Strain Name:

B6.129P2-P2ry2tm1Bhk/J

Stock Number:

009132

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These purinergic receptor P2Y, G-protein coupled 2 (P2ry2tm1Bhk) knock-in mice are susceptible to lung infections of Pseudomonas aeruginosa, exhibit impaired neutrophil chemotaxis, show a loss of sensitivity to thermal nociception and capsaicin, and have increased urinary concentration. This mutant mouse strain may be useful in studies of purinergic signaling in the regulation of ion transport, immune response to bacterial infections, pain response, neuronal differentiation and neurogenesis.

Description

Strain Information

Former Names B6.129-P2ry2tm1Bhk/J    (Changed: 24-FEB-09 )
Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   29-DEC-09
Specieslaboratory mouse
GenerationN13+F9 (11-DEC-13)
Generation Definitions
 
Donating Investigator Beverly H Koller,   Univ. of North Carolina at Chapel Hill

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of kidney tissue. Intracellular calcium ion levels in lung fibroblasts isolated from homozygotes fail to respond to nucleotide (UTP, ATP, ADP, UDP) challenge. Isolated airway epithelial cells have a loss of or diminished intracellular calcium response to nucleotide challenge. Unlike wild-type macrophages, macrophages derived from these animals fail to respond to extracellular stimulation with UTP. ATP- and adenosine 50[g-thio] triphosphate (ATPgS)-evoked aortic endothelium-dependent relaxation is reduced. Luminal nucleotide stimulated distal colonic ion transport is reduced. Homozygotes are more susceptible to lung infections of Pseudomonas aeruginosa, exhibit impaired neutrophil chemotaxis (loss in gradient sensing and migration distance), and loss of sensitivity to thermal nociception and capsaicin. Mutant mice fail to develop thermal hypersensitivity after to inflammatory injury (inflammatory hyperalgesia). Dorsal root ganglia exhibit diminished UTP-evoked Ca++ response. Growth-associated protein-43 (GAP-43) levels are not increased in sciatic nerves of mutants in response to ATPgammaS injection. Kidney function defects include increased renal collecting duct epithelial sodium channel activity and increased urine concentration. This mutant mouse strain may be useful in studies of purinergic signaling in the regulation of ion transport, immune response to bacterial infections, pain response, neuronal differentiation and neurogenesis.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt base pairs 552-1149 of the published cDNA sequence. The construct was electroporated into 129P2/OlaHsd derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reported that the resulting chimeric animals were crossed to C57BL/6NTac mice, and then backcrossed to the same for 12 generations (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

P2ry2tm1Bhk/P2ry2tm1Bhk

        involves: 129P2/OlaHsd * C57BL/6 * DBA/2
  • homeostasis/metabolism phenotype
  • abnormal ion homeostasis
    • intracellular calcium responses to nucleotides (UTP, ATP, ADP, UDP) are abolished in cultured lung fibroblasts   (MGI Ref ID J:57617)
    • intracellular calcium responses to UTP are abolished in tracheal and nasal epithelial cells and responses to ATP are substantially reduced in tracheal (74.5%) and nasal (44%) epithelial cells, however no reduction in calcium responses to ADP, 2-MeSATP, 2-MeSADP or UDP   (MGI Ref ID J:57617)
    • chloride secretory responses to UTP and ATP are reduced by 83-95% in excised tracheas and responses to alpha,beta-meATP (91%), 2-MeSATP (84%), and 2-MeSADP (54%) are substantially reduced, however no reduction in chloride secretory responses to UDP   (MGI Ref ID J:57618)
    • chloride secretory responses to ATP are reduced by 88% in excised gallbladders and responses to UTP are reduced by 30% in excised gallbladders, however no reduction in chloride secretory responses to UDP   (MGI Ref ID J:57618)
    • chloride secretory responses to nucleotides in the jejunum are not altered   (MGI Ref ID J:57618)
  • abnormal response/metabolism to endogenous compounds
    • intracellular calcium and inositol phosphate responses to nucleotides (UTP, ATP, ADP, UDP) are abolished in cultured lung fibroblasts   (MGI Ref ID J:57617)
    • intracellular calcium responses to UTP are abolished in tracheal and nasal epithelial cells and responses to ATP are substantially reduced in tracheal (74.5%) and nasal (44%) epithelial cells, however no reduction in calcium responses to ADP, 2-MeSATP, 2-MeSADP or UDP   (MGI Ref ID J:57617)
    • chloride secretory responses to UTP and ATP are reduced by 83-95% in excised tracheas and responses to alpha,beta-meATP (91%), 2-MeSATP (84%), and 2-MeSADP (54%) are substantially reduced, however no reduction in chloride secretory responses to UDP   (MGI Ref ID J:57618)
    • chloride secretory responses to ATP are reduced by 88% in excised gallbladders and responses to UTP are reduced by 30% in excised gallbladders, however no reduction in chloride secretory responses to UDP   (MGI Ref ID J:57618)
    • chloride secretory responses to nucleotides in the jejunum are not altered   (MGI Ref ID J:57618)

