Description

Strain Information

Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse   Donating Investigator Brian Popko,   University of Chicago

Description
The donating investigator reports that homozygous mice are viable and fertile. These TRE/IFN-γ transgenic mice have expression of mouse interferon-gamma (IFN-γ) regulated by the tetracycline-responsive promoter (tetO [also called tetracycline-responsive element (TRE or tet-operator)] fused to the human cytomegalovirus minimal promoter). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of IFN-γ may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. Because interferon-gamma (IFN-γ) is a pleiotropic cytokine secreted by activated T-lymphocytes and natural killer cells, these line 184 TRE/IFN-γ mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of IFN-γ for studying antiviral responses, immune surveillance, inhibiting cellular proliferation, and tumor suppression.

For example, these TRE/IFN-γ transgenic mice may be bred with GFAP-tTA transgenic mice (Stock No. 005964) for studying medulloblastoma in the developing cerebellum.

Development
The TRE/IFN-γ transgene was designed with the P_hCMV*-1 tetracycline-responsive promoter upstream of a mouse IFN-γ cDNA sequence. The P_hCMV*-1 tetracycline-responsive promoter contains the human cytomegalovirus (hCMV) minimal promoter fused to the tet-operator (tetO; also called tetracycline-responsive element [TRE]). The IFN-γ cDNA is flanked by 40 bp of 5' and 57 bp of 3' UTR sequences and includes the translation start and stop signals. The sequences that confer IFN-γ mRNA instability have been eliminated from the IFN-γ cDNA sequence in an effort to increase steady-state mRNA levels derived from the transgene. This 2.3 kb transgene was microinjected into fertilized B6D2F1 oocytes. Mice from founder line 184 were bred with C57BL/6J mice to create the colony. The donating investigator reports that line 184 TRE/IFN-γ mice were backcrossed to C57BL/6J for approximately 15 generations prior to arrival at The Jackson Laboratory. Upon arrival, transgenic mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish this colony. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Tg(GFAP-tTA)110Pop/0 Tg(tetO-Ifng)184Pop/0

        involves: 129S6/SvEvTac * C57BL/6 * DBA/2 * Swiss Webster

• life span-post-weaning/aging
• *normal* life span-post-weaning/aging
  • when doxycycline treatment is stopped at E14, double transgenic offspring exhibit good survival   (MGI Ref ID J:99655)
• behavior/neurological phenotype
• ataxia
  • pups exhibit minor ataxia   (MGI Ref ID J:99655)
• tremors
  • pups exhibit minor tremor   (MGI Ref ID J:99655)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules

Neurobiology Research
Tet Expression System
      tTA/rtTA Responsive Strains

Research Tools
Cancer Research
      Tetop Tet System
Cardiovascular Research
      Tetop Tet System
Genetics Research
      Mutagenesis and Transgenesis: Tetop Tet System
Immunology and Inflammation Research
      NK Cell Deficiency
      T cell deficiency
Neurobiology Research
      Tetop Tet System
Reproductive Biology Research
      Tetop Tet System
Tet Expression Systems
      tTA/rtTA Responsive Strains

Virology Research
B and T Cell Deficiency

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(tetO-Ifng)184Pop
Allele Name		transgene insertion 184, Brian Popko
Allele Type		Transgenic (random, expressed)
Common Name(s)		TRE/IFN-gamma 184; TRE/IFN-gamma line 184; line 184 TRE/IFN-g; line 184 TRE/IFN-gamma; line 184 TRE/IFN-gamma , line 184 TRE/IFN-g , TRE/IFN-gamma 184 , TRE/IFN-gamma line 184 , line 184 TRE/IFNg; line 184 TRE/IFNg;
Mutation Made By		Brian Popko,   University of Chicago
Strain of Origin		(C57BL/6 x DBA/2)F1
Molecular Note		The TRE/IFN-gamma transgene was designed with the PhCMV*-1 tetracycline-responsive promoter upstream of a mouse IFN-g cDNA sequence. The PhCMV*-1 tetracycline-responsive promoter contains the human cytomegalovirus (hCMV) minimal promoter fused to the tet-operator (tetO; TRE). The IFN-g cDNA is flanked by 40 bp of 5' and 57 bp of 3' UTR sequences and includes the translation start and stop signals. The sequences that confer IFN-g mRNA instability have been eliminated from the IFN-g cDNA sequence in an effort to increase steady-state mRNA levels derived from the transgene. This 2.3 kb transgene was microinjected into fertilized B6D2F1 oocytes. Founder line 184 was established. [MGI Ref ID J:94092]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tetO-Ifng), Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Lin W; Kemper A; McCarthy KD; Pytel P; Wang JP; Campbell IL; Utset MF; Popko B. 2004. Interferon-gamma induced medulloblastoma in the developing cerebellum. J Neurosci 24(45):10074-83. [PubMed: 15537876]  [MGI Ref ID J:94092]

Additional References

Tg(tetO-Ifng)184Pop related

Lin W; Harding HP; Ron D; Popko B. 2005. Endoplasmic reticulum stress modulates the response of myelinating oligodendrocytes to the immune cytokine interferon-gamma. J Cell Biol 169(4):603-12. [PubMed: 15911877]  [MGI Ref ID J:99655]

Wang J; Lin W; Popko B; Campbell IL. 2004. Inducible production of interferon-gamma in the developing brain causes cerebellar dysplasia with activation of the Sonic hedgehog pathway. Mol Cell Neurosci 27(4):489-96. [PubMed: 15555926]  [MGI Ref ID J:109838]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, transgenic carrier mice may be bred with wildtype (noncarrier) mice from the colony or C57BL/6J inbred mice (Stock No. 000664). The donating investigator reports that homozygous mice are viable and fertile.

Purchasing information

Pricing, Supply Level & Notes, Controls

 

This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development and On Hold

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

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Contact information

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