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| These transgenic mice express Cre recombinase under the control of the bovine LHB, luteinizing hormone beta polypeptide promoter. Cre recombinase expression is seen in mature pituitary gonadotropes, specifically in cells that produce luteinizing hormone beta. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in the study of developmentally critical gene function in the pituitary. | |||||||||||||||
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Sally Camper, University of Michigan Medical School Description
These transgenic mice express Cre recombinase under the control of the bovine LHB, luteinizing hormone beta polypeptide promoter. Although the transgenic construct contains sequence encoding a fusion gene of EGFP and Cre recombinase, no EGFP fluorescence is detected. When crossed with a reporter strain, Cre recombinase expression is seen in mature pituitary gonadotropes, specifically in cells that produce luteinizing hormone beta. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the pituitary.Development
A transgenic construct containing sequence encoding an EGFP and Cre recombinase fusion gene under the control of the bovine LHB, luteinizing hormone beta polypeptide, promoter was introduced into (C57BL/6J x SJL)F1 donor eggs. Founder animals were bred to wildtype C57BL/6J. The mice were bred to a conditional (floxed) mutation strain on the 129 background and then bred to C57BL/6J for several generations to remove the floxed allele. There may be residual 129 genetic background contribution. The Donating Investigator has not attempted to make this strain homozygous.
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (162 strains)
006619 STOCK Tg(Cga-LHB/CGB)94Jhn/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Reproductive Biology Research
Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Lhb-cre)1Sac | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Sally A Camper | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Strain of Origin | (C57BL/6J x SJL)F1 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | LHB, luteinizing hormone beta polypeptide, bovine | ||
| Driver Note | Lhb | ||
| Molecular Note | The bovine gonadotrope-specific enhancer was used to drive expression of an EGFP/cre fusion gene in the pituitary cells that produce luteinizing hormone beta. No GFP signal could be detected in the one mouse line created. [MGI Ref ID J:142726] | ||
| Gene Symbol and Name | Tg(Lhb-cre)1Sac, transgene insertion 1, Sally A Camper | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Charles MA; Mortensen AH; Potok MA; Camper SA. 2008. Pitx2 deletion in pituitary gonadotropes is compatible with gonadal development, puberty, and fertility. Genesis 46(10):507-14. [PubMed: 18802953] [MGI Ref ID J:142726]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
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Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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