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| These CDX2P9.5-G22Cre transgenic mice harbor a nuclear-localized Cre recombinase with 22 guanine nucleotide repeat between initiating methionine (ATG) codon and the remainder of the coding region altering the cre reading frame. Presumably, following a somatic mutation that results in some frameshift mutation(s) in the guanine nucleotide tract in a subset of somatic cells, resultant expression of a functional Cre recombinase is driven by a 9.5 kb human caudal type homeo box 2 (CDX2) promoter/enhancer sequence. These CDX2P9.5-G22Cre transgenic mice may be useful as a somatic mutation reporter strain and/or to generate conditional mutations in colorectal epithelial cells. | |||||||||||||||||||
- Description
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- Genes & alleles
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Eric Fearon, University of Michigan Medical School Description
Mice hemizygous for the CDX2P9.5-G22Cre transgene are viable and fertile. The 9.5 kb human caudal type homeo box 2 (CDX2) promoter/enhancer sequence is prevented from driving nuclear-localized Cre recombinase expression as a 22 guanine nucleotide repeat tract between initiating methionine (ATG) codon and the remainder of the coding region alters the cre reading frame. Presumably, following a somatic mutation that results in some frameshift mutation(s) in the guanine nucleotide tract in a subset of somatic cells, resultant expression of a functional Cre recombinase is observed predominantly in colonic epithelium during late gestation and in adult tissues. Specifically, mosaic Cre recombinase expression would be observed in epithelium from the distal ileum and cecum, and mainly the proximal and distal colon from the crypt base to the luminal surface. Cre recombinase expression may also be observed in a few caudal-derived cells during early development. When these transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination is expected to result in deletion of the floxed sequences in these tissues of the offspring that reactivate cre expression. These CDX2P9.5-G22Cre transgenic mice may be useful as a somatic mutation reporter strain and/or to generate conditional mutations in colorectal epithelial cells.Development
The CDX2P9.5-G22Cre transgene was designed with a 9.5 kb promoter fragment from the 5' flanking sequences of human caudal type homeo box 2 (CDX2) gene, a nuclear-localized Cre recombinase gene modified with a 22 guanine nucleotide repeat tract between its initiating methionine (ATG) codon and the remainder of the Cre-coding region, and a bovine growth hormone polyadenylation cassette. This transgene was injected into (C57BL/6J x SJL/J) hybrid cells. Transgenic founders (line 189) were found to have approximately 80 copies of the transgene and were bred to C57BL/6J mice. The donating investigator reports that this strain was maintained by breeding hemizygous mice with C57BL/6J inbred mice for 7 generations prior to arrival at The Jackson Laboratory. Upon arrival, CDX2P9.5-G22Cre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. The Y chromosome may not have been fixed to the C57BL/6 genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of CDX2
009350 B6.Cg-Tg(CDX2-cre)101Erf/J View Strains carrying other alleles of CDX2 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedInternal/Organ Research
Gastrointestinal Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CDX2-cre*)189Erf | ||
|---|---|---|---|
| Allele Name | transgene insertion 189, Eric R Fearon | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | CDX2P9.5-G22Cre; | ||
| Mutation Made By | Eric Fearon, University of Michigan Medical School | ||
| Strain of Origin | (C57BL/6J x SJL/J) | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | CDX2, caudal type homeobox 2, human | ||
| Driver Note | CDX2 | ||
| Molecular Note | The CDX2P9.5-G22Cre transgene was designed with a 9.5 kb promoter fragment from the 5' flanking sequences of human caudal type homeo box 2 (CDX2) gene, a nuclear-localized Cre recombinase gene modified with a 22 guanine nucleotide repeat tract between its initiating methionine (ATG) codon and the remainder of the Cre-coding region, and a bovine growth hormone polyadenylation cassette. This transgene was injected into (C57BL/6J x SJL/J) hybrid cells. Transgenic founders (line 189) were found to have approximately 80 copies of the transgene and were bred to C57BL/6J mice. [MGI Ref ID J:148161] | ||
| Gene Symbol and Name | Tg(CDX2-cre*)189Erf, transgene insertion 189, Eric R Fearon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | CDX2P9.5-G22Cre; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Akyol A; Hinoi T; Feng Y; Bommer GT; Glaser TM; Fearon ER. 2008. Generating somatic mosaicism with a Cre recombinase-microsatellite sequence transgene. Nat Methods 5(3):231-3. [PubMed: 18264107] [MGI Ref ID J:148161]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygotes may be bred with noncarrier (wildtype) siblings or C57BL/6J inbred mice.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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