P2ry2tm1Bhk/P2ry2tm1Bhk

        involves: 129P2/OlaHsd
  • homeostasis/metabolism phenotype
  • abnormal response/metabolism to endogenous compounds
    • treatment with ATPgammaS in the presence of nerve growth factor does not increase dorsal root ganglion neuronal differentiation as in wild-type   (MGI Ref ID J:104694)
    • injection of ATPgammaS into the sciatic nerve does not increase growth-associated protein-43, a marker for axonal growth, as in wild-type   (MGI Ref ID J:104694)
  • nervous system phenotype
  • abnormal neuron differentiation
    • treatment with ATPgammaS in the presence of nerve growth factor does not increase dorsal root ganglion neuronal differentiation as in wild-type   (MGI Ref ID J:104694)
    • abnormal axon extension
      • injection of ATPgammaS into the sciatic nerve does not increase growth-associated protein-43, a marker for axonal growth, as in wild-type   (MGI Ref ID J:104694)
  • cellular phenotype
  • abnormal neuron differentiation
    • treatment with ATPgammaS in the presence of nerve growth factor does not increase dorsal root ganglion neuronal differentiation as in wild-type   (MGI Ref ID J:104694)
    • abnormal axon extension
      • injection of ATPgammaS into the sciatic nerve does not increase growth-associated protein-43, a marker for axonal growth, as in wild-type   (MGI Ref ID J:104694)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Channel and Transporter Defects

Internal/Organ Research
Kidney Defects

Neurobiology Research
Channel and Transporter Defects

Research Tools
Immunology, Inflammation and Autoimmunity Research
      genes regulating susceptibility to infectious disease and endotoxin

Sensorineural Research
Nociception
Taste Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol P2ry2tm1Bhk
Allele Name targeted mutation 1, Beverly Koller
Allele Type Targeted (knock-out)
Common Name(s) P2Y2-R-; P2Y2-; P2ry2tm1Bouc;
Mutation Made By Beverly Koller,   Univ. of North Carolina at Chapel Hill
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name P2ry2, purinergic receptor P2Y, G-protein coupled 2
Chromosome 7
Gene Common Name(s) HP2U; P2RU1; P2U; P2U1; P2UR; P2Y2; P2Y2R;
Molecular Note A neomycin selection cassette replaced a genomic fragment corresponding to base pairs 552 - 1149 of the published cDNA. Northern blot analysis on RNA derived from kidney of homozygous mice confirmed that no detectable transcript is expressed from this allele. [MGI Ref ID J:57617]

Genotyping

Genotyping Information

Genotyping Protocols

P2ry2tm1Bhk, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Homolya L; Watt WC; Lazarowski ER; Koller BH; Boucher RC. 1999. Nucleotide-regulated calcium signaling in lung fibroblasts and epithelial cells from normal and P2Y(2) receptor (-/-) mice. J Biol Chem 274(37):26454-60. [PubMed: 10473605]  [MGI Ref ID J:57617]

Additional References

P2ry2tm1Bhk related

Arthur DB; Akassoglou K; Insel PA. 2005. P2Y2 receptor activates nerve growth factor/TrkA signaling to enhance neuronal differentiation. Proc Natl Acad Sci U S A 102(52):19138-43. [PubMed: 16365320]  [MGI Ref ID J:104694]

Beldi G; Wu Y; Sun X; Imai M; Enjyoji K; Csizmadia E; Candinas D; Erb L; Robson SC. 2008. Regulated catalysis of extracellular nucleotides by vascular CD39/ENTPD1 is required for liver regeneration. Gastroenterology 135(5):1751-60. [PubMed: 18804472]  [MGI Ref ID J:145637]

