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| These TgNL2.6 mice harbor have expression of a hemagglutinin-tagged rat neuroligin 2 (Nlgn2 or NL2) gene driven by the murine Thy1.2 expression cassette. TgNL2.6 mice manifest jumping behavior, limb clasping, anxiety, and impaired social interactions and may be useful in neurobiological studies of neuroligin adhesion molecules in excitatory/inhibitory synapse maturation and function, as well as altered behavior associated with neurodevelopmental disorders (such as autism and Rett syndrome). | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N7pN1
Generation DefinitionsDonating Investigator Rochelle M Hines, University of British Columbia Description
Mice hemizygous for the TgNL2 transgene are viable and fertile, but hemizygous females are poor mothers. The TgNL2 transgene encodes a hemagglutinin-tagged rat neuroligin 2 (Nlgn2 or NL2) gene driven by the murine Thy1.2 expression cassette. HA-NL2 transcript and protein is expressed throughout the neuroaxis in neuronal cells (high levels in cortex and limbic structures such as amygdala and hippocampus) and is predominantly localized to inhibitory synaptic contacts. TgNL2.6 mice have moderate to high levels of HA-NL2 expression (approximately 1.6-fold greater than wildtype NL2). This overexpression leads to reduced lifespan and body weight, and induces aberrant synapse maturation and altered neuronal excitability that lead to behavioral deficits. Specifically, TgNL2.6 mice manifest disorders reminiscent of autism and/or Rett syndrome; jumping stereotypies, limb clasping, anxiety, and impaired social interactions. Transgenic mice also exhibit Straub tail, transient episodes of kyphosis, and enhanced incidence of spike-wave discharges. These TgNL2.6 mice may be useful in neurobiological studies of neuroligin adhesion molecules in excitatory/inhibitory synapse maturation and function, as well as altered behavior associated with neurodevelopmental disorders (such as autism and Rett syndrome).Development
The TgNL2 transgene was designed with a modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns) upstream of a hemagglutinin-tagged rat neuroligin 2 (Nlgn2 or NL2) cDNA. This transgene was injected into (C57BL/6xCBA)F1 cells. Transgenic founders were bred with C57BL/6 and mice from founder line 6 (TgNL2.6) were found to have an approximately 1.6-fold increase in NL2 expression and moderate phenotype severity. The donating investigator reports that because TgNL2.6 females are poor mothers, this strain was then maintained by breeding C57BL/6 females with TgNL2.6 males for at least nine generations prior to arrival at The Jackson Laboratory. Upon arrival, TgNL2.6 mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Nlgn2
008139 B6;129-Nlgn2tm1Bros/J View Strains carrying other alleles of Nlgn2 (1 strain)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Thy1-Nlgn2)6Hnes/0
involves: C57BL/6 * CBA
- mortality/aging
- premature death
- mice show reduced life span compared to controls or Tg(Thy1-Nlgn1)6Hnes mice (MGI Ref ID J:137376)
- growth/size phenotype
- slow postnatal weight gain
- mice display lower body weight at postnatal time points compared to wild-type controls (MGI Ref ID J:137376)
- behavior/neurological phenotype
- *normal* behavior/neurological phenotype
- abnormal response to novelty
- in a three chambered apparatus, mutants show no preference for the chamber containing the novel object/mouse, unlike wild-type animals which show a strong preference for the chamber with the novelty (MGI Ref ID J:137376)
- abnormal social investigation
- total interaction time of male mutants with a novel juvenile animal of a different strain is significantly reduced compared to wild-type controls; number of interactions with the novel animal is not significantly different but average duration is much lower (MGI Ref ID J:137376)
- hyperactivity
- animals have elevated basal activity levels (MGI Ref ID J:137376)
- increased anxiety-related response
- in light/dark tests, mice show increased time spent in dark compartment compared to controls, but number of transitions from light to dark compartments is similar to control animals (MGI Ref ID J:137376)
- in elevated plus maze paradigm, mutants spent larger percentage of time in open arms and in number of entries into open arms relative to wild-type (MGI Ref ID J:137376)
- increased thigmotaxis
