Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse

Description
The recessive misty mutation causes a mild dilution of coat color and on certain backgrounds a white tail tip often accompanied by a belly spot. Melanocytes from m/m mice have a highly dendritic shape, show deficient proliferation in culture and have much more melanin content. Fewer melanoblasts are found in primary cultures from m/m mice than from wildtype controls. Between two and five weeks of age, m/m mice are smaller than controls. At 35 days of age they are shorter, weigh 15% less on average, and have less inguinal adipose mass than controls. Misty homozygotes completely lack brown fat. Although platelet count, seratonin content and ATP content are normal, there is increased bleed time and reduced platelet AD levels in m/m homozygotes. (Woolley, 1941 and 1945; Truett et al., 1998; Sviderskaya et al., 1998.)

Development
The coat color mutation, misty (m), arose spontaneously in a DBA/J colony at The Jackson Laboratory in 1941. The single male was identified by a light color, white tail tip and white belly spot. Misty was introgressed into C57BLKS-Lepr^db to create the repulsion stock, BKS.Cg-Dock7^m +/+ Lepr^db/J. The two alleles were used to identify genotypes in the diabetes colony prior to the use of PCR genotyping. Both mutations then were backcrossed to the C57BL/6 background. In 2008, misty was bred out of the B6.Cg-Dock7^m +/+ Lepr^db/J colony.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Dock7^m allele

002048	B6 x C57BLKS-Dock7m Leprdb Myo15sh2-J/J
000699	B6.Cg-Dock7m Leprdb/+ +/J
000027	B6.D-Tyrp1b Dock7m/J
000642	BKS.Cg-Dock7m +/+ Leprdb/J
000700	BKS.Cg-Dock7m Leprdb/+ +/J
001049	BKS.Cg-mea2J Dock7m/+ +/J
001192	BKS.Cg-meaJ Leprdb +/+ + Dock7m/J
000707	CBA.Cg-Dock7m Leprdb/+ +/J

View Strains carrying   Dock7^m     (8 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Storage Pool Platelet Disease

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Dock7^m/Dock7^m

        B6.D(Cg)-Dock7^m

• adipose tissue phenotype
• abnormal brown adipose tissue amount
  • appears to be completely absent in neonatal mice   (MGI Ref ID J:45425)
• decreased white adipose tissue amount
  • mice have 21% less inguinal adipose mass   (MGI Ref ID J:48831)
• growth/size phenotype
• decreased body length
  • mutants are 8% shorter   (MGI Ref ID J:48831)
• decreased body weight
  • mutants weigh 15% less   (MGI Ref ID J:48831)
• hematopoietic system phenotype
• decreased platelet ADP level
  • platelet ADP levels are low   (MGI Ref ID J:45425)
  • however, the platelet count, and platelet serotonin and ATP levels are normal   (MGI Ref ID J:45425)
• homeostasis/metabolism phenotype
• increased bleeding time   (MGI Ref ID J:45425)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Dock7^m/Dock7^m

        DBA/J

• pigmentation phenotype
• diluted coat color
  • coat color is not as diluted as that of homozygous dilute (Myo5a^d) or homozygous leaden (Mlph^ln) mice   (MGI Ref ID J:311)
  • individual hairs have more cortical pigment than found in homozygous dilute or homozygous leaden mice   (MGI Ref ID J:311)
• variable body spotting
  • white tail tip with or without a white belly spot   (MGI Ref ID J:311)
• integument phenotype
• diluted coat color
  • coat color is not as diluted as that of homozygous dilute (Myo5a^d) or homozygous leaden (Mlph^ln) mice   (MGI Ref ID J:311)
  • individual hairs have more cortical pigment than found in homozygous dilute or homozygous leaden mice   (MGI Ref ID J:311)
• variable body spotting
  • white tail tip with or without a white belly spot   (MGI Ref ID J:311)

Dock7^m/Dock7^m

        BKS.Cg-Dock7^m +/+ Lepr^db/BomTac

• behavior/neurological phenotype
• *normal* behavior/neurological phenotype
  • homozygous mutant and wild type control (C57BL/6J) mice exhibit similar locomotor activity; response in the tail-suspension test for depressive behavior; learning as measured by habituation of object exploration; working memory as measured using the Y maze; anxiety-like behavior in a light-dark transfer test; and preference for social familiarity/novelty   (MGI Ref ID J:146458)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Dermatology Research
Color and White Spotting Defects

