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| These Osr2-IresCre (or Osr2IresCre) mice harbor an IRES-Cre inserted into the 3' UTR of Osr2 (odd-skipped related 2 (Drosophila)) locus. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence primarily in developing palate mesenchyme and metanephric mesenchyme-derived glomeruli tissues in the offspring. These mice may be useful for generating conditional mutations for studying developmental biology (palate and kidney development). | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Rulang Jiang, University of Rochester Description
Mice homozygous for the Osr2-IresCre (or Osr2IresCre) allele are viable and fertile, with an IRES-Cre bicistronic expression cassette inserted into the 3' UTR of the targeted locus. As such, cre expression is directed by the endogenous promoter/enhancer regions primarily to developing palate mesenchyme and metanephric mesenchyme-derived glomeruli tissues (but not other epithelial and mesenchymal tissues in the developing metanephric kidney). Some ectopic cre activity is reported (particularly in the central nervous system). When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence in these tissues in the offspring. These Osr2-IresCre mice may be useful for generating conditional mutations for studying developmental biology (palate and kidney development).View cre expression characterization.
Development
A targeting vector was designed to insert an IRES-Cre bicistronic expression cassette and frt-flanked neo cassette into the 3' UTR of exon 4 of the targeted locus. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females to establish the colony. Mice heterozygous for this Osr2-IresCre (or Osr2IresCre) allele were bred together to generate homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Facebase: tools
006084 B6.129P2(Cg)-Foxg1tm1(cre)Skm/J 003771 B6.Cg-Tg(Nes-cre)1Kln/J 005584 B6.Cg-Tg(Prrx1-cre)1Cjt/J 006774 FVB-Tg(Col2a1-cre/ERT)KA3Smac/J 012719 STOCK Tgfb3tm1(cre)Vk/J 004782 STOCK Tg(KRT14-cre)1Amc/J 005107 STOCK Tg(KRT14-cre/ERT)20Efu/J 003829 STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J View Facebase: tools (8 strains)
009386 B6.129S1-Osr2tm1Jian/J 010986 B6;129S-Osr2tm1Pzg/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Osr2tm2(cre)Jian/Osr2+
involves: 129S1/Sv * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- mice do not display any obvious defects (MGI Ref ID J:128736)
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Osr2tm2(cre)Jian | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Rulang Jiang | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Osr2-IresCre; Osr2-cre; Osr2IresCre; | ||
| Mutation Made By | Rulang Jiang, University of Rochester | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | CJ7 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Site of Expression | Expression of cre is detected in the palate and kidney during development. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Osr2 | ||
| Molecular Note | An IRES-cre cassette and an frt-flanked neo were inserted downstream of the stop codon. Expression of cre is detected in the palate and kidney during development. [MGI Ref ID J:128736] | ||
| Gene Symbol and Name | Osr2, odd-skipped related 2 | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | 5430409I15Rik; Osr2A; Osr2B; RIKEN cDNA 5430409I15 gene; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Osr2tm2(cre)Jian SEP PCR, Separated PCR
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Lan Y; Wang Q; Ovitt CE; Jiang R. 2007. A unique mouse strain expressing Cre recombinase for tissue-specific analysis of gene function in palate and kidney development. Genesis 45(10):618-24. [PubMed: 17941042] [MGI Ref ID J:128736]
Additional References
Osr2tm2(cre)Jian relatedBaek JA; Lan Y; Liu H; Maltby KM; Mishina Y; Jiang R. 2011. Bmpr1a signaling plays critical roles in palatal shelf growth and palatal bone formation. Dev Biol 350(2):520-31. [PubMed: 21185278] [MGI Ref ID J:170583]
Chen J; Lan Y; Baek JA; Gao Y; Jiang R. 2009. Wnt/beta-catenin signaling plays an essential role in activation of odontogenic mesenchyme during early tooth development. Dev Biol 334(1):174-85. [PubMed: 19631205] [MGI Ref ID J:153554]
Gao Y; Lan Y; Liu H; Jiang R. 2011. The zinc finger transcription factors Osr1 and Osr2 control synovial joint formation. Dev Biol 352(1):83-91. [PubMed: 21262216] [MGI Ref ID J:171478]
He F; Popkie AP; Xiong W; Li L; Wang Y; Phiel CJ; Chen Y. 2010. Gsk3beta is required in the epithelium for palatal elevation in mice. Dev Dyn 239(12):3235-46. [PubMed: 20981831] [MGI Ref ID J:166721]
Lan Y; Jiang R. 2009. Sonic hedgehog signaling regulates reciprocal epithelial-mesenchymal interactions controlling palatal outgrowth. Development 136(8):1387-96. [PubMed: 19304890] [MGI Ref ID J:147277]
Li J; Huang X; Xu X; Mayo J; Bringas P Jr; Jiang R; Wang S; Chai Y. 2011. SMAD4-mediated WNT signaling controls the fate of cranial neural crest cells during tooth morphogenesis. Development 138(10):1977-89. [PubMed: 21490069] [MGI Ref ID J:171428]
Xiong W; He F; Morikawa Y; Yu X; Zhang Z; Lan Y; Jiang R; Cserjesi P; Chen Y. 2009. Hand2 is required in the epithelium for palatogenesis in mice. Dev Biol 330(1):131-41. [PubMed: 19341725] [MGI Ref ID J:149232]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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|
|
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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