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| The Id1GFP "knock-in" allele both abolishes endogenous inhibitor of DNA binding 1 (Id1) gene function and expresses enhanced green fluorescent protein (EGFP) from the Id1 promoter/enhancer elements. These Id1/GFP mutant mice allow fluorescent monitoring of Id1 expression in the bone marrow (granulocyte and macrophage progenitors as well as downstream myeloid lineage cells) and may be useful for studying long-term repopulating hematopoietic stem cell maintenance and myeloid-versus-lymphoid lineage decisions. | |||||||||||||||
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Description
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Xiao-Hong Sun, Oklahoma Medical Research Foundation Important Note
Because the Id1GFP mutation originated in 129X1-derived ES cells that harbor the agouti allele (of the nonagouti locus) nearby on chromosome 2, homozygous mutant mice will likely have agouti coat color.Description
Homozygous Id1/GFP mice (Id1GFP/GFP) are viable and fertile; harboring an enhanced green fluorescent protein (EGFP) "knock-in" allele that both abolishes endogenous Id1 gene function and expresses EGFP from the Id1 promoter/enhancer elements. As such, EGFP fluorescence is directed to Mac1+/Ly6G+ myeloid lineage bone marrow cells (although rare fluorescence is reported in B220+ and/or CD19+ bone marrow cells). Homozygotes exhibit decreased long-term repopulating of hematopoietic stem cell (HSC) populations and a ~40% reduction in SLAM positive HSC. These Id1/GFP mutant mice allow fluorescent monitoring of Id1 expression in the bone marrow (granulocyte and macrophage progenitors as well as downstream myeloid lineage cells) and may be useful for studying HSC maintenance and myeloid-versus-lymphoid lineage decisions.NOTE:: Because the Id1GFP mutation originated in 129X1-derived ES cells that harbor the agouti allele (of the nonagouti locus) nearby on chromosome 2, homozygous mutant mice will likely have agouti coat color.
Development
A targeting construct was designed to insert an enhanced green fluorescent protein sequence (EGFP) sequence and a loxP-flanked pGK-Neo cassette into the 5' UTR of exon 1 of the targeted gene. This construct was inserted into the targeted gene via electroporation into 129X1/SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with EIIa-cre mice (on a C57BL/6 genetic background; see Stock No. 003724) to remove the neo selection cassette. Mice lacking both the floxed-pGK-neo and the EIIa-cre transgene were subsequently backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, these Id1/GFP mutant mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Id1tm1Xhsu/Id1tm1Xhsu
B6.129X1-Id1tm1Xhsu
- hematopoietic system phenotype
- decreased hematopoietic stem cell number (MGI Ref ID J:144696)
- there is a 45% reduction in the number of long-term HSC in whole bone marrow cells based on the ability to generate multilineage engraftment after serial transplantation in irradiated hosts
- similar results are observed when fractions of bone marrow cells enriched in stem cells are used in serial transplantation of irradiated hosts
- multilineage engraftment in the primary bone marrow transplant is the same when mutant or wild-type mice are the donors, indicating short term HSC cells are unaffected
This mouse can be used to support research in many areas including:
Developmental Biology Research
Lymphoid Tissue Defects
hematopoietic defects
Hematological Research
Hematopoietic Defects
Immunology and Inflammation Research
Lymphoid Tissue Defects
hematopoietic development
Research Tools
Developmental Biology Research
transplantation marker for embryonic and adult tissue
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: cell marker for bone marrow transplantation
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Hematological Research
Immunology and Inflammation Research
Macrophage Deficiency
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Id1tm1Xhsu | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Xiao-Hong Sun | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Id1GFP; | ||
| Mutation Made By | Xiao-Hong Sun, Oklahoma Medical Research Foundation | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Id1, inhibitor of DNA binding 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | AI323524; D2Wsu140e; DNA segment, Chr 2, Wayne State University 140, expressed; ID; ID125A; Idb1; MGC156482; bHLHb24; expressed sequence AI323524; | ||
| Molecular Note | An eGFP vector and a floxed neomycin cassette was knocked into the untranslated region of exon 1 of the gene locus. Founder mice were crossed with EIIa-cre transgenic mice to remove the neomycin cassette, leaving behind a loxP site. GFP expression was detected in bone marrow cells of myeloid lineages in both heterozygote and homozygote mice. Endogenous protein was not detected in immunobloting of cytokine-stimulated bone marrow cells from homozygote mice confirming this is a null allele. [MGI Ref ID J:144696] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Cochrane SW; Zhao Y; Welner RS; Sun XH. 2009. Balance between Id and E proteins regulates myeloid-versus-lymphoid lineage decisions. Blood 113(5):1016-26. [PubMed: 18927439] [MGI Ref ID J:144569]
Perry SS; Zhao Y; Nie L; Cochrane SW; Huang Z; Sun XH. 2007. Id1, but not Id3, directs long-term repopulating hematopoietic stem-cell maintenance. Blood 110(7):2351-60. [PubMed: 17622570] [MGI Ref ID J:144696]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Because the Id1GFP mutation originated in 129X1-derived ES cells that harbor the agouti allele (of the nonagouti locus) nearby on chromosome 2, homozygous mutant mice will likely have agouti coat color.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
|
| Important Note | |
| Because the Id1GFP mutation originated in 129X1-derived ES cells that harbor the agouti allele (of the nonagouti locus) nearby on chromosome 2, homozygous mutant mice will likely have agouti coat color. | |
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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