Strain Name:

B6;129-Postntm1Jmol/J

Stock Number:

009067

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
Homozyous Postn (periostin, osteoblast specific factor) targeted mutation mice are more prone to ventricular rupture in the first 10 days after a myocardial infarction, but surviving mice show less fibrosis and better ventricular performance. They also show less fibrosis and hypertrophy following long-term pressure overload. This strain may be useful in studies of cardiac remodeling and hypertrophy.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Jeffery D. Molkentin,   Cincinnati Children's Hospital

Description
Homozyous targeted mutation mice are more prone to ventricular rupture in the first 10 days after a myocardial infarction, but surviving mice show less fibrosis and better ventricular performance. They also show less fibrosis and hypertrophy following long-term pressure overload. The developing feet and hearts of 1-day-old mice show no expression of protein. No significant protein is found in the extracellular matrix of the heart after postnatal development, although the valves show persistent expression within fibroblasts. Homozygotes are slightly smaller than wild-type and heterozygous animals. This strain may be useful in studies of cardiac remodeling and hypertrophy.

Development
Exons 4-10 were replaced with a neomycin resistance cassette placed in the reverse transcriptional orientation. A 129-derived embryonic stem (ES) cell line was used to create the mutation. Chimeric mice were crossed to C57BL/6. This line was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Postn
024186   B6N(Cg)-Postntm1.1(KOMP)Vlcg/J
View Strains carrying other alleles of Postn     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Postntm1Jmol/Postntm1Jmol

        involves: 129/Sv * C57BL/6
  • cardiovascular system phenotype
  • altered response to myocardial infarction
    • following induced myocardial infarct, mice exhibit less of an increase in the number of fibroblasts in the left ventricle compared to in wild-type mice   (MGI Ref ID J:140301)
    • following induced myocardial infarct, mice exhibit an increase in death during the first 10 days and a 2-fold increase in the rate of ventricular wall rupture but reduced fibrosis and overall size of scaring compared to similarly treated wild-type mice   (MGI Ref ID J:140301)
    • however, mice maintain better cardiac function compared to wild-type mice over the 8 weeks following the initial scar formation   (MGI Ref ID J:140301)
  • small heart
  • homeostasis/metabolism phenotype
  • altered response to myocardial infarction
    • following induced myocardial infarct, mice exhibit less of an increase in the number of fibroblasts in the left ventricle compared to in wild-type mice   (MGI Ref ID J:140301)
    • following induced myocardial infarct, mice exhibit an increase in death during the first 10 days and a 2-fold increase in the rate of ventricular wall rupture but reduced fibrosis and overall size of scaring compared to similarly treated wild-type mice   (MGI Ref ID J:140301)
    • however, mice maintain better cardiac function compared to wild-type mice over the 8 weeks following the initial scar formation   (MGI Ref ID J:140301)
  • decreased susceptibility to injury
    • following transverse aortic constriction to induce pressure overload, mice exhibit less cardiac hypertrophy with reduced myocyte cross-section diameter and increased ventricular function after 8 weeks compared to similarly treated wild-type mice   (MGI Ref ID J:140301)
    • however, initial response during the first 2 weeks after pressure overload is normal   (MGI Ref ID J:140301)
  • cellular phenotype
  • abnormal cell adhesion
    • isolated fibroblasts exhibit reduced adhesion to neonatal cardiomyocytes compared to wild-type fibroblasts   (MGI Ref ID J:140301)
  • growth/size/body phenotype
  • decreased body weight

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Postntm1Jmol/Postntm1Jmol

        involves: 129S/SvEv
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • cyclosporine A-treated mice exhibit normal hypertrophic cardiomyopathy   (MGI Ref ID J:165269)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Heart Abnormalities
      cardiomyopathy

