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| The Axin2lacZ mutation both abolishes endogenous gene function and expresses NLS-lacZ under the control of the endogenous promoter/enhancer regions. Homozygous mice exhibit a phenotype resembling craniosynostosis in humans. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x +/+ sibling (Female x Male) 23-MAR-10 Species laboratory mouse Generation N16+F7 (29-APR-13)
Generation DefinitionsDonating Investigator Walter Birchmeier, Max-Delbrueck-Center for Mol. Medicine Description
Homozygous mice are viable and fertile, with the Axin2lacZ (or conductinlacZ) mutation that both abolishes endogenous Axin2 gene function and expresses NLS-lacZ under the control of the endogenous Axin2 promoter/enhancer regions. Homozygous mice exhibit cranial skull defects and malformations of skull structures; a phenotype resembling craniosynostosis in humans. Specifically, homozygous mice show an obvious reduction in head growth within the first 3 weeks after birth, resulting from developmental defects of the cranial skull (premature fusion of cranial sutures) at early postnatal stages. Axin2-deficient mice have abnormal calvarial morphogenesis/osteoblast development. Because Axin2 is a negative regulator of the canonical Wnt pathway that suppress signal transduction by promoting β-catenin degradation, the NLS-lacZ expression in these Axin2lacZ (or conductinlacZ) mutant mice may be useful in monitoring endogenous canonical Wnt signals in many tissues and organs during development, regeneration and tumorigenesis.Development
A targeting vector was used delete most of exon 2 of the Axin2 (Axin2/conductin/Axil) locus via in-frame insertion of a nuclear-localized β-galactosidase (NLS-lacZ) gene (followed by a polyA signal and neo cassette) into the endogenous Axin2 start codon. This construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. ES cells were injected into recipient blastocysts, and chimeric mice were bred with C57BL/6NCrl mice to establish the mutant colony. The donating investigator reports that mutant mice were subsequently backcrossed to C57BL/6NCrl inbred mice for 15 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony. During the backcross, the Y chromosome may not have been fixed to the C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
018867 B6.129(Cg)-Axin2tm1(cre/ERT2)Rnu/J 016997 B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Craniosynostosis, Type 1; CRS1
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Colorectal Cancer; CRC (AXIN2)
Oligodontia-Colorectal Cancer Syndrome; ODCRCS (AXIN2)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Axin2tm1Wbm/Axin2tm1Wbm
involves: 129 * C57BL/6
- craniofacial phenotype
- abnormal cranium morphology
- adult skulls became abnormally structured and shaped due to premature suture closure (MGI Ref ID J:98523)
- abnormal cranial suture morphology
- the Jugum Limitans, which separates the frontal and nasal bones, were often missing (MGI Ref ID J:98523)
- abnormal neurocranium morphology
- enhanced osteoblast differentiation predominantly in the anterior calvarium (MGI Ref ID J:98523)
- decreased cranium height (MGI Ref ID J:98523)
- shortened head
- reduced growth of the head, obvious within 3 weeks after birth (MGI Ref ID J:98523)
- skeleton phenotype
