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| These mice have the Ple90-EGFP transgene targeted as a single copy "knockin" into the upstream region of the Hprt1 locus on the X chromosome. The promoter/regulatory regions of the human GFAP gene direct expression of EGFP. | |||||||||||||||
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Description
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Inbred (Female x Male) 01-SEP-09 Species laboratory mouse Donating Investigator Elizabeth Simpson, University of British Columbia Description
These Ple90-EGFP;mEMS1899 mice have the Ple90-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human glial fibrillary acidic protein (GFAP) gene directing expression of enhanced green fluorescent protein (EGFP). These Ple90-EGFP;mEMS1899 mice may be useful in studying GFAP-expressing cells in the brain and diseases affecting the brain.For Ple90-EGFP;mEMS1899 expression information, see The Pleiades Promoter Project website (Ple90 Promoter (pEMS1377)).
The donating investigator reports:
The expression pattern of the Ple90 MiniPromoter is quite similar to the expression of Ple88 (GFAP-A; see Stock No. 008706); with broad expression in glial cellular profiles throughout the brain and spinal cord. Numerous astroglial profiles are seen in the cortical grey and white matter and in the cerebellum the full extent of Bergmann glial processes are highlighted with anti-EGFP immunocytochemistry. EGFP-immunoreactivity is most prominent in the cell bodies across all regions, with a subpopulation of cells showing a halo of process staining.These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia).
Development
The Ple90-EGFP transgene (pEMS1377) was designed with the 1433 bp Ple90 minipromoter (GFAP-C; derived from a putative upstream regulatory region and a portion of the promoter of the human glial fibrillary acidic protein (GFAP) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt1b-m3 mutant locus on the X chromosome via electroporation into mEMS1204 embryonic stem cells (generated from F1 generation male pups born of C57BL/6-congenic females homozygous for a Hprt1b-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sortm1Sor heterozygous males (see Stock No. 002171 and 003310, respectively)). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS1899) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino; Stock No. 000058) to establish the mutant colony. These Ple90-EGFP;mEMS1899 mutant mice were bred with C57BL/6J wildtype mice for five generations. Black mice without the Gt(ROSA)26Sortm1Sor or Tyrc-2J mutations were sent to The Jackson Laboratory. Upon arrival, Ple90-EGFP;mEMS1899 mutant mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the live colony.For more information, see The Pleiades Promoter Project website (Ple90 Promoter (pEMS1377)).
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 100492 B6129PF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: glial cells
Tissue/Cell Markers: glial cells, oligodendrocytes, Schwann cells
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information
Currently there are no related genes or alleles for this strain.Genotyping
Genotyping Information
Genotyping Protocols
Hprt1tm18(Ple90-EGFP)Ems, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males (Stock No. 000664). Mating System Heterozygote x Inbred (Female x Male) 01-SEP-09
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 100492 B6129PF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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