- mice show increase in both average and cumulative distance from arena/cage border compared to wild-type (MGI Ref ID J:137376)
- increased startle reflex
- mice have an enhanced startle reflex (MGI Ref ID J:137376)
- increased stereotypic behavior
- spontaneous jumping stereotypies are observed in corners of the open-field apparatus where jumping is typically preceded by rearing against the wall and is characterized by repeated jumping vertically on hindlimbs and balancing on rigid tail (MGI Ref ID J:137376)
- mice display about 10 episodes over a 5 minute period (MGI Ref ID J:137376)
- elevated incidence of other types of stereotypic behaviors such as digging and grooming are not observed in the open field compared to wild-type (MGI Ref ID J:137376)
- increased vertical activity
- in the open field, mice display increased rearing frequency indicative of elevated anxiety (MGI Ref ID J:137376)
- limb grasping
- limb clasping is observed (MGI Ref ID J:137376)
- straub tail
- animals display Straub tail (MGI Ref ID J:137376)
- nervous system phenotype
- *normal* nervous system phenotype
- somatosensory, visual, auditory, and olfactory systems are intact (MGI Ref ID J:137376)
- abnormal brain morphology
- mice show changes in synapse morphology in frontal cortex; synaptic component morphology and synaptic densities are altered (MGI Ref ID J:137376)
- number of vesicles at synapses is increased compared to wild-type; area of symmetric presynaptic compartments and contact length of symmetric synapses are increased (MGI Ref ID J:137376)
- significant increase in asymmetric presynaptic compartment area is observed (MGI Ref ID J:137376)
- average length of postsynaptic densities is unchanged relative to controls (MGI Ref ID J:137376)
- abnormal brain wave pattern
- most mutants tested display brief bilateral bursting activity characterized by spike-wave discharge of 6-8 Hz, while wild-type do not; episodes do not correlate with specific behaviors and are detected during different sleep and wake states (MGI Ref ID J:137376)
- signs of seizure are not observed with these anomalous discharge episodes (MGI Ref ID J:137376)
- abnormal miniature inhibitory postsynaptic currents
- skeleton phenotype
- kyphosis
- animals display transient episodes of kyphosis (MGI Ref ID J:137376)
This mouse can be used to support research in many areas including:
Cell Biology Research
Defects in Cell Adhesion Molecules
Developmental Biology Research
Defects in Cell Adhesion Molecules
Mouse/Human Gene Homologs
Rett syndrome
Neurobiology Research
Behavioral and Learning Defects
high anxiety
Neurodevelopmental Defects
Autism
Rett's syndrome
Neurotransmitter Receptor and Synaptic Vesicle Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-Nlgn2)6Hnes | ||
|---|---|---|---|
| Allele Name | transgene insertion 6, Rochelle Hines | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TgNL2.6; | ||
| Mutation Made By | Rochelle Hines, University of British Columbia | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Expressed Gene | Nlgn2, neuroligin 2, mouse, laboratory | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | The TgNL2 transgene was designed with a modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns) upstream of a hemagglutinin-tagged rat neuroligin 2 (Nlgn2 or NL2) cDNA. Transgenic founders were bred with C57BL/6 and mice from founder line 6 (TgNL2.6) were found to have an approximately 1.6-fold increase in NL2 expression and moderate phenotype severity, [MGI Ref ID J:137376] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-Nlgn1)6Hnes STD PCR, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hines RM; Wu L; Hines DJ; Steenland H; Mansour S; Dahlhaus R; Singaraja RR; Cao X; Sammler E; Hormuzdi SG; Zhuo M; El-Husseini A. 2008. Synaptic imbalance, stereotypies, and impaired social interactions in mice with altered neuroligin 2 expression. J Neurosci 28(24):6055-67. [PubMed: 18550748] [MGI Ref ID J:137376]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous males may be bred to wildtype (noncarrier) siblings or to C57BL/6J inbred mice (Stock No. 000664). The donating investigator reports that hemizygous females are poor mothers. If transgenic females are to be used for breeding, the donating investigator recommends mating multiple transgenic females together with the male to improve pup survival.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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