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| These Osr1tm1Jian (Odd1-LacZ) mice harbor a nuclear localized β-galactosidase "knock-in" allele that also abolishes endogenous Osr1 (odd-skipped related 1 (Drosophila)) gene function. These mice may be useful as a lacZ reporter for Osr1 expression or as a knockout model for studying developmental biology (heart morphogenesis and urogenital development). | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Rulang Jiang, University of Rochester Description
The Osr1tm1Jian (Odd1-LacZ) mutation abolishes endogenous gene function and expresses a β-galactosidase fusion protein (fused in-frame with the N-terminal 16 amino acid residues of the endogenous protein). Under control of the upstream promoter/enhancer elements, lacZ expression is directed to the same tissues as the wild-type gene (for example, β-galactosidase expression during embryogenesis is detected at E7.5 in the intermediate mesoderm, is expanded to the gut endoderm, lung bud mesenchyme and myocardial cells by E9.5, and is activated in developing branchial arches and limb buds by E10.5). Almost all (`95%) of homozygous mice die in utero between E11.5-E12.5 from circulation distress; exhibiting malformed atrial septum, dilated atria with hypoplastic venous valves, and blood backflow from the heart into systemic veins. Homozygotes also exhibit complete agenesis of adrenal glands, metanephric kidneys, gonads, and defects in pericardium formation. Heterozygotes are viable and fertile. These Odd1-mutant mice may be useful as a lacZ reporter for Osr1 expression or as a knockout model for studying developmental biology (heart morphogenesis and urogenital development).Development
A targeting vector was designed to replace part of exon 2 of the Osr1 locus with the initial 16 codons of the Osr1 open reading frame fused in-frame with a nuclear localized β-galactosidase (lacZ) and loxP-flanked neo cassette. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females to establish the colony. Mice were then backcrossed to C57BL/6J for approximately 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony. The Y chromosome may not have been fixed to the genetic background during the backcross.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Osr1tm1Jian/Osr1tm1Jian
either: (involves: 129S1/Sv * C57BL/6J) or (involves: 129S1/Sv * ICR)
- mortality/aging
- complete lethality throughout fetal growth and development
- approximately 5% survive to E15.5-17.5 (MGI Ref ID J:104942)
- partial embryonic lethality during organogenesis
- most mutants embryos die between 11.5 and 12.5 (MGI Ref ID J:104942)
- embryogenesis phenotype
- abnormal mesonephros morphology
- at E11.5, fewer mesonephric tubules are observed and the gonadal ridge is retarded (MGI Ref ID J:104942)
- abnormal Wolffian duct morphology
- absent vitelline blood vessels
- at E12, yolk sac lacks blood circulation (MGI Ref ID J:104942)
- gonadal ridge hypoplasia
- a hypoplastic gonadal ridge is noted at E11.5 (MGI Ref ID J:104942)
- homeostasis/metabolism phenotype
- hydrops fetalis
- embryos at late gestation show generalized edema (MGI Ref ID J:104942)
- cardiovascular system phenotype
- abnormal blood circulation
- abnormal fetal atrioventricular canal morphology
- at E15.5, atrioventricular junction is malformed (MGI Ref ID J:104942)
- abnormal interatrial septum morphology
- septum primum does not form (MGI Ref ID J:104942)
- common atrium
- at E15.5, embryos lack a primary atrial septum (MGI Ref ID J:104942)
- abnormal interventricular septum morphology
- at E15.5, ventricular septum is malformed (MGI Ref ID J:104942)
- abnormal pericardium morphology
- at E15.5, mesothelium fails to form complete parietal pericardium (MGI Ref ID J:104942)
- abnormal vein morphology
- at E11.5, embryos have hypoplastic venous valves (MGI Ref ID J:104942)
- absent vitelline blood vessels
- at E12, yolk sac lacks blood circulation (MGI Ref ID J:104942)
- dilated heart atrium
- renal/urinary system phenotype
- abnormal kidney development
- at E10.5 to E11.5, mutant embryos fail to initiate metanephric kidney formation (MGI Ref ID J:104942)
- absent ureteric bud
- at E10.5 to E11.5, ureteric bud formation is absent (MGI Ref ID J:104942)
- increased kidney apoptosis
- at E10.5, massive cell death is noted in the nephrogenic mesenchyme (MGI Ref ID J:104942)
- endocrine/exocrine gland phenotype
- absent adrenal gland (MGI Ref ID J:104942)
- agonadal
- at E15.5-E17.5, all surviving mutants lack gonads (MGI Ref ID J:104942)
- reproductive system phenotype
- agonadal
- at E15.5-E17.5, all surviving mutants lack gonads (MGI Ref ID J:104942)
- respiratory system phenotype
- abnormal lung morphology
- at E15.5, lung tissue is observed as a compressed mass (MGI Ref ID J:104942)
- cellular phenotype
- increased kidney apoptosis
- at E10.5, massive cell death is noted in the nephrogenic mesenchyme (MGI Ref ID J:104942)
Osr1tm1Jian/Osr1tm1Jian
involves: 129S1/Sv
- embryogenesis phenotype
- abnormal mesonephros morphology
- abnormal Wolffian duct morphology