Chen Y; Corriden R; Inoue Y; Yip L; Hashiguchi N; Zinkernagel A; Nizet V; Insel PA; Junger WG. 2006. ATP release guides neutrophil chemotaxis via P2Y2 and A3 receptors. Science 314(5806):1792-5. [PubMed: 17170310]  [MGI Ref ID J:116274]

Cicko S; Lucattelli M; Muller T; Lommatzsch M; De Cunto G; Cardini S; Sundas W; Grimm M; Zeiser R; Durk T; Zissel G; Boeynaems JM; Sorichter S; Ferrari D; Di Virgilio F; Virchow JC; Lungarella G; Idzko M. 2010. Purinergic receptor inhibition prevents the development of smoke-induced lung injury and emphysema. J Immunol 185(1):688-97. [PubMed: 20519655]  [MGI Ref ID J:161621]

Cressman VL; Lazarowski E; Homolya L; Boucher RC; Koller BH; Grubb BR. 1999. Effect of loss of P2Y(2) receptor gene expression on nucleotide regulation of murine epithelial Cl(-) transport. J Biol Chem 274(37):26461-8. [PubMed: 10473606]  [MGI Ref ID J:57618]

Dong X; Smoll EJ; Ko KH; Lee J; Chow JY; Kim HD; Insel PA; Dong H. 2009. P2Y receptors mediate Ca2+ signaling in duodenocytes and contribute to duodenal mucosal bicarbonate secretion. Am J Physiol Gastrointest Liver Physiol 296(2):G424-32. [PubMed: 19074643]  [MGI Ref ID J:146373]

Ehre C; Zhu Y; Abdullah LH; Olsen J; Nakayama KI; Nakayama K; Messing RO; Davis CW. 2007. nPKCepsilon, a P2Y2-R downstream effector in regulated mucin secretion from airway goblet cells. Am J Physiol Cell Physiol 293(5):C1445-54. [PubMed: 17728398]  [MGI Ref ID J:145117]

Elliott MR; Chekeni FB; Trampont PC; Lazarowski ER; Kadl A; Walk SF; Park D; Woodson RI; Ostankovich M; Sharma P; Lysiak JJ; Harden TK; Leitinger N; Ravichandran KS. 2009. Nucleotides released by apoptotic cells act as a find-me signal to promote phagocytic clearance. Nature 461(7261):282-6. [PubMed: 19741708]  [MGI Ref ID J:152837]

Geary C; Akinbi H; Korfhagen T; Fabre JE; Boucher R; Rice W. 2005. Increased susceptibility of purinergic receptor-deficient mice to lung infection with Pseudomonas aeruginosa. Am J Physiol Lung Cell Mol Physiol 289(5):L890-5. [PubMed: 16024720]  [MGI Ref ID J:115441]

Guns PJ; Van Assche T; Fransen P; Robaye B; Boeynaems JM; Bult H. 2006. Endothelium-dependent relaxation evoked by ATP and UTP in the aorta of P2Y2-deficient mice. Br J Pharmacol 147(5):569-74. [PubMed: 16415908]  [MGI Ref ID J:135769]

Hanley PJ; Kronlage M; Kirschning C; del Rey A; Di Virgilio F; Leipziger J; Chessell IP; Sargin S; Filippov MA; Lindemann O; Mohr S; Konigs V; Schillers H; Bahler M; Schwab A. 2012. Transient P2X7 receptor activation triggers macrophage death independent of Toll-like receptors 2 and 4, caspase-1, and pannexin-1 proteins. J Biol Chem 287(13):10650-63. [PubMed: 22235111]  [MGI Ref ID J:183298]

Huang YA; Dando R; Roper SD. 2009. Autocrine and paracrine roles for ATP and serotonin in mouse taste buds. J Neurosci 29(44):13909-18. [PubMed: 19890001]  [MGI Ref ID J:158296]

Kiefmann R; Islam MN; Lindert J; Parthasarathi K; Bhattacharya J. 2009. Paracrine purinergic signaling determines lung endothelial nitric oxide production. Am J Physiol Lung Cell Mol Physiol 296(6):L901-10. [PubMed: 19304909]  [MGI Ref ID J:149610]

