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| Mice harboring this mNF-H-cre transgene have nuclear localized-Cre recombinase expression directed primarily to cortex and hippocampus neurons coinciding the late stages of brain development by the mouse neurofilament-H gene promoter. These mice may be useful in generating conditional knockouts for neurobiological, neurodevelopmental, neuromuscular, amyotrophic lateral sclerosis (ALS or Lou Gehrig's disease), or aging studies involving the neurons of the brain and spinal cord. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Ashok Kulkarni, NIDCR NIH Description
Mice hemizygous for this mNF-H-cre transgene are viable and fertile, with nuclear localized-Cre recombinase expression directed to neurons, coinciding with late stages of brain development, by the mouse neurofilament-H gene (Nefh) promoter. The cre expression in neurons (but not astrocytes) of the brain and spinal cord is mosaic with highest expression in the cortex and hippocampus. Cre recombinase activity begins around embryonic day (E)16.5-E18.5, with significantly robust activity observed after one week of age. The donating investigator reports that mNFHcre mouse line 12 homozygotes develop a neurological phenotype with seizures and become infertile and moribund. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in the resulting offspring.Development
The mNF-H-cre transgene was designed with the 8.5 kb promoter fragment from the mouse neurofilament-H gene (Nefh [or NF-H]; containing the 5' UTR and 40 bp of noncoding sequence of exon 1, ending just before the start codon) upstream of a Cre recombinase gene (with SV40 nuclear localization signal and polyA signal). This transgene was injected into the pronuclei of fertilized FVB/N oocytes. The resulting transgenic offspring were bred to FVB/N (NCI Frederick, MD) mice to establish founder line 12 (also called Tg mNF-H-cre 12 line mice or mNFHcre mouse line 12). Transgenic mice were then bred to floxed-Cdk5 mice (on a mixed C57BL/6;129/SvJ genetic background; see Stock No. 003536). The resulting offspring were bred together, and some of the mice harboring the mNFHcre line 12 transgene were bred together to select away from the Cdk5 mutation. Next, mNFHcre mouse line 12 (on a mixed C57BL/6;129/SvJ;FVB/N genetic background and no longer harboring the floxed-Cdk5) were bred to wildtype siblings for approximately 15 generations prior to arrival at The Jackson Laboratory. Upon arrival, mNFHcre mouse line 12 were bred with FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (311 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Epilepsy
Reproductive Biology Research
Fertility Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Nefh-cre)12Kul | ||
|---|---|---|---|
| Allele Name | transgene insertion 12, Ashok B Kulkarni | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg mNF-H-cre 12; | ||
| Mutation Made By | Ashok Kulkarni, NIDCR NIH | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Nefh, neurofilament, heavy polypeptide, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Nefh-cre)12Kul, transgene insertion 12, Ashok B Kulkarni | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg mNF-H-cre 12; | ||
| Driver Note | Nefh | ||
| Molecular Note | An 8.5 kb promoter fragment of mouse Nefh, containing the 5' UTR and 40 bp of non-coding sequence of exon 1 ending before the start codon, was adjoined to sequence encoding cre recombinase with an SV40 nuclear localization signal. An SV40 polyadenylationsignal was also included in the construct. Expression of cre recombinase is driven in neurons of the brain and spinal cord during late development (around E18.5). Line 12 is best described and is representative of the other lines. [MGI Ref ID J:82025] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hirasawa M; Cho A; Sreenath T; Sauer B; Julien JP; Kulkarni AB. 2001. Neuron-specific expression of Cre recombinase during the late phase of brain development. Neurosci Res 40(2):125-32. [PubMed: 11377750] [MGI Ref ID J:82025]
Hirasawa M; Ohshima T; Takahashi S; Longenecker G; Honjo Y; Veeranna; Pant HC; Mikoshiba K; Brady RO; Kulkarni AB. 2004. Perinatal abrogation of Cdk5 expression in brain results in neuronal migration defects. Proc Natl Acad Sci U S A 101(16):6249-54. [PubMed: 15067135] [MGI Ref ID J:89561]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together, to noncarrier (wildtype) siblings, or to FVB/NJ inbred mice (Stock No. 001800). The donating investigator reports that homozygous mice develop a neurological phenotype with seizures and become infertile and moribund.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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