- a decreased diameter of the nephric duct is already seen at E8.5 (MGI Ref ID J:117734)
- at E9.5, the nephric duct is thinner and often interrupted (MGI Ref ID J:117734)
- at E10.5, the nephric duct fails to migrate toward the cloaca and metanephric mesenchyme, unlike in wild-type embryos (MGI Ref ID J:117734)
- renal/urinary system phenotype
- abnormal mesonephric mesenchyme morphology
- at E9.5 (but not at E8.5), significantly increased levels of apoptosis are detected in the mesenchyme surrounding anterior mesonephric tubules relative to control embryos (MGI Ref ID J:117734)
- abnormal metanephric mesenchyme morphology
- no metanephric mesenchyme condensation is observed at E11.5, unlike in wild-type controls (MGI Ref ID J:117734)
- absent metanephric mesenchyme
- at E11.5, the metanephric mesenchyme fails to form (MGI Ref ID J:117734)
- increased metanephric mesenchyme apoptosis
- at E10.5, increased levels of apoptosis are detected in the metanephric mesenchyme relative to heterozygous controls (MGI Ref ID J:117734)
- however, the levels of apoptosis in metanephric mesenchyme precursor cells are similarly low in E9.5 mutant and control embryos, indicating that apoptosis occurs after the initial metanephric mesenchyme gene expression defects (MGI Ref ID J:117734)
- absent metanephros
- at E10.5, several gene products required for metanephric kidney formation are not expressed in the metanephric mesenchyme region (MGI Ref ID J:117734)
- absent ureteric bud
- at E11.5, ureteric bud formation is completely absent (MGI Ref ID J:117734)
- cellular phenotype
- increased metanephric mesenchyme apoptosis
- at E10.5, increased levels of apoptosis are detected in the metanephric mesenchyme relative to heterozygous controls (MGI Ref ID J:117734)
- however, the levels of apoptosis in metanephric mesenchyme precursor cells are similarly low in E9.5 mutant and control embryos, indicating that apoptosis occurs after the initial metanephric mesenchyme gene expression defects (MGI Ref ID J:117734)
This mouse can be used to support research in many areas including:
Cardiovascular Research
Heart Abnormalities
Developmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects
adrenal cortex
gonads
heart
heart: vasculature
kidney
kidneys, gonads, heart
multiple
urogenital
vasculature
Mesodermal Defects
Internal/Organ Research
Adrenal Cortex Defects
Adrenal Medulla Defects
Heart Abnormalities
Kidney Defects
Research Tools
lacZ Expression
Cardiovascular Research
Developmental Biology Research
transplantation marker for embryonic and adult tissue
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: multiple
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Internal/Organ Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Osr1tm1Jian | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Rulang Jiang | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Odd1-; Odd1tm1Jian; Odd1tm1RJ; | ||
| Mutation Made By | Rulang Jiang, University of Rochester | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | CJ7 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Site of Expression | LacZ expression mimics that of the wild-type targeted gene. During embryogenesis is detected at E7.5 in the intermediate mesoderm, is expanded to the gut endoderm, lung bud mesenchyme and myocardial cells by E9.5, and is activated in developing branchial arches and limb buds by E10.5. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | A targeting construct was designed to insert a lacZ-loxP-neo-loxP cassette into exon 2. The cassette replaces 335 bp of the coding region. The expected protein would contain a functional beta-gal fused to the N-terminal 16 amino acids. X-gal staining detected lacZ activity corresponding to expected activity of the endogenous protein. [MGI Ref ID J:104942] | ||
| Gene Symbol and Name | Osr1, odd-skipped related 1 (Drosophila) | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | ODD; Odd1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Osr1tm1Jian SEP PCR, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wang Q; Lan Y; Cho ES; Maltby KM; Jiang R. 2005. Odd-skipped related 1 (Odd 1) is an essential regulator of heart and urogenital development. Dev Biol 288(2):582-94. [PubMed: 16223478] [MGI Ref ID J:104942]
Additional References
Osr1tm1Jian relatedGao Y; Lan Y; Liu H; Jiang R. 2011. The zinc finger transcription factors Osr1 and Osr2 control synovial joint formation. Dev Biol 352(1):83-91. [PubMed: 21262216] [MGI Ref ID J:171478]
James RG; Kamei CN; Wang Q; Jiang R; Schultheiss TM. 2006. Odd-skipped related 1 is required for development of the metanephric kidney and regulates formation and differentiation of kidney precursor cells. Development 133(15):2995-3004. [PubMed: 16790474] [MGI Ref ID J:117734]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred to wildtype siblings or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes die in utero.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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|
|
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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