Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse   Donating Investigator Paul Anderson,   Harvard Medical School

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by immunoblot analysis of embryonic fibroblast lysates. Homozygotes are more susceptible to LPS-induced endotoxin shock than wildtype controls and develop mild arthritis. Macrophages derived from these animals produce significantly more TNF-alpha than wild-type controls. Mutant mice exhibit altered polysome disassembly. This mutant mouse strain may be useful in studies of stress-induced translational arrest and silencing, inflammation, and arthritis.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt parts of intron 3 and exon 4. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to BALB/c mice, and then backcrossed to BALB/c for 3 generations. The mice were then backcrossed to C57BL/6 for 14 generations before arriving at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Tia1^tm1Andp/Tia1^tm1Andp

        B6.Cg-Tia1^tm1Andp

• immune system phenotype
• arthritis
  • homozygotes develop mild arthritis   (MGI Ref ID J:88443)
  • swollen joints display minimal display inflammatory pannus and rare bony erosions   (MGI Ref ID J:88443)
• skeleton phenotype
• arthritis
  • homozygotes develop mild arthritis   (MGI Ref ID J:88443)
  • swollen joints display minimal display inflammatory pannus and rare bony erosions   (MGI Ref ID J:88443)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tia1^tm1Andp/Tia1^tm1Andp

        C.129S2-Tia1^tm1Andp

• mortality/aging
• partial postnatal lethality
  • approximately 50% of homozygotes die between E16.5 and 3 weeks   (MGI Ref ID J:88770)
  • survivors appear normal and live up to 2 years   (MGI Ref ID J:88770)
• hematopoietic system phenotype
• *normal* hematopoietic system phenotype
  • major hematopoietic lineages are in normal proportions   (MGI Ref ID J:88770)
• immune system phenotype
• *normal* immune system phenotype
  • the thymus is normal   (MGI Ref ID J:88770)
• • abnormal macrophage physiology
    • in macrophages from homozygotes lipopolysaccharide-induced expression of TNF- alpha and IL-6 is significantly increased   (MGI Ref ID J:88770)
  • increased susceptibility to endotoxin shock
    • mutants are more susceptible to lipopolysaccharide-induced endotoxin shock   (MGI Ref ID J:88770)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Transcriptional Regulation

Immunology, Inflammation and Autoimmunity Research
Inflammation
      rheumatoid arthritis

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tia1tm1Andp
Allele Name		targeted mutation 1, Paul Anderson
Allele Type		Targeted (knock-out)
Common Name(s)		TIA-1-;
Mutation Made By		Paul Anderson,   Harvard Medical School
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Tia1, cytotoxic granule-associated RNA binding protein 1
Chromosome		6
Gene Common Name(s)		2310050N03Rik; AI256674; EST AI256674; RIKEN cDNA 2310050N03 gene; TIA-1; WDM; mTIA-1;
Molecular Note		The gene was disrupted by replacement of parts of intron 3 and exon 4 of the genomic DNA with a neomycin cassette via homologous recombination. Southern blot analysis confirmed recombination and immunoprecipitation demonstrated that no detectable protein is produced from this allele. [MGI Ref ID J:88770]

Genotyping

Genotyping Information

Genotyping Protocols

Tia1^tm1Andp, Separated PCR
Tia1^tm1Andp, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Piecyk M; Wax S; Beck AR; Kedersha N; Gupta M; Maritim B; Chen S; Gueydan C; Kruys V; Streuli M; Anderson P. 2000. TIA-1 is a translational silencer that selectively regulates the expression of TNF-alpha. EMBO J 19(15):4154-63. [PubMed: 10921895]  [MGI Ref ID J:88770]

Additional References

Tia1^tm1Andp related

Katsanou V; Papadaki O; Milatos S; Blackshear PJ; Anderson P; Kollias G; Kontoyiannis DL. 2005. HuR as a negative posttranscriptional modulator in inflammation. Mol Cell 19(6):777-89. [PubMed: 16168373]  [MGI Ref ID J:102638]

Yamasaki S; Stoecklin G; Kedersha N; Simarro M; Anderson P. 2007. T-cell intracellular antigen-1 (TIA-1)-induced translational silencing promotes the decay of selected mRNAs. J Biol Chem 282(41):30070-7. [PubMed: 17711853]  [MGI Ref ID J:126789]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

Terms of Use

General Terms and Conditions

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Contact information

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