- abnormal cranium morphology
- adult skulls became abnormally structured and shaped due to premature suture closure (MGI Ref ID J:98523)
- abnormal cranial suture morphology
- the Jugum Limitans, which separates the frontal and nasal bones, were often missing (MGI Ref ID J:98523)
- abnormal neurocranium morphology
- enhanced osteoblast differentiation predominantly in the anterior calvarium (MGI Ref ID J:98523)
- decreased cranium height (MGI Ref ID J:98523)
- abnormal osteoblast physiology
- enhanced proliferation of osteoblast precursors in the developing metopic suture and of isolated primary osteoblasts from nasal/frontal bones (cranial neural crest derived) but not from parietal bones (mesoderm-derived) (MGI Ref ID J:98523)
- enhanced osteoblast differentiation predominantly in the anterior calvarium (MGI Ref ID J:98523)
- primary calvarial osteoblasts isolated from nasal/frontal bones exhibited enhanced formation of mineralized nodules when induced to differentiate (MGI Ref ID J:98523)
- abnormal skeleton development
- accelerated skeletogenesis (MGI Ref ID J:98523)
- premature intramembranous bone ossification
- in the skull (MGI Ref ID J:98523)
- premature suture closure
- premature closure of the metopic suture, with the suture remaining patent at birth but fused by P8 (MGI Ref ID J:98523)
- vision/eye phenotype
- abnormal eye morphology
- requently exhibited eye abnormalities (MGI Ref ID J:98523)
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Developmental Biology Research
Craniofacial and Palate Defects
Growth Defects
Growth Defects (homozygous)
Internal/Organ Defects
brain
Neurobiology Research
lacZ expression in neural tissue
Research Tools
lacZ Expression
Genetics Research
Tissue/Cell Markers
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Axin2tm1Wbm | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Walter Birchmeier | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Ax2-; Axin2-; Axin2NLSlacZ; Axin2lacZ; Axin2lacZki; Axin2tm1Jbeh; Conductinlz; conductinlacZ; | ||
| Mutation Made By | Walter Birchmeier, Max-Delbrueck-Center for Mol. Medicine | ||
| Strain of Origin | 129P2/OlaHsd | ||
| Site of Expression | LacZ with a nuclear localization signal (NLS-lacZ) replaces expression of the targeted gene and may be useful in monitoring endogenous canonical Wnt signals in many tissues during development, regeneration and tumorigenesis. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | A lacZ gene containing a nuclear localization signal was introduced in frame to the endogenous start codon by homologous recombination, thereby replacing most of exon 2. Beta-galactosidase staining reflected endogenous gene expression in the developingembryo. [MGI Ref ID J:74286] | ||
| Gene Symbol and Name | Axin2, axin2 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | AXIL; Conductin; ODCRCS; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Axin2tm1Wbm, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Lustig B; Jerchow B; Sachs M; Weiler S; Pietsch T; Karsten U; van de Wetering M; Clevers H; Schlag PM; Birchmeier W; Behrens J. 2002. Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors. Mol Cell Biol 22(4):1184-93. [PubMed: 11809809] [MGI Ref ID J:74286]
Additional References
