Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Anthony Wynshaw-Boris,   UCSF

Description
These mice possess loxP sites flanking exons 4-6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 6 deleted, the loxP site flanked hygro resistance cassette deleted, or both excised in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in a wide range of tissues (see Stock No. 003314 for example), this mutant mouse strain may be useful in studies of Miller-Dieker syndrome, embryonic development and craniofacial defects.

When bred to a strain with Cre recombinase expression in a wide range of tissues, especially mammary gland (see Stock No. 003553 for example), this mutant mouse strain may be useful in studies of mammary gland development and tumorigenesis.

Development
A loxP site flanked targeting vector containing hygromycin resistance and thymidine kinase genes under the control of the cytomegalovirus promoter was utilized in the construction of this mutant. This selection cassette was inserted into intron 3 of the targeted gene, and another loxP site was inserted into intron 6. This construct was electroporated into 129S6/SvEvTac derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to 129S6 mice. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ mice (Stock No. 002448) for at least one generation to establish the colony.

Control Information

	Control
	Wild-type from the colony
	002448 129S1/SvImJ
Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Mnt^tm1Awb/Mnt⁺

        involves: 129S6/SvEvTac

• no phenotypic analysis
• *normal* no phenotypic analysis
  • mice were only used to generate Mnt^tm1.1Awb mice   (MGI Ref ID J:90397)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Mnt^tm1Awb/Mnt^tm1.1Awb Tg(MMTV-cre)4Mam/?

        involves: 129S6/SvEvTac * FVB   (conditional)

• reproductive system phenotype
• abnormal mammary gland morphology
  • involution is delayed at I2, I5, and I16   (MGI Ref ID J:110115)
  • apoptosis levels peak at day 5 of involution compared to day 2 in wild-type mice   (MGI Ref ID J:110115)
  • apoptosis in the alveoli and ducts is delayed and fewer cells undergo apoptosis   (MGI Ref ID J:110115)
• • abnormal involution of the mammary gland
    • during involution after weaning, mice display enlarged ducts   (MGI Ref ID J:110115)
    • after involution ducts remain enlarged   (MGI Ref ID J:110115)
    • nonvirgin females exhibit 90% enlarged ducts compared to 40% in nonvirgin control mice and virgin mice exhibit 50% enlarged ducts compared to 30% in virgin control mice   (MGI Ref ID J:110115)
• tumorigenesis
• mammary adenocarcinoma
  • in 7 of 11 continuously mated females with tumor latency ranging from 6 to 24 months compared to control mice that do not develop tumors some tumors show lymphocyte invasion   (MGI Ref ID J:110115)
  • tumors are invasive carcinomas that include solid tubular carcinomas, tubular carcinomas and carcinomas with sarcomatous changes   (MGI Ref ID J:110115)
  • tumors display ductal enlargement that may or may not be pre-existing   (MGI Ref ID J:110115)
• endocrine/exocrine gland phenotype
• abnormal mammary gland morphology
  • involution is delayed at I2, I5, and I16   (MGI Ref ID J:110115)
  • apoptosis levels peak at day 5 of involution compared to day 2 in wild-type mice   (MGI Ref ID J:110115)
  • apoptosis in the alveoli and ducts is delayed and fewer cells undergo apoptosis   (MGI Ref ID J:110115)
• • abnormal involution of the mammary gland
    • during involution after weaning, mice display enlarged ducts   (MGI Ref ID J:110115)
    • after involution ducts remain enlarged   (MGI Ref ID J:110115)
    • nonvirgin females exhibit 90% enlarged ducts compared to 40% in nonvirgin control mice and virgin mice exhibit 50% enlarged ducts compared to 30% in virgin control mice   (MGI Ref ID J:110115)
• integument phenotype
• abnormal mammary gland morphology
  • involution is delayed at I2, I5, and I16   (MGI Ref ID J:110115)
  • apoptosis levels peak at day 5 of involution compared to day 2 in wild-type mice   (MGI Ref ID J:110115)
  • apoptosis in the alveoli and ducts is delayed and fewer cells undergo apoptosis   (MGI Ref ID J:110115)
• • abnormal involution of the mammary gland
    • during involution after weaning, mice display enlarged ducts   (MGI Ref ID J:110115)
    • after involution ducts remain enlarged   (MGI Ref ID J:110115)
    • nonvirgin females exhibit 90% enlarged ducts compared to 40% in nonvirgin control mice and virgin mice exhibit 50% enlarged ducts compared to 30% in virgin control mice   (MGI Ref ID J:110115)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Mnttm1Awb
Allele Name		targeted mutation 1, Anthony Wynshaw-Boris
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		MntCKO;
Mutation Made By		Anthony Wynshaw-Boris,   UCSF
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		TC1/TC-1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Mnt, max binding protein
Chromosome		11
Gene Common Name(s)		MAD6; MXD6; ROX; bHLHd3;
Molecular Note		A loxP-flanked hygro resistance gene and another loxP site were inserted into introns 3 and 6, respectively. Southern blot confirmed recombination. [MGI Ref ID J:90397]

Genotyping

Genotyping Information

Genotyping Protocols

Mnt^tm1Awb, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Toyo-oka K; Hirotsune S; Gambello MJ; Zhou ZQ; Olson L; Rosenfeld MG; Eisenman R; Hurlin P; Wynshaw-Boris A. 2004. Loss of the Max-interacting protein Mnt in mice results in decreased viability, defective embryonic growth and craniofacial defects: relevance to Miller-Dieker syndrome. Hum Mol Genet 13(10):1057-67. [PubMed: 15028671]  [MGI Ref ID J:90397]

Additional References

Mnt^tm1Awb related

Toyo-oka K; Bowen TJ; Hirotsune S; Li Z; Jain S; Ota S; Lozach LE; Bassett IG; Lozach J; Rosenfeld MG; Glass CK; Eisenman R; Ren B; Hurlin P; Wynshaw-Boris A. 2006. Mnt-deficient mammary glands exhibit impaired involution and tumors with characteristics of myc overexpression. Cancer Res 66(11):5565-73. [PubMed: 16740691]  [MGI Ref ID J:110115]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
	002448 129S1/SvImJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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