
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
Research Tools
Cancer Research
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Internal/Organ Research
Neurobiology Research
Research Tools
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Production of Targeted Mutations (Knockouts), feeder cells for ES cell lines
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ednrbtm1Nrd | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Noah Druckenbrod | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Ednrbflex3; | ||
| Mutation Made By | Noah Druckenbrod, University Of Wisconsin | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Expressed Gene | neo, neomycin resistance, bacterial | ||
| Molecular Note | A floxed neo cassette was placed upstream of exon 3 and an additional loxP site was inserted downstream of exon 3. [MGI Ref ID J:140320] | ||
| Gene Symbol and Name | Ednrb, endothelin receptor type B | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | ABCDS; AU022549; ET-B; ET-BR; ETB; ETBR; ETR-b; ETRB; Ednra; HSCR; HSCR2; Sox10m1; WS4A; expressed sequence AU022549; piebald; s; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ednrbtm1Nrd STD PCR, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Druckenbrod NR; Powers PA; Bartley CR; Walker JW; Epstein ML. 2008. Targeting of endothelin receptor-B to the neural crest. Genesis 46(8):396-400. [PubMed: 18693272] [MGI Ref ID J:140320]
Additional References
Ednrbtm1Nrd relatedAdameyko I; Lallemend F; Furlan A; Zinin N; Aranda S; Kitambi SS; Blanchart A; Favaro R; Nicolis S; Lubke M; Muller T; Birchmeier C; Suter U; Zaitoun I; Takahashi Y; Ernfors P. 2012. Sox2 and Mitf cross-regulatory interactions consolidate progenitor and melanocyte lineages in the cranial neural crest. Development 139(2):397-410. [PubMed: 22186729] [MGI Ref ID J:178973]
Druckenbrod NR; Epstein ML. 2009. Age-dependent changes in the gut environment restrict the invasion of the hindgut by enteric neural progenitors. Development 136(18):3195-203. [PubMed: 19700623] [MGI Ref ID J:152317]
Erickson CS; Zaitoun I; Haberman KM; Gosain A; Druckenbrod NR; Epstein ML. 2012. Sacral neural crest-derived cells enter the aganglionic colon of Ednrb-/- mice along extrinsic nerve fibers. J Comp Neurol 520(3):620-32. [PubMed: 21858821] [MGI Ref ID J:187343]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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