Axin2tm1Wbm relatedAl Alam D; Green M; Tabatabai Irani R; Parsa S; Danopoulos S; Sala FG; Branch J; El Agha E; Tiozzo C; Voswinckel R; Jesudason EC; Warburton D; Bellusci S. 2011. Contrasting expression of canonical Wnt signaling reporters TOPGAL, BATGAL and Axin2(LacZ) during murine lung development and repair. PLoS One 6(8):e23139. [PubMed: 21858009] [MGI Ref ID J:176498]
Baker R; Kent CV; Silbermann RA; Hassell JA; Young LJ; Howe LR. 2010. Pea3 transcription factors and wnt1-induced mouse mammary neoplasia. PLoS One 5(1):e8854. [PubMed: 20107508] [MGI Ref ID J:157624]
Behr B; Longaker MT; Quarto N. 2010. Differential activation of canonical Wnt signaling determines cranial sutures fate: a novel mechanism for sagittal suture craniosynostosis. Dev Biol 344(2):922-40. [PubMed: 20547147] [MGI Ref ID J:163650]
Blackburn J; Ohazama A; Kawasaki K; Otsuka-Tanaka Y; Liu B; Honda K; Rountree RB; Hu Y; Kawasaki M; Birchmeier W; Schmidt-Ullrich R; Kinoshita A; Schutte BC; Hammond NL; Dixon MJ; Sharpe PT. 2012. The role of Irf6 in tooth epithelial invagination. Dev Biol 365(1):61-70. [PubMed: 22366192] [MGI Ref ID J:184926]
Chai R; Xia A; Wang T; Jan TA; Hayashi T; Bermingham-McDonogh O; Cheng AG. 2011. Dynamic expression of lgr5, a wnt target gene, in the developing and mature mouse cochlea. J Assoc Res Otolaryngol 12(4):455-69. [PubMed: 21472479] [MGI Ref ID J:173579]
Chassot AA; Bradford ST; Auguste A; Gregoire EP; Pailhoux E; de Rooij DG; Schedl A; Chaboissier MC. 2012. WNT4 and RSPO1 together are required for cell proliferation in the early mouse gonad. Development 139(23):4461-72. [PubMed: 23095882] [MGI Ref ID J:189064]
Chassot AA; Gregoire EP; Lavery R; Taketo MM; de Rooij DG; Adams IR; Chaboissier MC. 2011. RSPO1/beta-catenin signaling pathway regulates oogonia differentiation and entry into meiosis in the mouse fetal ovary. PLoS One 6(10):e25641. [PubMed: 21991325] [MGI Ref ID J:179634]
Chassot AA; Ranc F; Gregoire EP; Roepers-Gajadien HL; Taketo MM; Camerino G; de Rooij DG; Schedl A; Chaboissier MC. 2008. Activation of beta-catenin signaling by Rspo1 controls differentiation of the mammalian ovary. Hum Mol Genet 17(9):1264-77. [PubMed: 18250098] [MGI Ref ID J:133933]
Dao DY; Yang X; Flick LM; Chen D; Hilton MJ; O'Keefe RJ. 2010. Axin2 regulates chondrocyte maturation and axial skeletal development. J Orthop Res 28(1):89-95. [PubMed: 19623616] [MGI Ref ID J:162154]
Fancy SP; Harrington EP; Yuen TJ; Silbereis JC; Zhao C; Baranzini SE; Bruce CC; Otero JJ; Huang EJ; Nusse R; Franklin RJ; Rowitch DH. 2011. Axin2 as regulatory and therapeutic target in newborn brain injury and remyelination. Nat Neurosci 14(8):1009-16. [PubMed: 21706018] [MGI Ref ID J:175912]
Fevr T; Robine S; Louvard D; Huelsken J. 2007. Wnt/beta-catenin is essential for intestinal homeostasis and maintenance of intestinal stem cells. Mol Cell Biol 27(21):7551-9. [PubMed: 17785439] [MGI Ref ID J:129073]
Haara O; Fujimori S; Schmidt-Ullrich R; Hartmann C; Thesleff I; Mikkola ML. 2011. Ectodysplasin and Wnt pathways are required for salivary gland branching morphogenesis. Development 138(13):2681-91. [PubMed: 21652647] [MGI Ref ID J:173573]
Hadjihannas MV; Bruckner M; Behrens J. 2010. Conductin/axin2 and Wnt signalling regulates centrosome cohesion. EMBO Rep 11(4):317-24. [PubMed: 20300119] [MGI Ref ID J:164744]