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Postntm1Jmol
Allele Name targeted mutation 1, Jeffery D Molkentin
Allele Type Targeted (knock-out)
Common Name(s) Pn-;
Mutation Made By Jeffery Molkentin,   Cincinnati Children's Hospital
Strain of Origin129/Sv
ES Cell Line Strain129
Gene Symbol and Name Postn, periostin, osteoblast specific factor
Chromosome 3
Gene Common Name(s) A630052E07Rik; AI747096; OSF-2; OSF2; PDLPOSTN; PN; Periostin; Plf; RIKEN cDNA A630052E07 gene; expressed sequence AI747096; peri;
Molecular Note Exons 4 through 10 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on P1 developing foot and heart extracts. [MGI Ref ID J:140301]

Genotyping

Genotyping Information

Genotyping Protocols

Postntm1Jmol STD PCR, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Oka T; Xu J; Kaiser RA; Melendez J; Hambleton M; Sargent MA; Lorts A; Brunskill EW; Dorn GW 2nd; Conway SJ; Aronow BJ; Robbins J; Molkentin JD. 2007. Genetic manipulation of periostin expression reveals a role in cardiac hypertrophy and ventricular remodeling. Circ Res 101(3):313-21. [PubMed: 17569887]  [MGI Ref ID J:140301]

Additional References

Postntm1Jmol related

Bozyk PD; Bentley JK; Popova AP; Anyanwu AC; Linn MD; Goldsmith AM; Pryhuber GS; Moore BB; Hershenson MB. 2012. Neonatal periostin knockout mice are protected from hyperoxia-induced alveolar simplication. PLoS One 7(2):e31336. [PubMed: 22363622]  [MGI Ref ID J:185317]

Cheek JD; Wirrig EE; Alfieri CM; James JF; Yutzey KE. 2012. Differential activation of valvulogenic, chondrogenic, and osteogenic pathways in mouse models of myxomatous and calcific aortic valve disease. J Mol Cell Cardiol 52(3):689-700. [PubMed: 22248532]  [MGI Ref ID J:183712]

Jelen S; Gena P; Lebeck J; Rojek A; Praetorius J; Frokiaer J; Fenton RA; Nielsen S; Calamita G; Rutzler M. 2012. Aquaporin-9 and urea transporter-A gene deletions affect urea transmembrane passage in murine hepatocytes. Am J Physiol Gastrointest Liver Physiol 303(11):G1279-87. [PubMed: 23042941]  [MGI Ref ID J:193645]

Lorts A; Schwanekamp JA; Baudino TA; McNally EM; Molkentin JD. 2012. Deletion of periostin reduces muscular dystrophy and fibrosis in mice by modulating the transforming growth factor-beta pathway. Proc Natl Acad Sci U S A 109(27):10978-83. [PubMed: 22711826]  [MGI Ref ID J:186422]

Lorts A; Schwanekamp JA; Elrod JW; Sargent MA; Molkentin JD. 2009. Genetic manipulation of periostin expression in the heart does not affect myocyte content, cell cycle activity, or cardiac repair. Circ Res 104(1):e1-7. [PubMed: 19038863]  [MGI Ref ID J:156434]

Teekakirikul P; Eminaga S; Toka O; Alcalai R; Wang L; Wakimoto H; Nayor M; Konno T; Gorham JM; Wolf CM; Kim JB; Schmitt JP; Molkentin JD; Norris RA; Tager AM; Hoffman SR; Markwald RR; Seidman CE; Seidman JG. 2010. Cardiac fibrosis in mice with hypertrophic cardiomyopathy is mediated by non-myocyte proliferation and requires Tgf-beta. J Clin Invest 120(10):3520-9. [PubMed: 20811150]  [MGI Ref ID J:165269]

Tkatchenko TV; Moreno-Rodriguez RA; Conway SJ; Molkentin JD; Markwald RR; Tkatchenko AV. 2009. Lack of periostin leads to suppression of Notch1 signaling and calcific aortic valve disease. Physiol Genomics 39(3):160-8. [PubMed: 19723774]  [MGI Ref ID J:155773]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThe donating investigator reports that homozygous females do not breed.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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