Dock7^m related

Dermatology Research
Color and White Spotting Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Dock7m
Allele Name		misty
Allele Type		Spontaneous
Common Name(s)		m;
Strain of Origin		DBA/J
Gene Symbol and Name		Dock7, dedicator of cytokinesis 7
Chromosome		4
Gene Common Name(s)		3110056M06Rik; Gm430; LOC242555; RIKEN cDNA 3110056M06 gene; ZIR2; gene model 430, (NCBI); m; mKIAA1771; misty;
Molecular Note		Crosses between mice homozygous for misty and for moonlight, which mapped to overlapping critical regions on Chr 4, demonstrated failure of the two mutations to complement one another. Once moonlight had been identified as a mutation of Dock7 (Dock7mnlt), sequence analysis of this gene from misty mice revealed a retrotransposon LTR insertion following nucleotide 2045 (numbering from the A of the transcription initiation codon) that interrupts exon 18 and shifts the reading frame after codon 682 so that ten incorrect amino acids are incorporated into the protein before its premature termination. [MGI Ref ID J:146458]

Genotyping

Genotyping Information

Genotyping Protocols

Dock7^m, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Woolley GW. 1941. "Misty," a new coat color dilution in the mouse, Mus musculus. Am Naturalist 75:507-508.  [MGI Ref ID J:12159]

Additional References

Dock7^m related

Akasaka Y; Tsunoda M; Ogata T; Ide T; Murakami K. 2010. Direct evidence for leptin-induced lipid oxidation independent of long-form leptin receptor. Biochim Biophys Acta 1801(10):1115-22. [PubMed: 20601111]  [MGI Ref ID J:165456]

Alipio Z; Liao W; Roemer EJ; Waner M; Fink LM; Ward DC; Ma Y. 2010. Reversal of hyperglycemia in diabetic mouse models using induced-pluripotent stem (iPS)-derived pancreatic beta-like cells. Proc Natl Acad Sci U S A 107(30):13426-31. [PubMed: 20616080]  [MGI Ref ID J:162403]

Banks AS; Kon N; Knight C; Matsumoto M; Gutierrez-Juarez R; Rossetti L; Gu W; Accili D. 2008. SirT1 gain of function increases energy efficiency and prevents diabetes in mice. Cell Metab 8(4):333-41. [PubMed: 18840364]  [MGI Ref ID J:143422]

Bermudez DM; Herdrich BJ; Xu J; Lind R; Beason DP; Mitchell ME; Soslowsky LJ; Liechty KW. 2011. Impaired biomechanical properties of diabetic skin implications in pathogenesis of diabetic wound complications. Am J Pathol 178(5):2215-23. [PubMed: 21514435]  [MGI Ref ID J:171585]

Blasius AL; Brandl K; Crozat K; Xia Y; Khovananth K; Krebs P; Smart NG; Zampolli A; Ruggeri ZM; Beutler BA. 2009. Mice with mutations of Dock7 have generalized hypopigmentation and white-spotting but show normal neurological function. Proc Natl Acad Sci U S A 106(8):2706-11. [PubMed: 19202056]  [MGI Ref ID J:146458]

Campo GM; Avenoso A; Micali A; Nastasi G; Squadrito F; Altavilla D; Bitto A; Polito F; Rinaldi MG; Calatroni A; D'Ascola A; Campo S. 2010. High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice. Biochim Biophys Acta 1802(11):1118-30. [PubMed: 20713153]  [MGI Ref ID J:170003]

Eller P; Eller K; Kirsch AH; Patsch JJ; Wolf AM; Tagwerker A; Stanzl U; Kaindl R; Kahlenberg V; Mayer G; Patsch JR; Rosenkranz AR. 2011. A murine model of phosphate nephropathy. Am J Pathol 178(5):1999-2006. [PubMed: 21514417]  [MGI Ref ID J:171593]

Flach RJ; Qin H; Zhang L; Bennett AM. 2011. Loss of mitogen-activated protein kinase phosphatase-1 protects from hepatic steatosis by repression of cell death-inducing DNA fragmentation factor A (DFFA)-like effector C (CIDEC)/fat-specific protein 27. J Biol Chem 286(25):22195-202. [PubMed: 21521693]  [MGI Ref ID J:174820]