Hadjihannas MV; Bruckner M; Jerchow B; Birchmeier W; Dietmaier W; Behrens J. 2006. Aberrant Wnt/beta-catenin signaling can induce chromosomal instability in colon cancer. Proc Natl Acad Sci U S A 103(28):10747-52. [PubMed: 16815967] [MGI Ref ID J:111812]
Harris-Johnson KS; Domyan ET; Vezina CM; Sun X. 2009. beta-Catenin promotes respiratory progenitor identity in mouse foregut. Proc Natl Acad Sci U S A 106(38):16287-92. [PubMed: 19805295] [MGI Ref ID J:153236]
Hiremath M; Lydon JP; Cowin P. 2007. The pattern of beta-catenin responsiveness within the mammary gland is regulated by progesterone receptor. Development 134(20):3703-12. [PubMed: 17881490] [MGI Ref ID J:128375]
Huch M; Dorrell C; Boj SF; van Es JH; Li VS; van de Wetering M; Sato T; Hamer K; Sasaki N; Finegold MJ; Haft A; Vries RG; Grompe M; Clevers H. 2013. In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature 494(7436):247-50. [PubMed: 23354049] [MGI Ref ID J:194551]
Jan TA; Chai R; Sayyid ZN; van Amerongen R; Xia A; Wang T; Sinkkonen ST; Zeng YA; Levin JR; Heller S; Nusse R; Cheng AG. 2013. Tympanic border cells are Wnt-responsive and can act as progenitors for postnatal mouse cochlear cells. Development 140(6):1196-206. [PubMed: 23444352] [MGI Ref ID J:194845]
Khalil S; Tan GA; Giri DD; Zhou XK; Howe LR. 2012. Activation status of Wnt/ss-catenin signaling in normal and neoplastic breast tissues: relationship to HER2/neu expression in human and mouse. PLoS One 7(3):e33421. [PubMed: 22457761] [MGI Ref ID J:187135]
Kim BM; Mao J; Taketo MM; Shivdasani RA. 2007. Phases of canonical Wnt signaling during the development of mouse intestinal epithelium. Gastroenterology 133(2):529-38. [PubMed: 17681174] [MGI Ref ID J:128278]
Kim BM; Miletich I; Mao J; McMahon AP; Sharpe PA; Shivdasani RA. 2007. Independent functions and mechanisms for homeobox gene Barx1 in patterning mouse stomach and spleen. Development 134(20):3603-13. [PubMed: 17855428] [MGI Ref ID J:128378]
Kim S; Goel S; Alexander CM. 2011. Differentiation generates paracrine cell pairs that maintain basaloid mouse mammary tumors: proof of concept. PLoS One 6(4):e19310. [PubMed: 21541292] [MGI Ref ID J:172378]
Klaus A; Muller M; Schulz H; Saga Y; Martin JF; Birchmeier W. 2012. Wnt/beta-catenin and Bmp signals control distinct sets of transcription factors in cardiac progenitor cells. Proc Natl Acad Sci U S A 109(27):10921-6. [PubMed: 22711842] [MGI Ref ID J:186421]
Lin SL; Li B; Rao S; Yeo EJ; Hudson TE; Nowlin BT; Pei H; Chen L; Zheng JJ; Carroll TJ; Pollard JW; McMahon AP; Lang RA; Duffield JS. 2010. Macrophage Wnt7b is critical for kidney repair and regeneration. Proc Natl Acad Sci U S A 107(9):4194-9. [PubMed: 20160075] [MGI Ref ID J:158579]
Liu B; Yu HM; Hsu W. 2007. Craniosynostosis caused by Axin2 deficiency is mediated through distinct functions of beta-catenin in proliferation and differentiation. Dev Biol 301(1):298-308. [PubMed: 17113065] [MGI Ref ID J:117081]
Luis TC; Naber BA; Roozen PP; Brugman MH; de Haas EF; Ghazvini M; Fibbe WE; van Dongen JJ; Fodde R; Staal FJ. 2011. Canonical wnt signaling regulates hematopoiesis in a dosage-dependent fashion. Cell Stem Cell 9(4):345-56. [PubMed: 21982234] [MGI Ref ID J:177653]
Macias H; Moran A; Samara Y; Moreno M; Compton JE; Harburg G; Strickland P; Hinck L. 2011. SLIT/ROBO1 signaling suppresses mammary branching morphogenesis by limiting basal cell number. Dev Cell 20(6):827-40. [PubMed: 21664580] [MGI Ref ID J:180908]
