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| These A930038C07Rik-Tg4-Cre mice express Cre recombinase under the control of the A930038C07Rik (RIKEN cDNA A930038C07 gene [also called epidermacan]) promoter/enhancer regions, and may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, striatum, and cerebellum). | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;C3-Tg(A930038C07Rik-cre)4Aibs/J (Changed: 04-AUG-09 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 06-NOV-09 Species laboratory mouse Generation N5+ (05-NOV-09) Donating Investigator Theresa Zwingman, Allen Institute for Brain Science (AIBS) Donating Investigator Ed Lein, Allen Institute for Brain Science (AIBS) Description
Hemizygous A930038C07Rik-Tg4-Cre mice are viable and fertile, with cre expression directed to cortex, striatum, and cerebellum by the A930038C07Rik promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These A930038C07Rik-Tg4-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, striatum, and cerebellum).For characterization information, see images at the Allen Institute for Brain Science website (for example, A930038C07Rik-Tg4-Cre images (1), A930038C07Rik-Tg4-Cre images (2), and/or A930038C07Rik-Tg4-Cre images (3)).
Development
The 196 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-476E22, containing the entire A930038C07Rik locus (and other genes), was modified by targeting a Cre recombinase coding sequence (the CreERT2 sequence modified back to encode the normal Cre recombinase gene), SV40 polyA signal, and frt-flanked neomycin cassette to the ATG start site of the A930038C07Rik locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into embryos from B6C3 mice ((C57BL/6 x C3H)F1 x C57BL/6). Females from founder line A930038C07Rik-Tg4-Cre were bred to C57BL/6J males to generate the colony. Mice were subsequently backcrossed to C57BL/6J mice for one additional generation prior to arrival at The Jackson Laboratory. Upon arrival, A930038C07Rik-Tg4-Cre mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish this colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100010 B6C3F1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Allen Institute for Brain Science cre strains
009613 B6.Cg-Tg(Scnn1a-cre)3Aibs/J 009614 B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J 008844 B6;C3-Tg(Ctgf-cre)2Aibs/J 008839 B6;C3-Tg(Cyp39a1-cre)1Aibs/J 009117 B6;C3-Tg(Cyp39a1-cre)7Aibs/J 008848 B6;C3-Tg(Mybpc1-cre)2Aibs/J 009111 B6;C3-Tg(Scnn1a-cre)1Aibs/J 009112 B6;C3-Tg(Scnn1a-cre)2Aibs/J 009103 B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J 009615 STOCK Tg(Cartpt-cre)1Aibs/J View Allen Institute for Brain Science cre strains (10 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Tg(A930038C07Rik-cre)4Aibs/0
involves: C3H * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:146821)
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(A930038C07Rik-cre)4Aibs | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Ed Lein | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | A930038C07Rik-Tg4-Cre; | ||
| Mutation Made By | Ed Lein, Allen Institute for Brain Science (AIBS) | ||
| Strain of Origin | (C57BL/6 x C3H)F1 X C57BL/6 | ||
| Site of Expression | Hemizygous A930038C07Rik-Tg4-Cre mice are viable and fertile, with cre expression directed to cortex, striatum, and cerebellum by the A930038C07Rik promoter/enhancer regions within the BAC transgene. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | A930038C07Rik, RIKEN cDNA A930038C07 gene, mouse, laboratory | ||
| Driver Note | A930038C07Rik | ||
| Molecular Note | The 196 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-476E22, containing the entire A930038C07Rik locus (and other genes), was modified by targeting a Cre recombinase coding sequence (the CreERT2 sequence modified back to encode the normalCre recombinase gene), SV40 polyA signal, and frt-flanked neomycin cassette to the ATG start site of the A930038C07Rik locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into embryos from B6C3 mice ((C57BL/6 x C3H)F1 x C57BL/6). Founder line 4 was established. [MGI Ref ID J:146821] | ||
| Gene Symbol and Name | Tg(A930038C07Rik-cre)4Aibs, transgene insertion 4, Ed Lein | ||
| Chromosome | UN | ||
| Gene Common Name(s) | A930038C07Rik-Tg4-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Allen Institute for Brain Science. 2009. Cre transgenes created at the Allen Insitute for Brain Science (AIBS) (http://transgenicmouse.alleninstitute.org/imageseries/list/1.html?gene term=) MGI Direct Data Submission :. [MGI Ref ID J:146821]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (noncarrier) siblings, or to C57BL/6J inbred mice (Stock No. 000664). Mating System Noncarrier x Hemizygote (Female x Male) 06-NOV-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100010 B6C3F1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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