Iwakura H; Akamizu T; Ariyasu H; Irako T; Hosoda K; Nakao K; Kangawa K. 2007. Effects of ghrelin administration on decreased growth hormone status in obese animals. Am J Physiol Endocrinol Metab 293(3):E819-25. [PubMed: 17595213]  [MGI Ref ID J:125421]

Jung YJ; Choi HJ; Lee JE; Lee AS; Kang KP; Lee S; Park SK; Park TS; Jin HY; Lee SY; Kim DH; Kim W. 2012. The effects of designed angiopoietin-1 variant on lipid droplet diameter, vascular endothelial cell density and metabolic parameters in diabetic db/db mice. Biochem Biophys Res Commun 420(3):498-504. [PubMed: 22430141]  [MGI Ref ID J:183490]

Kanda Y; Shimoda M; Hamamoto S; Tawaramoto K; Kawasaki F; Hashiramoto M; Nakashima K; Matsuki M; Kaku K. 2010. Molecular mechanism by which pioglitazone preserves pancreatic beta-cells in obese diabetic mice: evidence for acute and chronic actions as a PPARgamma agonist. Am J Physiol Endocrinol Metab 298(2):E278-86. [PubMed: 19920213]  [MGI Ref ID J:170065]

Mazagova M; Buikema H; Landheer SW; Vavrinec P; Buiten Av; Henning RH; Deelman LE. 2013. Growth differentiation factor 15 impairs aortic contractile and relaxing function through altered caveolar signaling of the endothelium. Am J Physiol Heart Circ Physiol 304(5):H709-18. [PubMed: 23262134]  [MGI Ref ID J:194601]

Moore KJ; Swing DA; Copeland NG; Jenkins NA. 1990. Interaction of the murine dilute suppressor gene (dsu) with fourteen coat color mutations [published erratum appears in Genetics 1990 Sep;126(1):285] Genetics 125(2):421-30. [PubMed: 2379821]  [MGI Ref ID J:29467]

Nuno DW; Harrod JS; Lamping KG. 2009. Sex-dependent differences in Rho activation contribute to contractile dysfunction in type 2 diabetic mice. Am J Physiol Heart Circ Physiol 297(4):H1469-77. [PubMed: 19666843]  [MGI Ref ID J:154218]

Olmsted-Davis E; Gannon FH; Ozen M; Ittmann MM; Gugala Z; Hipp JA; Moran KM; Fouletier-Dilling CM; Schumara-Martin S; Lindsey RW; Heggeness MH; Brenner MK; Davis AR. 2007. Hypoxic adipocytes pattern early heterotopic bone formation. Am J Pathol 170(2):620-32. [PubMed: 17255330]  [MGI Ref ID J:117897]

Park CW; Kim HW; Lim JH; Yoo KD; Chung S; Shin SJ; Chung HW; Lee SJ; Chae CB; Kim YS; Chang YS. 2009. Vascular endothelial growth factor inhibition by dRK6 causes endothelial apoptosis, fibrosis, and inflammation in the heart via the Akt/eNOS axis in db/db mice. Diabetes 58(11):2666-76. [PubMed: 19675133]  [MGI Ref ID J:154385]

San Martin A; Du P; Dikalova A; Lassegue B; Aleman M; Gongora MC; Brown K; Joseph G; Harrison DG; Taylor WR; Jo H; Griendling KK. 2007. Reactive oxygen species-selective regulation of aortic inflammatory gene expression in Type 2 diabetes. Am J Physiol Heart Circ Physiol 292(5):H2073-82. [PubMed: 17237245]  [MGI Ref ID J:125944]

Schatteman GC; Awad O; Nau E; Wang C; Jiao C; Tomanek RJ; Dunnwald M. 2010. Lin- cells mediate tissue repair by regulating MCP-1/CCL-2. Am J Pathol 177(4):2002-10. [PubMed: 20813969]  [MGI Ref ID J:165434]

Shimoda M; Kanda Y; Hamamoto S; Tawaramoto K; Hashiramoto M; Matsuki M; Kaku K. 2011. The human glucagon-like peptide-1 analogue liraglutide preserves pancreatic beta cells via regulation of cell kinetics and suppression of oxidative and endoplasmic reticulum stress in a mouse model of diabetes. Diabetologia :. [PubMed: 21340625]  [MGI Ref ID J:169587]

