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| Cre recombinase expression is seen during development and in the adult in pituitary somatotropes. Weaker expression is detected in lactotropes. Cre expression is detected in the forelimb skin of embryos aged embryonic day 16.5-17.5 with weaker expression in adult kidney, ovary, and testis. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful for studying developmentally critical gene function in the anterior pituitary. | |||||||||||||||
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 23-OCT-09 Species laboratory mouse Generation N5+ (22-OCT-09) Donating Investigator Sally Camper, University of Michigan Medical School Description
These transgenic mice express Cre recombinase under the control of the human Growth Hormone (GH1) Locus Control Region and the rat growth hormone 1, (Gh1) promoter. When crossed with a reporter strain, Cre recombinase expression is seen during development and in the adult in pituitary somatotropes. Weaker expression is detected in lactotropes. Cre expression is detected in the forelimb skin of embryos aged embryonic day 16.5-17.5, and weaker expression in adult kidney, ovary, and testis. Southern blot analysis reveals approximately 50 copies or the transgene segregated to one locus. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the anterior pituitary.Development
A transgenic construct containing sequence encoding Cre recombinase under the control of the human Growth Hormone (GH1) Locus Control Region and the rat growth hormone 1, (Gh1), promoter was introduced into (C57BL/6J x SJL)F1 donor eggs. Founder animals were bred to wild-type C57BL/6J. The mice were bred to a conditional (floxed) mutation strain on the 129 background and then bred to C57BL/6J for several generations to remove the floxed allele. There may be residual 129 genetic background contribution. The Donating Investigator has not attempted to make this strain homozygous.
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (162 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Reproductive Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Gh1-cre)1Sac | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Sally A Camper | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(Gh-cre)SAC1; | ||
| Mutation Made By | Sally Camper, University of Michigan Medical School | ||
| Strain of Origin | (C57BL/6J x SJL/J)F2 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Gh1, growth hormone 1, rat | ||
| Driver Note | Gh1 | ||
| Molecular Note | The human growth hormone locus control region (-16.2 to -14.6 kb relative to the GH1 promoter) was fused to 1.77 kb of rat Gh1 promoter to drive expression of a cre recombinase cassette in the pituitary gland. About 30-50 copies of the transgene construct inserted into the genome after injection into zygotes. When crossed with cre-reporter strains, cre expression was observed in the pituitary somatotropes, with limited expression in lactotropes, and no detectable expression in other pituitary cell types. Cre expression was also detected in the skin of embryonic forelimbs and trunk, and in adult testis, ovary, and kidney. [MGI Ref ID J:144723] | ||
| Gene Symbol and Name | Tg(Gh1-cre)1Sac, transgene insertion 1, Sally A Camper | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(Gh-cre)SAC1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Nasonkin IO; Potok MA; Camper SA. 2009. Cre-mediated recombination in pituitary somatotropes. Genesis 47(1):55-60. [PubMed: 19039787] [MGI Ref ID J:144723]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. Mating System Noncarrier x Hemizygote (Female x Male) 23-OCT-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 23-NOV-09
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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