Strain Name:

B6.129P2-Polbtm1Rsky/J

Stock Number:

009154

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These polβflox (or polBflox) mutant mice possess loxP sites on either side of exon 1 of the Polb (polymerase (DNA directed), beta) gene and may be useful in generating conditional mutations for studying DNA repair.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129P2-Polbtm1Hua/J    (Changed: 30-OCT-09 )
Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN6+N1F2pN1
Generation Definitions
 
Donating Investigator Klaus Rajewsky,   Max-Delbruck-Ctr. for Molecular Medicine

Description
These polβflox (or polBflox) mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying DNA repair.

Development
A loxP site flanked targeting vector containing hygromycin resistance and thymidine kinase genes utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 1 of the targeted gene, and another loxP site was inserted approximately 2 kb upstream of exon 1. This construct was electroporated into 129P2/OlaHsd derived E14.1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exon 1 were injected in recipient blastocysts. Resulting chimeric animals were backcrossed to wildtype C57BL/6 mice. The mice were then backcrossed to C57BL/6 for at least 5 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ once to establish the colony.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Other
      DNA Repair

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Polbtm1Rsky
Allele Name targeted mutation 1, Klaus Rajewsky
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) BT14; PolBflox; PolBetaflox; Polbtm1Hua;
Mutation Made By Klaus Rajewsky,   Max-Delbruck-Ctr. for Molecular Medicine
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Polb, polymerase (DNA directed), beta
Chromosome 8
Gene Common Name(s) A430088C08Rik; Pol beta; RIKEN cDNA A430088C08 gene;
Molecular Note Single loxP sites were left flanking exon 1 and 2 kb of upstream sequence including the promoter after a neomycin selection cassette was excised via the in vitro expression of cre recombinase. [MGI Ref ID J:67942]

Genotyping

Genotyping Information

Genotyping Protocols

Polbtm1Hua, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Gu H; Marth JD; Orban PC; Mossmann H; Rajewsky K. 1994. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265(5168):103-6. [PubMed: 8016642]  [MGI Ref ID J:67942]

Additional References

Polbtm1Rsky related

Betz UA; Vosshenrich CA; Rajewsky K; Muller W. 1996. Bypass of lethality with mosaic mice generated by Cre-loxP-mediated recombination. Curr Biol 6(10):1307-16. [PubMed: 8939573]  [MGI Ref ID J:67926]

Cabelof DC; Raffoul JJ; Nakamura J; Kapoor D; Abdalla H; Heydari AR. 2004. Imbalanced base excision repair in response to folate deficiency is accelerated by polymerase beta haploinsufficiency. J Biol Chem 279(35):36504-13. [PubMed: 15218023]  [MGI Ref ID J:92544]

Clausen BE; Burkhardt C; Reith W; Renkawitz R; Forster I. 1999. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res 8(4):265-77. [PubMed: 10621974]  [MGI Ref ID J:67924]

Kidane D; Jonason AS; Gorton TS; Mihaylov I; Pan J; Keeney S; de Rooij DG; Ashley T; Keh A; Liu Y; Banerjee U; Zelterman D; Sweasy JB. 2010. DNA polymerase beta is critical for mouse meiotic synapsis. EMBO J 29(2):410-23. [PubMed: 20019666]  [MGI Ref ID J:156442]

Schwenk F; Kuhn R; Angrand PO; Rajewsky K; Stewart AF. 1998. Temporally and spatially regulated somatic mutagenesis in mice. Nucleic Acids Res 26(6):1427-32. [PubMed: 9490788]  [MGI Ref ID J:69333]

Seibler J; Zevnik B; Kuter-Luks B; Andreas S; Kern H; Hennek T; Rode A; Heimann C; Faust N; Kauselmann G; Schoor M; Jaenisch R; Rajewsky K; Kuhn R; Schwenk F. 2003. Rapid generation of inducible mouse mutants. Nucleic Acids Res 31(4):e12. [PubMed: 12582257]  [MGI Ref ID J:126518]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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