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| These mice express a codon-improved Cre recombinase (iCre) gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. These mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in brain tissues. | ||||||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Enhancer Trap; Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 30-SEP-09 Species laboratory mouse Generation N11+ (29-SEP-09)
Generation DefinitionsDonating Investigator Ronald Davis, The Scripps Research Institute (TSRI) Description
Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of a codon-improved Cre recombinase (iCre) gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. The donating investigator reports Cre recombinase activity in brain tissues (but may not have assessed expression in other tissues). When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.Development
An enhancer trap transgenic approach was used to generate these mice. The TK-bgi-icre-bGHpA transgene was designed with a thymidine kinase minimal promoter, a beta-globin intron, an optimized variant of Cre recombinase (iCre; improved with mammalian codon usage, removed putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), and a bovine growth hormone polyA signal. This transgene was injected into C57BL/6J embryos. Founder line 1470 was established on a C57BL/6J genetic background. Transgenic mice were subsequently bred for multiple generations with C57BL/6J mice and/or with Gt(ROSA)26Sortm1Sor mutant mice (backcrossed to C57BL/6 for at least 10 generations) prior to arrival at The Jackson Laboratory. Upon arrival, transgenic mice were selectively bred to C57BL/6J inbred mice (Stock No. 000664) to remove the Gt(ROSA)26Sortm1Sor mutation.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
NIH Neuroscience Blueprint cre strains from Baylor
009575 B6(129S4)-Et(cre/ERT2)119Rdav/J 009580 B6(129S4)-Et(cre/ERT2)1382Rdav/J 009581 B6(129S4)-Et(cre/ERT2)1642Rdav/J 009582 B6(129S4)-Et(cre/ERT2)1645Rdav/J 009583 B6(129S4)-Et(cre/ERT2)1957Rdav/J 009584 B6(129S4)-Et(cre/ERT2)2007Rdav/J 009585 B6(129S4)-Et(cre/ERT2)2047Rdav/J 009574 B6(129S4)-Et(cre/ERT2)21Rdav/J 009576 B6(129S4)-Et(cre/ERT2)278Rdav/J 009577 B6(129S4)-Et(cre/ERT2)296Rdav/J 009578 B6(129S4)-Et(cre/ERT2)398Rdav/J 009573 B6(129S4)-Et(cre/ERT2)4Rdav/J 009579 B6(129S4)-Et(cre/ERT2)837Rdav/J 009587 B6(129S4)-Et(icre)1402Rdav/J 009589 B6(129S4)-Et(icre)1555Rdav/J 009586 B6(129S4)-Et(icre)754Rdav/J View NIH Neuroscience Blueprint cre strains from Baylor (16 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Et(icre)1470Rdav | ||
|---|---|---|---|
| Allele Name | enhancer trap 1407, Ron Davis | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Strain of Origin | C57BL/6J | ||
| Gene Symbol and Name | Et(icre)1470Rdav, enhancer trap 1470, Ron Davis | ||
| Chromosome | UN | ||
| Driver Note | enhancer trap | ||
| Molecular Note | An enhancer trap transgenic approach was used to generate these mice. The TK-bgi-icre-bGHpA transgene was designed with the thymidine kinase minimal (TK) promoter minimal promoter driving a codon-improved cre recombinase sequence (icre). A beta globin intron separates the promoter from the cre gene and the bovine growth hormone polyadenylation signal follows the cre sequence. This transgene was injected into C57BL/6J embryos. Founder line 1470 was established on a C57BL/6J genetic background. [MGI Ref ID J:150856] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Standard PCR
Generic iCre, Melt Curve Analysis
Generic iCre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Davis R. 2009. Large-scale enhancer trap project to generate transgenic cre alleles by Ron Davis at Baylor University MGI Direct Data Submission :. [MGI Ref ID J:150856]
NIH Neuroscience Blueprint Cre Driver Network, URL: http://www.credrivermice.org/database. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data Submission :. [MGI Ref ID J:151755]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (noncarrier) siblings, or to C57BL/6J inbred mice (Stock No. 000664). Mating System Noncarrier x Hemizygote (Female x Male) 30-SEP-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Et(icre)1470Rdav
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Et(icre)1470Rdav x Noncarrier $297.85 Noncarrier x Hemizygous for Et(icre)1470Rdav Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Et(icre)1470Rdav
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Et(icre)1470Rdav x Noncarrier $387.30 Noncarrier x Hemizygous for Et(icre)1470Rdav Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
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Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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