Strain Name:

STOCK Adam17tm1.2Bbl/J

Stock Number:

009597

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These floxed mice harbor loxP sites flanking exon 2 of the TNF-α converting enzyme (TACE, also referred to as ADAM17 [a disintegrin and metallopeptidase domain 17]) locus. As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TACE, these Taceflox mice may be useful in generating conditional mutations for studying TNF-sheddase function and TNF-related autoimmune diseases.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHomozygote x Homozygote         (Female x Male)   02-NOV-10
Specieslaboratory mouse
GenerationF?+F11 (11-DEC-13)
Generation Definitions
 
Donating Investigator Carl P Blobel,   Hospital for Special Surgery-Weill Med

Description
Mice homozygous for this Taceflox allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed sequences deleted in the cre-expressing tissue producing a null allele. As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TNF-α converting enzyme (TACE or Adam17 [a disintegrin and metallopeptidase domain 17]), these Taceflox mutant mice may be useful in generating conditional mutations for studying TNF-sheddase function, TNF-related autoimmune diseases.

Development
A targeting vector was designed to insert a loxP site and frt-flanked PGK-neo cassette upstream of exon 2, and a second loxP site downstream of exon 2 of the targeted gene. The donating investigator reports that the construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 females to establish the colony. The mutant mice (C57BL/6;129P2/OlaHsd genetic background) were then bred to mice expressing Flp recombinase (B6;SJL genetic background; see Stock No. 003800) to remove the frt-flanked neo cassette. The resulting Taceflox mice (with loxP site and single frt site remaining upstream of exon 2, and a second loxP site downstream of exon 2) were maintained on this mixed genetic background for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   100492 B6129PF1/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Adam17
014138   C57BL/6J-Adam17m1Btlr/Mmjax
View Strains carrying other alleles of Adam17     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Inflammatory Skin and Bowel Disease, Neonatal; NISBD   (ADAM17)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Adam17tm1.2Bbl/Adam17tm1.2Bbl

        involves: 129P2/OlaHsd * C57BL/6
  • normal phenotype
  • no abnormal phenotype detected
    • mice are viable and fertile   (MGI Ref ID J:139846)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Growth Factors/Receptors/Cytokines

Cell Biology Research
Protein Processing

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      genes regulating susceptibility to infectious disease and endotoxin
Growth Factors/Receptors/Cytokines

Research Tools
Cancer Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Immunology, Inflammation and Autoimmunity Research
      genes regulating susceptibility to infectious disease and endotoxin

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Adam17tm1.2Bbl
Allele Name targeted mutation 1.2, Carl P Blobel
Allele Type Targeted (Floxed/Frt)
Mutation Made By Carl Blobel,   Hospital for Special Surgery-Weill Med
Strain of Origin129P2/OlaHsd
Gene Symbol and Name Adam17, a disintegrin and metallopeptidase domain 17
Chromosome 12
Gene Common Name(s) ADAM18; CD156B; CSVP; NISBD; TACE; Tace; tumor necrosis factor-alpha converting enzyme;
Molecular Note A targeting vector was designed to insert a loxP site and frt-flanked PGK-neo cassette upstream of exon 2, and a second loxP site downstream of exon 2 of the targeted gene. The resulting mutant mice were then bred to mice expressing Flp recombinase to remove the frt-flanked neo cassette. [MGI Ref ID J:139846]

Genotyping

Genotyping Information

Genotyping Protocols

Adam17tm1.2Bblalternate1, Standard PCR
Adam17tm1.2Bbl, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Horiuchi K; Kimura T; Miyamoto T; Miyamoto K; Akiyama H; Takaishi H; Morioka H; Nakamura T; Okada Y; Blobel CP; Toyama Y. 2009. Conditional inactivation of TACE by a Sox9 promoter leads to osteoporosis and increased granulopoiesis via dysregulation of IL-17 and G-CSF. J Immunol 182(4):2093-101. [PubMed: 19201862]  [MGI Ref ID J:144795]

Horiuchi K; Kimura T; Miyamoto T; Takaishi H; Okada Y; Toyama Y; Blobel CP. 2007. Cutting edge: TNF-alpha-converting enzyme (TACE/ADAM17) inactivation in mouse myeloid cells prevents lethality from endotoxin shock. J Immunol 179(5):2686-9. [PubMed: 17709479]  [MGI Ref ID J:139846]

Additional References

Adam17tm1.2Bbl related

Hall KC; Hill D; Otero M; Plumb DA; Froemel D; Dragomir CL; Maretzky T; Boskey A; Crawford HC; Selleri L; Goldring MB; Blobel CP. 2013. ADAM17 controls endochondral ossification by regulating terminal differentiation of chondrocytes. Mol Cell Biol 33(16):3077-90. [PubMed: 23732913]  [MGI Ref ID J:204569]

La Marca R; Cerri F; Horiuchi K; Bachi A; Feltri ML; Wrabetz L; Blobel CP; Quattrini A; Salzer JL; Taveggia C. 2011. TACE (ADAM17) inhibits Schwann cell myelination. Nat Neurosci 14(7):857-65. [PubMed: 21666671]  [MGI Ref ID J:174007]

Long C; Wang Y; Herrera AH; Horiuchi K; Walcheck B. 2010. In vivo role of leukocyte ADAM17 in the inflammatory and host responses during E. coli-mediated peritonitis. J Leukoc Biol 87(6):1097-101. [PubMed: 20154226]  [MGI Ref ID J:162272]

Maretzky T; Evers A; Zhou W; Swendeman SL; Wong PM; Rafii S; Reiss K; Blobel CP. 2011. Migration of growth factor-stimulated epithelial and endothelial cells depends on EGFR transactivation by ADAM17. Nat Commun 2:229. [PubMed: 21407195]  [MGI Ref ID J:205661]

Murthy A; Defamie V; Smookler DS; Di Grappa MA; Horiuchi K; Federici M; Sibilia M; Blobel CP; Khokha R. 2010. Ectodomain shedding of EGFR ligands and TNFR1 dictates hepatocyte apoptosis during fulminant hepatitis in mice. J Clin Invest 120(8):2731-44. [PubMed: 20628198]  [MGI Ref ID J:163773]

Nagao K; Kobayashi T; Ohyama M; Akiyama H; Horiuchi K; Amagai M. 2012. Brief report: requirement of TACE/ADAM17 for hair follicle bulge niche establishment. Stem Cells 30(8):1781-5. [PubMed: 22696231]  [MGI Ref ID J:197586]

Park JA; Sharif AS; Shiomi T; Kobzik L; Kasahara DI; Tschumperlin DJ; Voynow J; Drazen JM. 2013. Human neutrophil elastase-mediated goblet cell metaplasia is attenuated in TACE-deficient mice. Am J Physiol Lung Cell Mol Physiol 304(10):L701-7. [PubMed: 23564510]  [MGI Ref ID J:196680]

Xu H; Zhu J; Smith S; Foldi J; Zhao B; Chung AY; Outtz H; Kitajewski J; Shi C; Weber S; Saftig P; Li Y; Ozato K; Blobel CP; Ivashkiv LB; Hu X. 2012. Notch-RBP-J signaling regulates the transcription factor IRF8 to promote inflammatory macrophage polarization. Nat Immunol 13(7):642-50. [PubMed: 22610140]  [MGI Ref ID J:187656]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   02-NOV-10
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Adam17tm1.2Bbl  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Adam17tm1.2Bbl x Homozygous for Adam17tm1.2Bbl  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Adam17tm1.2Bbl  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Adam17tm1.2Bbl x Homozygous for Adam17tm1.2Bbl  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   100492 B6129PF1/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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