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| These TetO-Kir2.1-IRES-tauLacZ transgenic mice express a Kcnj2 cDNA and a tau-β-galactosidase cassette under the control of tet-operator. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 02-MAR-12 Mating System Hemizygote x Noncarrier (Female x Male) 02-MAR-12 Species laboratory mouse Generation N6pN1+ (05-JUN-12)
Generation DefinitionsDonating Investigator Joseph Gogos, Columbia University Description
These mice express a potassium inwardly-rectifying channel, subfamily J, member 2 (Kcnj2) cDNA and a tau-β-galactosidase (lacZ) cassette under the direction of tetracycline operator. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of KCNJ2 and lacZ may be regulated with the tetracycline analog doxycycline in the double mutant offspring. Hemizygous mice are viable and fertile. For example, when bred to mice expressing olfactory marker protein (Omp)-tTA, the olfactory sensory neurons in bigenic offspring express KCNJ2 and the taulacZ fusion protein by virtue of IRES-mediated co-translation after dox treatment. Overexpression KCNJ2 hyperpolarizes neurons and inhibits the firing of action potentials, disrupting the formation of the olfactory map. These neurons can be revealed by histochemistry of beta-galactosidase enzymatic activity. These mice may be useful for studying the establishment and maintenance of olfactory receptor specific neural activity.When bred to a strain expressing tTA in the neurons of the olfactory bulb (see Stock No. 017754 for example), this mutant mouse strain may be useful in studies of the olfactory sensory map.
Development
The TetO-Kir2.1-IRES-tauLacZ transgene was designed with, from 5' to 3', the tetracycline operator (tetO or tet-operator), a minimal promoter (Pmin), a potassium inwardly-rectifying channel, subfamily J, member 2 (Kcnj2) cDNA, an internal ribosome entry site (IRES), a tau- β-galactosidase (lacZ), and an SV40 polyadenylation sequence. This transgene was microinjected into (C57BL/6 and CBA)F1 oocytes. Mice from founder line 1 were bred to C57BL/6 mice for at least 6 generations to establish the colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred with C57BL/6J hybrid mice (Stock No. 000664) for at least one generation.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Kcnj2
005057 FVB.129-Kcnj2tm1Swz/J View Strains carrying other alleles of Kcnj2 (1 strain)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Andersen Cardiodysrhythmic Periodic Paralysis (KCNJ2)
Atrial Fibrillation, Familial, 9; ATFB9 (KCNJ2)
Short Qt Syndrome 3; SQT3 (KCNJ2)
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Omptm1(tTA)Gogo/Omp+ Tg(tetO-Kcnj2,lacZ)1Gogo/?
involves: 129 * C57BL/6 * CBA
- nervous system phenotype
- abnormal olfactory bulb morphology
- following doxycycline treatment, organization of the glomeruli in the dorsal part of the bulb remains disorganized, thinly innervated, necklace glomeruli are absent, and nerves that enter the bulb coalesce into a giant glomerulus-like structure (MGI Ref ID J:124299)
- abnormal olfactory nerve morphology
This mouse can be used to support research in many areas including:
Neurobiology Research
lacZ expression in neural tissue
Tet Expression System
tTA/rtTA Responsive Strains
Research Tools
lacZ Expression
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(tetO-Kcnj2,lacZ)1Gogo | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Joseph Gogos | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | TRE-KZ; tetO-Kir2.1.ires.LacZ; teto-Kir2.1-IRES-tau-lacZ; | ||
| Mutation Made By | Joseph Gogos, Columbia University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | When bred to mice expressing a tetracycline transactivator (tTA) or reverse transactivator (rtTA), doxycycline induction results in the expression of lacZ in transactivator expressing tissues of the offspring. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Expressed Gene | Kcnj2, potassium inwardly-rectifying channel, subfamily J, member 2, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | In this transgene the tetracyclin promoter is fused to the open reading frame of Kcnj2-IRES-tau-lacZ. This transgene is inducible and when used with Omptm1(tTA)1Gogo is expressed in 80% of olfactory neurons. [MGI Ref ID J:124299] | ||
| Gene Symbol and Name | Tg(tetO-Kcnj2,lacZ)1Gogo, transgene insertion 1, Joseph Gogos | ||
| Chromosome | UN | ||
| Gene Common Name(s) | teto-Kir2.1-IRES-tau-lacZ; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-Kcnj2,lacZ)1Gogo, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Yu CR; Power J; Barnea G; O'Donnell S; Brown HE; Osborne J; Axel R; Gogos JA. 2004. Spontaneous neural activity is required for the establishment and maintenance of the olfactory sensory map. Neuron 42(4):553-66. [PubMed: 15157418] [MGI Ref ID J:124299]
Additional References
Tg(tetO-Kcnj2,lacZ)1Gogo relatedDe Marco Garcia NV; Karayannis T; Fishell G. 2011. Neuronal activity is required for the development of specific cortical interneuron subtypes. Nature 472(7343):351-5. [PubMed: 21460837] [MGI Ref ID J:171376]
Tasic B; Miyamichi K; Hippenmeyer S; Dani VS; Zeng H; Joo W; Zong H; Chen-Tsai Y; Luo L. 2012. Extensions of MADM (Mosaic Analysis with Double Markers) in Mice PLoS ONE 7(3):e33332. [PubMed: 22479386] [MGI Ref ID J:181830]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX18
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6 inbred mice (Stock No. 000664). Mating System Noncarrier x Hemizygote (Female x Male) 02-MAR-12 Hemizygote x Noncarrier (Female x Male) 02-MAR-12 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(tetO-Kcnj2,lacZ)1Gogo
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(tetO-Kcnj2,lacZ)1Gogo x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(tetO-Kcnj2,lacZ)1Gogo Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(tetO-Kcnj2,lacZ)1Gogo
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(tetO-Kcnj2,lacZ)1Gogo x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(tetO-Kcnj2,lacZ)1Gogo Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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