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| Mice hemizygous for the BAC LRRK2 transgene "overexpress" wildtype human leucine-rich repeat kinase 2 (LRRK2) gene directed by its endogenous promoter/enhancer regions on the BAC transgene. These BAC LRRK2 mice (also called WT-OX mice) are a control strain for the BAC LRRK2R1441G Parkinson's disease strain (Stock No. 009604). | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 04-NOV-09 Species laboratory mouse Generation N?+N3F5 (18-OCT-12)
Generation DefinitionsDonating Investigator Chenjian Li, Mount Sinai School of Medicine Description
Mice hemizygous for the BAC LRRK2 transgene are viable and fertile, with expression of a wild-type human leucine-rich repeat kinase 2 (LRRK2) gene directed by its endogenous promoter/enhancer regions on the BAC transgene. These BAC LRRK2 mice (also called WT-OX mice) "overexpress" the wildtype human LRRK2 protein in cortex, cerebellum, striatum and ventral midbrain at an approximately five- to ten-fold greater level than endogenous mouse Lrrk2, and are a control strain for the BAC LRRK2R1441G Parkinson's disease strain (Stock No. 009604). Contrary to the hypokinetic motor deficit of the BAC LRRK2R1441G Parkinson's disease mice, WT-OX mice exhibit slightly increased motor activities compared to nontransgenic wild-type controls.Development
A human bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 188 kb BAC containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene with 29 kb upstream of the LRRK2 start codon and 42 kb downstream of the LRRK2 stop codon. Bioinformatics analysis predicted that no other genes are present in the BAC. This BAC was microinjected into fertilized FVB/NTac zygotes. Founder BAC LRRK2 mice (also called WT-OX mice) were established and then subsequently maintained by breeding transgenic mice with FVB/N inbred mice for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Parkinson's Disease Models
View Parkinson's Disease Models (109 strains)
013583 B6.Cg-Tg(tetO-LRRK2)C7874Cai/J 012445 B6.FVB-Tg(LRRK2)WT1Mjfa/J 012446 B6.FVB-Tg(LRRK2*G2019S)1Mjfa/J 016575 B6;C3-Tg(PDGFB-LRRK2*G2019S)340Djmo/J 016576 B6;C3-Tg(PDGFB-LRRK2*R1441C)574Djmo/J 013725 B6;SJL-Tg(LRRK2)66Mjff/J 009609 FVB/N-Tg(LRRK2*G2019S)1Cjli/J 009604 FVB/N-Tg(LRRK2*R1441G)135Cjli/J 012441 STOCK Tg(tetO-LRRK2*G2019S)E3Cai/J 012449 STOCK Tg(teto-LRRK2)C7874Cai/J
Additional Web Information
Visit the Parkinson's Disease Resource site for helpful information on Parkinson's and research resources.
Phenotype
Phenotype Information
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Parkinson Disease 8, Autosomal Dominant; PARK8 (LRRK2)
assigned by genotype
Tg(LRRK2)1Cjli/0
FVB-Tg(LRRK2)1Cjli
- growth/size phenotype
- *normal* growth/size phenotype
- mice develop normally and do not exhibit progressive motor behavioral deficits (MGI Ref ID J:149135)
Tg(LRRK2)1Cjli/0
FVB/N-Tg(LRRK2)1Cjli/J
- cellular phenotype
- abnormal autophagy
- chaperone-mediated autophagy is impaired in primary neuronal cultures as determined by the use of a photoactivatable fluorescent reporter to visualize movement from cytosol to lysosome (MGI Ref ID J:194153)
- nervous system phenotype
- alpha-synuclein inclusion body
- exogenous and endogenous alpha-synuclein is found associated to lysosomes and organized into irreversible oligomeric complexes in primary neuronal cultures (MGI Ref ID J:194153)
This mouse can be used to support research in many areas including:
Neurobiology Research
Ataxia (Movement) Defects
Neurodegeneration
Parkinson's Disease
LRRK2 strains
Research Tools
Neurobiology Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(LRRK2)1Cjli | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Chenjian Li | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | BAC WT LRRK2; TG-WT-OX; WT-OX; | ||
| Mutation Made By | Chenjian Li, Mount Sinai School of Medicine | ||
| Strain of Origin | FVB/NTac | ||
| Expressed Gene | LRRK2, leucine-rich repeat kinase 2, human | ||
| Promoter | LRRK2, leucine-rich repeat kinase 2, human | ||
| Molecular Note | The human bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 188 kb BAC containing the entire human LRRK2 (leucine-rich repeat kinase 2) gene with 29 kb upstream of the LRRK2 start codon and 42 kb downstream of the LRRK2 stop codon. Bioinformatics analysis predicted that no other genes are present in the BAC. This BAC was microinjected into fertilized FVB/NTac zygotes. [MGI Ref ID J:149135] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(LRRK2), QPCR
LRRK2*R1441G, Sanger sequencing
Tg(LRRK2) , Melt Curve Analysis
Tg(LRRK2) , Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Li Y; Liu W; Oo TF; Wang L; Tang Y; Jackson-Lewis V; Zhou C; Geghman K; Bogdanov M; Przedborski S; Beal MF; Burke RE; Li C. 2009. Mutant LRRK2(R1441G) BAC transgenic mice recapitulate cardinal features of Parkinson's disease. Nat Neurosci 12(7):826-8. [PubMed: 19503083] [MGI Ref ID J:149135]
Additional References
Tg(LRRK2)1Cjli relatedOrenstein SJ; Kuo SH; Tasset I; Arias E; Koga H; Fernandez-Carasa I; Cortes E; Honig LS; Dauer W; Consiglio A; Raya A; Sulzer D; Cuervo AM. 2013. Interplay of LRRK2 with chaperone-mediated autophagy. Nat Neurosci 16(4):394-406. [PubMed: 23455607] [MGI Ref ID J:194153]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to FVB/NJ inbred mice (Stock No. 001800). Mating System Noncarrier x Hemizygote (Female x Male) 04-NOV-09
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(LRRK2)1Cjli
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(LRRK2)1Cjli x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(LRRK2)1Cjli Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(LRRK2)1Cjli
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(LRRK2)1Cjli x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(LRRK2)1Cjli Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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