Malanchi I; Peinado H; Kassen D; Hussenet T; Metzger D; Chambon P; Huber M; Hohl D; Cano A; Birchmeier W; Huelsken J. 2008. Cutaneous cancer stem cell maintenance is dependent on beta-catenin signalling. Nature 452(7187):650-3. [PubMed: 18385740] [MGI Ref ID J:133779]
Manuylov NL; Smagulova FO; Leach L; Tevosian SG. 2008. Ovarian development in mice requires the GATA4-FOG2 transcription complex. Development 135(22):3731-43. [PubMed: 18927154] [MGI Ref ID J:143586]
Maruyama T; Mirando AJ; Deng CX; Hsu W. 2010. The balance of WNT and FGF signaling influences mesenchymal stem cell fate during skeletal development. Sci Signal 3(123):ra40. [PubMed: 20501936] [MGI Ref ID J:185410]
Mikels A; Minami Y; Nusse R. 2009. Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling. J Biol Chem 284(44):30167-76. [PubMed: 19720827] [MGI Ref ID J:156970]
Minear S; Leucht P; Jiang J; Liu B; Zeng A; Fuerer C; Nusse R; Helms JA. 2010. Wnt proteins promote bone regeneration. Sci Transl Med 2(29):29ra30. [PubMed: 20427820] [MGI Ref ID J:167878]
Minear S; Leucht P; Miller S; Helms JA. 2010. rBMP represses Wnt signaling and influences skeletal progenitor cell fate specification during bone repair. J Bone Miner Res 25(6):1196-207. [PubMed: 20200943] [MGI Ref ID J:183367]
Mirando AJ; Maruyama T; Fu J; Yu HM; Hsu W. 2010. beta-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis. BMC Dev Biol 10:116. [PubMed: 21108844] [MGI Ref ID J:166753]
Mugford JW; Yu J; Kobayashi A; McMahon AP. 2009. High-resolution gene expression analysis of the developing mouse kidney defines novel cellular compartments within the nephron progenitor population. Dev Biol 333(2):312-23. [PubMed: 19591821] [MGI Ref ID J:152419]
Park JI; Venteicher AS; Hong JY; Choi J; Jun S; Shkreli M; Chang W; Meng Z; Cheung P; Ji H; McLaughlin M; Veenstra TD; Nusse R; McCrea PD; Artandi SE. 2009. Telomerase modulates Wnt signalling by association with target gene chromatin. Nature 460(7251):66-72. [PubMed: 19571879] [MGI Ref ID J:150343]
Qian L; Mahaffey JP; Alcorn HL; Anderson KV. 2011. Tissue-specific roles of Axin2 in the inhibition and activation of Wnt signaling in the mouse embryo. Proc Natl Acad Sci U S A 108(21):8692-7. [PubMed: 21555575] [MGI Ref ID J:171894]
Ren S; Johnson BG; Kida Y; Ip C; Davidson KC; Lin SL; Kobayashi A; Lang RA; Hadjantonakis AK; Moon RT; Duffield JS. 2013. LRP-6 is a coreceptor for multiple fibrogenic signaling pathways in pericytes and myofibroblasts that are inhibited by DKK-1. Proc Natl Acad Sci U S A 110(4):1440-5. [PubMed: 23302695] [MGI Ref ID J:193705]
Rooker SM; Liu B; Helms JA. 2009. Role of Wnt signaling in the biology of the periodontium. Dev Dyn 239(1):140-147. [PubMed: 19530172] [MGI Ref ID J:155261]
Sato T; van Es JH; Snippert HJ; Stange DE; Vries RG; van den Born M; Barker N; Shroyer NF; van de Wetering M; Clevers H. 2011. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature 469(7330):415-8. [PubMed: 21113151] [MGI Ref ID J:168728]
Soshnikova N; Zechner D; Huelsken J; Mishina Y; Behringer RR; Taketo MM; Crenshaw EB 3rd; Birchmeier W. 2003. Genetic interaction between Wnt/beta-catenin and BMP receptor signaling during formation of the AER and the dorsal-ventral axis in the limb. Genes Dev 17(16):1963-8. [PubMed: 12923052] [MGI Ref ID J:84878]
