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| These transgenic mice express the mouse interferon-gamma gene regulated by a tetracycline-responsive promoter and may be useful for studying antiviral responses, immune surveillance, inhibiting cellular proliferation, and tumor suppression. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N15pN1
Generation DefinitionsDonating Investigator Brian Popko, University of Chicago Description
The donating investigator reports that homozygous mice are viable and fertile. These TRE/IFN-γ transgenic mice have expression of mouse interferon-gamma (IFN-γ) regulated by the tetracycline-responsive promoter (tetO [also called tetracycline-responsive element (TRE or tet-operator)] fused to the human cytomegalovirus minimal promoter). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of IFN-γ may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. Because interferon-gamma (IFN-γ) is a pleiotropic cytokine secreted by activated T-lymphocytes and natural killer cells, these line 184 TRE/IFN-γ mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of IFN-γ for studying antiviral responses, immune surveillance, inhibiting cellular proliferation, and tumor suppression.For example, these TRE/IFN-γ transgenic mice may be bred with GFAP-tTA transgenic mice (Stock No. 005964) for studying medulloblastoma in the developing cerebellum.
Development
The TRE/IFN-γ transgene was designed with the PhCMV*-1 tetracycline-responsive promoter upstream of a mouse IFN-γ cDNA sequence. The PhCMV*-1 tetracycline-responsive promoter contains the human cytomegalovirus (hCMV) minimal promoter fused to the tet-operator (tetO; also called tetracycline-responsive element [TRE]). The IFN-γ cDNA is flanked by 40 bp of 5' and 57 bp of 3' UTR sequences and includes the translation start and stop signals. The sequences that confer IFN-γ mRNA instability have been eliminated from the IFN-γ cDNA sequence in an effort to increase steady-state mRNA levels derived from the transgene. This 2.3 kb transgene was microinjected into fertilized B6D2F1 oocytes. Mice from founder line 184 were bred with C57BL/6J mice to create the colony. The donating investigator reports that line 184 TRE/IFN-γ mice were backcrossed to C57BL/6J for approximately 15 generations prior to arrival at The Jackson Laboratory. Upon arrival, transgenic mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish this colony. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ifng
002287 B6.129S7-Ifngtm1Ts/J 002286 C.129S7(B6)-Ifngtm1Ts/J 008228 C3.129S7(B6)-Ifngtm1Ts/J 002575 NOD.129S7(B6)-Ifngtm1Ts/DvsJ View Strains carrying other alleles of Ifng (4 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(GFAP-tTA)110Pop/0 Tg(tetO-Ifng)184Pop/0
involves: 129S6/SvEvTac * C57BL/6 * DBA/2 * Swiss WebsterThis mouse can be used to support research in many areas including:
Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines
Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules
Neurobiology Research
Tet Expression System
tTA/rtTA Responsive Strains
Research Tools
Cancer Research
Tetop Tet System
Cardiovascular Research
Tetop Tet System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Immunology and Inflammation Research
NK Cell Deficiency
T cell deficiency
Neurobiology Research
Tetop Tet System
Reproductive Biology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Responsive Strains
Virology Research
B and T Cell Deficiency
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(tetO-Ifng)184Pop | ||
|---|---|---|---|
| Allele Name | transgene insertion 184, Brian Popko | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TRE/IFN-gamma 184; TRE/IFN-gamma line 184; line 184 TRE/IFN-g; line 184 TRE/IFN-gamma; line 184 TRE/IFN-gamma , line 184 TRE/IFN-g , TRE/IFN-gamma 184 , TRE/IFN-gamma line 184 , line 184 TRE/IFNg; line 184 TRE/IFNg; | ||
| Mutation Made By | Brian Popko, University of Chicago | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Expressed Gene | Ifng, interferon gamma, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The TRE/IFN-gamma transgene was designed with the PhCMV*-1 tetracycline-responsive promoter upstream of a mouse IFN-g cDNA sequence. The PhCMV*-1 tetracycline-responsive promoter contains the human cytomegalovirus (hCMV) minimal promoter fused to the tet-operator (tetO; TRE). The IFN-g cDNA is flanked by 40 bp of 5' and 57 bp of 3' UTR sequences and includes the translation start and stop signals. The sequences that confer IFN-g mRNA instability have been eliminated from the IFN-g cDNA sequence in an effort to increase steady-state mRNA levels derived from the transgene. This 2.3 kb transgene was microinjected into fertilized B6D2F1 oocytes. Founder line 184 was established. [MGI Ref ID J:94092] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-Ifng), Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Lin W; Kemper A; McCarthy KD; Pytel P; Wang JP; Campbell IL; Utset MF; Popko B. 2004. Interferon-gamma induced medulloblastoma in the developing cerebellum. J Neurosci 24(45):10074-83. [PubMed: 15537876] [MGI Ref ID J:94092]
Additional References
Tg(tetO-Ifng)184Pop relatedLin W; Harding HP; Ron D; Popko B. 2005. Endoplasmic reticulum stress modulates the response of myelinating oligodendrocytes to the immune cytokine interferon-gamma. J Cell Biol 169(4):603-12. [PubMed: 15911877] [MGI Ref ID J:99655]
Wang J; Lin W; Popko B; Campbell IL. 2004. Inducible production of interferon-gamma in the developing brain causes cerebellar dysplasia with activation of the Sonic hedgehog pathway. Mol Cell Neurosci 27(4):489-96. [PubMed: 15555926] [MGI Ref ID J:109838]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) mice from the colony or C57BL/6J inbred mice (Stock No. 000664). The donating investigator reports that homozygous mice are viable and fertile.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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