Kim HJ; Ajit D; Peterson TS; Wang Y; Camden JM; Gibson Wood W; Sun GY; Erb L; Petris M; Weisman GA. 2012. Nucleotides released from Abeta(1)(-)(4)(2) -treated microglial cells increase cell migration and Abeta(1)(-)(4)(2) uptake through P2Y(2) receptor activation. J Neurochem 121(2):228-38. [PubMed: 22353164]  [MGI Ref ID J:184286]

Kouzaki H; Iijima K; Kobayashi T; O'Grady SM; Kita H. 2011. The Danger Signal, Extracellular ATP, Is a Sensor for an Airborne Allergen and Triggers IL-33 Release and Innate Th2-Type Responses. J Immunol 186(7):4375-87. [PubMed: 21357533]  [MGI Ref ID J:170695]

Kronlage M; Song J; Sorokin L; Isfort K; Schwerdtle T; Leipziger J; Robaye B; Conley PB; Kim HC; Sargin S; Schon P; Schwab A; Hanley PJ. 2010. Autocrine purinergic receptor signaling is essential for macrophage chemotaxis. Sci Signal 3(132):ra55. [PubMed: 20664064]  [MGI Ref ID J:185405]

Lammermann T; Afonso PV; Angermann BR; Wang JM; Kastenmuller W; Parent CA; Germain RN. 2013. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature 498(7454):371-5. [PubMed: 23708969]  [MGI Ref ID J:198733]

Malin SA; Davis BM; Koerber HR; Reynolds IJ; Albers KM; Molliver DC. 2008. Thermal nociception and TRPV1 function are attenuated in mice lacking the nucleotide receptor P2Y(2). Pain 138(3):484-96. [PubMed: 18343036]  [MGI Ref ID J:144263]

Mamenko M; Zaika O; Jin M; O'Neil RG; Pochynyuk O. 2011. Purinergic activation of Ca2+-permeable TRPV4 channels is essential for mechano-sensitivity in the aldosterone-sensitive distal nephron. PLoS One 6(8):e22824. [PubMed: 21850238]  [MGI Ref ID J:176515]

Matos JE; Robaye B; Boeynaems JM; Beauwens R; Leipziger J. 2005. K+ secretion activated by luminal P2Y2 and P2Y4 receptors in mouse colon. J Physiol 564(Pt 1):269-79. [PubMed: 15718265]  [MGI Ref ID J:112547]

Pochynyuk O; Bugaj V; Rieg T; Insel PA; Mironova E; Vallon V; Stockand JD. 2008. Paracrine Regulation of the Epithelial Na+ Channel in the Mammalian Collecting Duct by Purinergic P2Y2 Receptor Tone. J Biol Chem 283(52):36599-607. [PubMed: 18981175]  [MGI Ref ID J:143916]

Pochynyuk O; Rieg T; Bugaj V; Schroth J; Fridman A; Boss GR; Insel PA; Stockand JD; Vallon V. 2010. Dietary Na+ inhibits the open probability of the epithelial sodium channel in the kidney by enhancing apical P2Y2-receptor tone. FASEB J 24(6):2056-65. [PubMed: 20097874]  [MGI Ref ID J:162364]

Rieg T; Gerasimova M; Boyer JL; Insel PA; Vallon V. 2011. P2Y receptor activation decreases blood pressure and increases renal Na excretion. Am J Physiol Regul Integr Comp Physiol 301(2):R510-8. [PubMed: 21613580]  [MGI Ref ID J:178659]

Rowlands DJ; Islam MN; Das SR; Huertas A; Quadri SK; Horiuchi K; Inamdar N; Emin MT; Lindert J; Ten VS; Bhattacharya S; Bhattacharya J. 2011. Activation of TNFR1 ectodomain shedding by mitochondrial Ca2+ determines the severity of inflammation in mouse lung microvessels. J Clin Invest 121(5):1986-99. [PubMed: 21519143]  [MGI Ref ID J:173932]

Schumacher D; Strilic B; Sivaraj KK; Wettschureck N; Offermanns S. 2013. Platelet-Derived Nucleotides Promote Tumor-Cell Transendothelial Migration and Metastasis via P2Y2 Receptor. Cancer Cell 24(1):130-7. [PubMed: 23810565]  [MGI Ref ID J:199146]