Suomalainen M; Thesleff I. 2009. Patterns of Wnt pathway activity in the mouse incisor indicate absence of Wnt/beta-catenin signaling in the epithelial stem cells. Dev Dyn 239(1):364-372. [PubMed: 19806668] [MGI Ref ID J:155223]
Talamillo A; Delgado I; Nakamura T; de-Vega S; Yoshitomi Y; Unda F; Birchmeier W; Yamada Y; Ros MA. 2010. Role of Epiprofin, a zinc-finger transcription factor, in limb development. Dev Biol 337(2):363-74. [PubMed: 19913006] [MGI Ref ID J:157263]
Ten Berge D; Brugmann SA; Helms JA; Nusse R. 2008. Wnt and FGF signals interact to coordinate growth with cell fate specification during limb development. Development 135(19):3247-57. [PubMed: 18776145] [MGI Ref ID J:138770]
Witte F; Chan D; Economides AN; Mundlos S; Stricker S. 2010. Receptor tyrosine kinase-like orphan receptor 2 (ROR2) and Indian hedgehog regulate digit outgrowth mediated by the phalanx-forming region. Proc Natl Acad Sci U S A 107(32):14211-6. [PubMed: 20660756] [MGI Ref ID J:163602]
Yan Y; Tang D; Chen M; Huang J; Xie R; Jonason JH; Tan X; Hou W; Reynolds D; Hsu W; Harris SE; Puzas JE; Awad H; O'Keefe RJ; Boyce BF; Chen D. 2009. Axin2 controls bone remodeling through the beta-catenin-BMP signaling pathway in adult mice. J Cell Sci 122(Pt 19):3566-78. [PubMed: 19737815] [MGI Ref ID J:153051]
Yu HM; Jerchow B; Sheu TJ; Liu B; Costantini F; Puzas JE; Birchmeier W; Hsu W. 2005. The role of Axin2 in calvarial morphogenesis and craniosynostosis. Development 132(8):1995-2005. [PubMed: 15790973] [MGI Ref ID J:98523]
Yu HM; Liu B; Costantini F; Hsu W. 2007. Impaired neural development caused by inducible expression of Axin in transgenic mice. Mech Dev 124(2):146-56. [PubMed: 17123792] [MGI Ref ID J:119927]
Zechner D; Muller T; Wende H; Walther I; Taketo MM; Crenshaw EB 3rd; Treier M; Birchmeier W; Birchmeier C. 2007. Bmp and Wnt/beta-catenin signals control expression of the transcription factor Olig3 and the specification of spinal cord neurons. Dev Biol 303(1):181-90. [PubMed: 17150208] [MGI Ref ID J:118813]
Zeng YA; Nusse R. 2010. Wnt proteins are self-renewal factors for mammary stem cells and promote their long-term expansion in culture. Cell Stem Cell 6(6):568-77. [PubMed: 20569694] [MGI Ref ID J:161359]
van Amerongen R; Bowman AN; Nusse R. 2012. Developmental Stage and Time Dictate the Fate of Wnt/beta-Catenin-Responsive Stem Cells in the Mammary Gland. Cell Stem Cell 11(3):387-400. [PubMed: 22863533] [MGI Ref ID J:186834]
van Amerongen R; Fuerer C; Mizutani M; Nusse R. 2012. Wnt5a can both activate and repress Wnt/beta-catenin signaling during mouse embryonic development. Dev Biol 369(1):101-14. [PubMed: 22771246] [MGI Ref ID J:187621]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6NJ inbred mice (Stock No. 005304). Mating System Heterozygote x +/+ sibling (Female x Male) 23-MAR-10 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Axin2tm1Wbm
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Axin2tm1Wbm x Wild-type for Axin2tm1Wbm $296.00 Wild-type for Axin2tm1Wbm x Heterozygous for Axin2tm1Wbm Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Axin2tm1Wbm
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Axin2tm1Wbm x Wild-type for Axin2tm1Wbm $384.80 Wild-type for Axin2tm1Wbm x Heterozygous for Axin2tm1Wbm Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.