Description

Strain Information

Type Inbred Strain; Additional information on Inbred Strains. Visit our online Nomenclature tutorial. Mating System Sibling x Sibling         (Female x Male)   24-NOV-09 Species laboratory mouse H2 Haplotype H2k (also called H-2k or H2k) Generation F?+F4 (30-JUN-11) Generation Definitions   Donating Investigator Marcia McDuffie,   University of Virginia Dept of Medicine

Important Note
These SAMP1 mice have significant breeding difficulties. Please see the JAXMice Health & Husbandry section for the SAMP1 mice Colony Maintenance / Breeding & Husbandry description.

Description
SAMP1/YitFcs (also called SAMP1/YitFc or SAMP1/Fc) mice develop a spontaneous ileitis that is similar in many features to human Crohn's disease. Development of ileitis is accelerated by the presence of luminal bacteria and is characterized by discontinuous segmental inflammation involving the ileum while sparing the proximal small intestine and colon. The histopathologic features of SAMP1/Fc ileitis include transmural inflammation, crypt abscesses, and epithelial changes including loss of villi, crypt elongation, and crypt branching. SAMP1/Fc mice were originally distinguished from the strain from which they were derived (SAMP1/Yit) by spontaneous onset of ileum inflammation by 10 weeks of age at nearly 100% penetrance and the emergence of a perianal fistulizing disease not reported in the parental strain. Other distinguishing characteristics of the SAMP1/Fc substrain include incidence of skin lesions inversely correlated with the occurrence of intestinal inflammation, chronic ileitis with prominent muscular hypertrophy and focal collagen deposition in inflamed segments, activation of mesenteric lymph node lymphocytes coincident with ileitis progression, and high interferon-γ production preceding ileitis onset. Like other spontaneous models of inflammatory bowel disease, SAMP1/Fc mice maintained at higher health status/germ-free conditions may exhibit attenuated ileitis onset and severity compared to SAMP1/Fc mice maintained in lower health status/specific pathogen-free vivaria.

Development
Siblings from a litter of AKR/J inbred mice (from The Jackson Laboratory; Stock No. 000648) were inadvertently outcrossed to an unknown mouse, and then bred together for more than 20 generations to generate a series of senescence prone strains. One of these senescence accelerated mice (SAM) strains, SAMP1, was identified with shortened life span and early signs of senescence (spontaneous amyloidosis, alopecia, and osteoporosis) by Dr. T Takeda (Kyoto University, Kyoto, Japan). SAMP1 mice were then sent to Dr. S Matsumoto (Yakult Central Institute for Microbiological Research, Tokyo, Japan), where mice showing spontaneous skin ulceration were selectively mated together. During a transfer from a conventional to specific pathogen free vivaria, mice lost the early senescence (amyloidosis) and developed a transmural, discontinuous ileitis and mild hepatic inflammation without necrosis. After more than 20 generations of inbreeding, the resulting SAMP1/Yit mice were established. In 1996, SAMP1/Yit mice were then sent to Dr. Fabio Cominelli (University of Virginia School of Medicine, Charlottesville Virginia, USA) where they were bred together for more than 20 generations. The resulting SAMP1/YitFcs mice (originally called SAMP1/YitFc or SAMP1/Fc) retained the high prevalence of spontaneous ileitis but also show unique characteristics compared with the original Japanese strain; incidence of skin lesions inversely correlated with the occurrence of intestinal inflammation, established ileitis as early as 10 weeks of age, chronic ileitis with prominent muscular hypertrophy and focal collagen deposition in inflamed segments, activation of mesenteric lymph node lymphocytes coincident with ileitis progression, high interferon-γ production preceding ileitis onset, and emergence of perianal disease with ulceration and fistulae. SAMP1/YitFcs mice are H-2^k; reported to have the entire MHC region on chromosome 17 identical to that of AKR/J mice. In 2009, SAMP1/YitFcs mice were sent from the University of Virginia School of Medicine to The Jackson Laboratory and assigned the strain name SAMP1/YitFcsJ.

Control Information

	Control
	000648 AKR/J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Dermatology Research
Skin and Hair Texture Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
      Inflammatory bowel disease
Inflammation
      Inflammatory bowel disease

Internal/Organ Research
Gastrointestinal Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Currently there are no related genes or alleles for this strain.

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Barnes SL; Vidrich A; Wang ML; Wu GD; Cominelli F; Rivera-Nieves J; Bamias G; Cohn SM. 2007. Resistin-like molecule beta (RELMbeta/FIZZ2) is highly expressed in the ileum of SAMP1/YitFc mice and is associated with initiation of ileitis. J Immunol 179(10):7012-20. [PubMed: 17982092]  [MGI Ref ID J:149582]

Kosiewicz MM; Nast CC; Krishnan A; Rivera-Nieves J; Moskaluk CA; Matsumoto S; Kozaiwa K; Cominelli F. 2001. Th1-type responses mediate spontaneous ileitis in a novel murine model of Crohn's disease. J Clin Invest 107(6):695-702. [PubMed: 11254669]  [MGI Ref ID J:71168]

Kozaiwa K; Sugawara K; Smith MF Jr; Carl V; Yamschikov V; Belyea B; McEwen SB; Moskaluk CA; Pizarro TT; Cominelli F; McDuffie M. 2003. Identification of a quantitative trait locus for ileitis in a spontaneous mouse model of Crohn's disease: SAMP1/YitFc. Gastroenterology 125(2):477-90. [PubMed: 12891551]  [MGI Ref ID J:84661]

Matsumoto S; Okabe Y; Setoyama H; Takayama K; Ohtsuka J; Funahashi H; Imaoka A; Okada Y; Umesaki Y. 1998. Inflammatory bowel disease-like enteritis and caecitis in a senescence accelerated mouse P1/Yit strain. Gut 43(1):71-8. [PubMed: 9771408]  [MGI Ref ID J:49022]

Rivera-Nieves J; Bamias G; Vidrich A; Marini M; Pizarro TT; McDuffie MJ; Moskaluk CA; Cohn SM; Cominelli F. 2003. Emergence of perianal fistulizing disease in the SAMP1/YitFc mouse, a spontaneous model of chronic ileitis. Gastroenterology 124(4):972-82. [PubMed: 12671894]  [MGI Ref ID J:83399]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, SAMP1/YitFcJ inbred mice are bred together. These inbred mice have significant breeding difficulties. The donating investigator reports: --it is best to maintain the colony in trio-matings --approximately 33% of trio-matings are nonproductive --approximately 33% of trio-matings will only produce one litter --productive matings produce approximately 2 litters total with an average of 4.8 mice per wean --if females are not pregnant by 6 weeks of breeding, that female is unlikely to produce any litters --the donating investigator does not set up mating pens until the mice are at least eight weeks old: even with this delayed mating, litters may not be observed for a few months --seasonal breeding slumps starting in January may further diminish colony breeding success.
Mating System	Sibling x Sibling         (Female x Male)   24-NOV-09
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls

 

This strain is currently Awaiting Transfer from the Donor.

To register your interest in this strain go to the Strain Interest Form.

View All Strains Awaiting Transfer from the Donor and On Hold

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000648 AKR/J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Important Note

These SAMP1 mice have significant breeding difficulties. Please see the JAXMice Health & Husbandry section for the SAMP1 mice Colony Maintenance / Breeding & Husbandry description.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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