Stockand JD; Mironova E; Bugaj V; Rieg T; Insel PA; Vallon V; Peti-Peterdi J; Pochynyuk O. 2010. Purinergic inhibition of ENaC produces aldosterone escape. J Am Soc Nephrol 21(11):1903-11. [PubMed: 20813869]  [MGI Ref ID J:185905]

Sun S; Xia S; Ji Y; Kersten S; Qi L. 2012. The ATP-P2X7 signaling axis is dispensable for obesity-associated inflammasome activation in adipose tissue. Diabetes 61(6):1471-8. [PubMed: 22415881]  [MGI Ref ID J:196855]

Vallon V; Rieg T. 2011. Regulation of renal NaCl and water transport by the ATP/UTP/P2Y2 receptor system. Am J Physiol Renal Physiol 301(3):F463-75. [PubMed: 21715471]  [MGI Ref ID J:175906]

Vanderstocken G; Van de Paar E; Robaye B; di Pietrantonio L; Bondue B; Boeynaems JM; Desmecht D; Communi D. 2012. Protective role of P2Y2 receptor against lung infection induced by pneumonia virus of mice. PLoS One 7(11):e50385. [PubMed: 23185614]  [MGI Ref ID J:195578]

Yu N; Erb L; Shivaji R; Weisman GA; Seye CI. 2008. Binding of the P2Y2 nucleotide receptor to filamin A regulates migration of vascular smooth muscle cells. Circ Res 102(5):581-8. [PubMed: 18202316]  [MGI Ref ID J:147657]

Zaika O; Zhang J; Shapiro MS. 2011. Functional role of M-type (KCNQ) K(+) channels in adrenergic control of cardiomyocyte contraction rate by sympathetic neurons. J Physiol 589(Pt 10):2559-68. [PubMed: 21486761]  [MGI Ref ID J:185252]

Zhang Y; Li L; Kohan DE; Ecelbarger CM; Kishore BK. 2013. Attenuation of lithium-induced natriuresis and kaliuresis in P2Y(2) receptor knockout mice. Am J Physiol Renal Physiol 305(3):F407-16. [PubMed: 23739592]  [MGI Ref ID J:200828]

Zhang Y; Listhrop R; Ecelbarger CM; Kishore BK. 2011. Renal sodium transporter/channel expression and sodium excretion in P2Y2 receptor knockout mice fed a high-NaCl diet with/without aldosterone infusion. Am J Physiol Renal Physiol 300(3):F657-68. [PubMed: 21190950]  [MGI Ref ID J:169415]

Zhang Y; Pop IL; Carlson NG; Kishore BK. 2012. Genetic deletion of the P2Y2 receptor offers significant resistance to development of lithium-induced polyuria accompanied by alterations in PGE2 signaling. Am J Physiol Renal Physiol 302(1):F70-7. [PubMed: 21975874]  [MGI Ref ID J:180094]

Zhang Y; Sands JM; Kohan DE; Nelson RD; Martin CF; Carlson NG; Kamerath CD; Ge Y; Klein JD; Kishore BK. 2008. Potential role of purinergic signaling in urinary concentration in inner medulla: insights from P2Y2 receptor gene knockout mice. Am J Physiol Renal Physiol 295(6):F1715-24. [PubMed: 18829742]  [MGI Ref ID J:142804]

del Rey A; Renigunta V; Dalpke AH; Leipziger J; Matos JE; Robaye B; Zuzarte M; Kavelaars A; Hanley PJ. 2006. Knock-out mice reveal the contributions of P2Y and P2X receptors to nucleotide-induced Ca2+ signaling in macrophages. J Biol Chem 281(46):35147-55. [PubMed: 16980298]  [MGI Ref ID J:117254]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   29-DEC-09
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $195.00Female or MaleHomozygous for P2ry2tm1Bhk  
Price per Pair (US dollars $)Pair Genotype
$390.00Homozygous for P2ry2tm1Bhk x Homozygous for P2ry2tm1Bhk  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $253.50Female or MaleHomozygous for P2ry2tm1Bhk  
Price per Pair (US dollars $)Pair Genotype
$507.00Homozygous for P2ry2tm1Bhk x Homozygous for P2ry2tm1Bhk  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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