Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   26-OCT-10 Species laboratory mouse Generation N10+N3 F2 (27-OCT-10) Generation Definitions   Donating Investigator IMR Colony,   The Jackson Laboratory

Important Note
The Myd88-deficient allele encodes a deletion of exon 3 of the myeloid differentiation primary response gene 88 locus. Myd88-deficiency is associated with a number of immune system abnormalities, as well as hematopoietic system and apoptotic abnormalities.

Description
Homozygous mice are viable and fertile. The Myd88-deficient allele encodes a deletion of exon 3 of the myeloid differentiation primary response gene 88 locus. Myd88-deficiency is associated with a number of immune system abnormalities, as well as hematopoietic system, molecular signaling, and apoptotic abnormalities.

Development
Myd88^fl mice, harboring loxP sites on either side of exon 3 of the Myd88 gene, arrived at The Jackson Laboratory congenic on the C57BL/6J genetic background (see Stock No. 008888). Upon arrival, some of these Myd88^fl mice were bred with C57BL/6-Tg(Zp3-Cre)93Knw/J mice (Stock No. 003651) for germline removal of loxP-flanked exon 3. Offspring with the floxed exon 3 removed were identified and then selectively bred to C57BL/6J inbred mice to remove the Cre transgene. The resulting mice, heterozygous for the Myd88-deficient allele, were subsequently bred together, to wildtype siblings, or to C57BL/6J inbred mice to establish this strain (as Stock No. 009088).

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myd88

008888	B6.129P2(SJL)-Myd88tm1Defr/J
016133	C57BL/6-Tg(Defa2-Myd88)1Lvh/J
009108	CBy.129P2(B6)-Myd88tm1Defr/J
012622	NOD.Cg-Myd88tm1Defr/J

View Strains carrying other alleles of Myd88     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Myd88^tm1.1Defr/Myd88⁺

        involves: 129P2/OlaHsd * C57BL/6

• no phenotypic analysis
• *normal* no phenotypic analysis   (MGI Ref ID J:139001)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research
Endogenous Regulators

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Cell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction
Transcriptional Regulation

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules

Research Tools
Apoptosis Research
Cancer Research
Cell Biology Research
Immunology and Inflammation Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Myd88tm1.1Defr
Allele Name		targeted mutation 1.1, Anthony L DeFranco
Allele Type		Targeted (knock-out)
Mutation Made By		Anthony DeFranco,   UCSF
Strain of Origin		129P2/OlaHsd
Gene Symbol and Name		Myd88, myeloid differentiation primary response gene 88
Chromosome		9
Gene Common Name(s)		MYD88D;
Molecular Note		Mice carrying the Myd88tm1Defr allele were crossed with Tg(Zp3-cre)93Knw mice to remove exon 3. [MGI Ref ID J:139001]

Genotyping

Genotyping Information

Genotyping Protocols

Myd88^tm1.1Defr, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hou B; Reizis B; Defranco AL. 2008. Toll-like Receptors Activate Innate and Adaptive Immunity by using Dendritic Cell-Intrinsic and -Extrinsic Mechanisms. Immunity 29(2):272-82. [PubMed: 18656388]  [MGI Ref ID J:139001]

Additional References

Myd88^tm1.1Defr related

Barker TT; Lee PY; Kelly-Scumpia KM; Weinstein JS; Nacionales DC; Kumagai Y; Akira S; Croker BP; Sobel ES; Reeves WH; Satoh M. 2011. Pathogenic role of B cells in the development of diffuse alveolar hemorrhage induced by pristane. Lab Invest 91(10):1540-50. [PubMed: 21808234]  [MGI Ref ID J:176270]

Bates JT; Graff AH; Phipps JP; Grayson JM; Mizel SB. 2011. Enhanced Antigen Processing of Flagellin Fusion Proteins Promotes the Antigen-Specific CD8+ T Cell Response Independently of TLR5 and MyD88. J Immunol 186(11):6255-62. [PubMed: 21515787]  [MGI Ref ID J:173205]

Bosmann M; Patel VR; Russkamp NF; Pache F; Zetoune FS; Sarma JV; Ward PA. 2011. MyD88-dependent production of IL-17F is modulated by the anaphylatoxin C5a via the Akt signaling pathway. FASEB J 25(12):4222-32. [PubMed: 21859896]  [MGI Ref ID J:178398]

Fukuzawa N; Petro M; Baldwin WM 3rd; Gudkov AV; Fairchild RL. 2011. A TLR5 agonist inhibits acute renal ischemic failure. J Immunol 187(7):3831-9. [PubMed: 21890657]  [MGI Ref ID J:179325]

Mohamadzadeh M; Pfeiler EA; Brown JB; Zadeh M; Gramarossa M; Managlia E; Bere P; Sarraj B; Khan MW; Pakanati KC; Ansari MJ; O'Flaherty S; Barrett T; Klaenhammer TR. 2011. Regulation of induced colonic inflammation by Lactobacillus acidophilus deficient in lipoteichoic acid. Proc Natl Acad Sci U S A 108 Suppl 1:4623-30. [PubMed: 21282652]  [MGI Ref ID J:170355]

Park K; Scott AL. 2010. Cholesterol 25-hydroxylase production by dendritic cells and macrophages is regulated by type I interferons. J Leukoc Biol 88(6):1081-7. [PubMed: 20699362]  [MGI Ref ID J:166647]

Watts BA 3rd; George T; Sherwood ER; Good DW. 2011. Basolateral LPS inhibits NHE3 and HCOFormula absorption through TLR4/MyD88-dependent ERK activation in medullary thick ascending limb. Am J Physiol Cell Physiol 301(6):C1296-306. [PubMed: 21881005]  [MGI Ref ID J:178333]

Xue X; Feng T; Yao S; Wolf KJ; Liu CG; Liu X; Elson CO; Cong Y. 2011. Microbiota downregulates dendritic cell expression of miR-10a, which targets IL-12/IL-23p40. J Immunol 187(11):5879-86. [PubMed: 22068236]  [MGI Ref ID J:179678]

Yoshida H; Russell J; Senchenkova EY; Almeida Paula LD; Granger DN. 2010. Interleukin-1beta mediates the extra-intestinal thrombosis associated with experimental colitis. Am J Pathol 177(6):2774-81. [PubMed: 20971730]  [MGI Ref ID J:170780]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.
Mating System	Homozygote x Homozygote         (Female x Male)   26-OCT-10
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Important Note

The Myd88-deficient allele encodes a deletion of exon 3 of the myeloid differentiation primary response gene 88 locus. Myd88-deficiency is associated with a number of immune system abnormalities, as well as hematopoietic system and apoptotic abnormalities.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
- Strain(s) not available to companies or for-profit entities.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.