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| Mouse strains created by the Pleiades Promoter Project (Dr. Elizabeth M. Simpson, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Canada) are now available through the Mutant Mouse Regional Resource Center (MMRRC) at The Jackson Laboratory. For the Ple54-EGFP mice, please see MMRRC #032925 at the MMRRC website. | ||||||||||||||||||||||
| These mice have the Ple54-EGFP transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human DCX gene directs expression of EGFP. | ||||||||||||||||||||||
- Description
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N7pN1
Generation DefinitionsDonating Investigator Elizabeth Simpson, University of British Columbia Description
These Ple54-EGFP/NLS;mEMS1927 mice have the Ple54-EGFP/NLS transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human doublecortin (DCX) gene directing expression of an enhanced green fluorescent protein (EGFP). These Ple54-EGFP/NLS;mEMS1927 mice may be useful in studying DCX-expressing cells in the brain and diseases affecting the brain.For Ple54-EGFP/NLS;mEMS1927 expression information, see The Pleiades Promoter Project website (Ple54 Promoter (pEMS1200)).
The EGFP expression pattern is described by the donating investigator as:
Detection of EGFP is strongest in soma of cells in the neurogenic regions of the adult brain: the subgranular zone of the dentate gyrus as well as the forebrain subventricular zone (rostral migratory stream and olfactory bulb). Additionally, EGFP-positive cell bodies are detected in the neuropil of the cortex, hippocampus and brainstem. Double label immunocytochemistry shows that the EGFP-positive cells of the forebrain subventricular zone and the rostral migratory stream co-express Doublecortin.These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia).
Development
The Ple54-EGFP/NLS transgene (pEMS1200) was designed with the 3687 bp Ple54 minipromoter (DCX-C; derived from a portion of the promoter and two downstream putative regulatory elements from the human doublecortin (DCX) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome via electroporation into mEMS1218 embryonic stem cells (generated from F1 generation male pups [hemizygous for the Hprtb-m3 allele and heterozygous for the Gt(ROSA)26Sortm1Sor allele] born from C57BL/6-congenic females homozygous for a Hprtb-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] and 129S-Gt(ROSA)26Sortm1Sor heterozygous males [see Stock No. 002171 and 003310, respectively]). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS1927) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino; Stock No. 000058) to establish the mutant colony. The donating investigator reports that these Ple54-EGFP/NLS;mEMS1927 mutant mice were bred with C57BL/6J wildtype mice for seven generations (and selectively removed the Gt(ROSA)26Sortm1Sor mutation) prior to sending to The Jackson Laboratory. Upon arrival, Ple54-EGFP/NLS;mEMS1927 mutant mice were used to cryopreserve sperm. When rederiving a live colony, sperm will be used along with oocytes from C57BL/6J inbred mice (Stock No. 000664) to generate obligate heterozygous females, and these females will be bred at least one generation to C57BL/6J inbred mice to establish the colony. The rederived mice may still be segregating for the Tyrc-2J mutation.For more information, see The Pleiades Promoter Project website (Ple54 Promoter (pEMS1200)).
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Hprttm13(Ple54-EGFP)Ems/Y
B6.129P2(Cg)-Hprttm13(Ple54-EGFP)Ems/Mmjax
- normal phenotype
- no abnormal phenotype detected
- heterozygous females and hemizygous males are viable and fertile (MGI Ref ID J:145689)
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: glial cells
Tissue/Cell Markers: glial cells, oligodendrocytes, Schwann cells
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Hprttm13(Ple54-EGFP)Ems | ||
|---|---|---|---|
| Allele Name | targeted mutation 13, Elizabeth M Simpson | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | DCX-EGFP; Hprt1tm13(Ple54-EGFP;mEMS1927)Ems; Hprt1tm13(mEMS1927)Ems; Ple54; | ||
| Mutation Made By | Elizabeth Simpson, University of British Columbia | ||
| Strain of Origin | (B6.129P2-Hprt | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Associated Marker Note | Expressed-count: 1Ex1-Source: JellyfishEx1-Gene: EGFPDriver-count: 1Dr1-Source: HumanDr1-Gene: 1641 | ||
| General Note | Germ line transmission of mutant cell line mEMS1927 has been established. | ||
| Molecular Note |
The Ple54-EGFP/NLS transgene (pEMS1200) was designed with the 3687 bp Ple54 minipromoter (DCX-C; derived from a portion of the promoter and two downstream putative regulatory elements from the human doublecortin (DCX) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. Detection of EGFP is strongest in soma of cells in the neurogenic regions of the adult brain: the subgranular zone of the dentate gyrusas well as the forebrain subventricular zone (rostral migratory stream and olfactory bulb). Additionally, EGFP-positive cell bodies are detected in the neuropil of the cortex, hippocampus and brainstem. Double label immunocytochemistry shows that the EGFP-positive cells of the forebrain subventricular zone and the rostral migratory stream co-express Doublecortin. [MGI Ref ID J:144244] [MGI Ref ID J:164356] | ||
| Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; Hprt1; expressed sequence C81579; hypoxanthine guanine phosphoribosyl transferase 1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Hprttm13(Ple54-EGFP)Ems relatedPortales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF; Bonaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Simpson EM:. 2010. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 107(38):16589-94. [PubMed: 20807748] [MGI Ref ID J:164356]
The Pleiades Promoter Project. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data Submission :. [MGI Ref ID J:145689]
Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2009. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics 93(3):196-204. [PubMed: 18950699] [MGI Ref ID J:144244]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males (Stock No. 000664).
Purchasing information
Pricing, Supply Level & Notes, Controls
This strain is currently Transferred.
General Supply Notes
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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