Silvers WK. 1979. The Coat Colors of Mice; A Model for Mammalian Gene Action and Interaction. In: The Coat Colors of Mice. Springer-Verlag, New York.  [MGI Ref ID J:78801]

Streicher C; Zeitz U; Andrukhova O; Rupprecht A; Pohl E; Larsson TE; Windisch W; Lanske B; Erben RG. 2012. Long-term Fgf23 deficiency does not influence aging, glucose homeostasis, or fat metabolism in mice with a nonfunctioning vitamin D receptor. Endocrinology 153(4):1795-805. [PubMed: 22294750]  [MGI Ref ID J:183813]

Sviderskaya EV; Novak EK; Swank RT; Bennett DC. 1998. The murine misty mutation: phenotypic effects on melanocytes, platelets and brown fat. Genetics 148(1):381-90. [PubMed: 9475748]  [MGI Ref ID J:45425]

Swank RT; Novak EK; McGarry MP; Rusiniak ME; Feng L. 1998. Mouse models of Hermansky Pudlak syndrome: a review. Pigment Cell Res 11(2):60-80. [PubMed: 9585243]  [MGI Ref ID J:88018]

Syeda MM; Jing X; Mirza RH; Yu H; Sellers RS; Chi Y. 2012. Prostaglandin transporter modulates wound healing in diabetes by regulating prostaglandin-induced angiogenesis. Am J Pathol 181(1):334-46. [PubMed: 22609345]  [MGI Ref ID J:185546]

Truett GE; Tempelman RJ; Walker JA; Wilson JK. 1998. Misty (m) affects growth traits. Am J Physiol 275(1 Pt 2):R29-32. [PubMed: 9688956]  [MGI Ref ID J:48831]

Velmurugan GV; White C. 2012. Calcium homeostasis in vascular smooth muscle cells is altered in type 2 diabetes by Bcl-2 protein modulation of InsP3R calcium release channels. Am J Physiol Heart Circ Physiol 302(1):H124-34. [PubMed: 22037186]  [MGI Ref ID J:181575]

Wendt TM; Tanji N; Guo J; Kislinger TR; Qu W; Lu Y; Bucciarelli LG; Rong LL; Moser B; Markowitz GS; Stein G; Bierhaus A; Liliensiek B; Arnold B; Nawroth PP; Stern DM; D'Agati VD; Schmidt AM. 2003. RAGE Drives the Development of Glomerulosclerosis and Implicates Podocyte Activation in the Pathogenesis of Diabetic Nephropathy. Am J Pathol 162(4):1123-37. [PubMed: 12651605]  [MGI Ref ID J:82491]

Woolley GW. 1945. Misty dilution in the mouse J Hered 36:269-70.  [MGI Ref ID J:311]

Yaguchi H; Togawa K; Moritani M; Itakura M. 2005. Identification of candidate genes in the type 2 diabetes modifier locus using expression QTL. Genomics 85(5):591-9. [PubMed: 15820311]  [MGI Ref ID J:97535]

Yamamoto N; Matsubara T; Sobue K; Tanida M; Kasahara R; Naruse K; Taniura H; Sato T; Suzuki K. 2012. Brain insulin resistance accelerates Abeta fibrillogenesis by inducing GM1 ganglioside clustering in the presynaptic membranes. J Neurochem 121(4):619-28. [PubMed: 22260232]  [MGI Ref ID J:184309]

Yang J; Park Y; Zhang H; Gao X; Wilson E; Zimmer W; Abbott L; Zhang C. 2009. Role of MCP-1 in tumor necrosis factor-alpha-induced endothelial dysfunction in type 2 diabetic mice. Am J Physiol Heart Circ Physiol 297(4):H1208-16. [PubMed: 19666844]  [MGI Ref ID J:154217]

Yang Y; Gurung B; Wu T; Wang H; Stoffers DA; Hua X. 2010. Reversal of preexisting hyperglycemia in diabetic mice by acute deletion of the Men1 gene. Proc Natl Acad Sci U S A 107(47):20358-63. [PubMed: 21059956]  [MGI Ref ID J:166586]

Zhong X; Chung AC; Chen HY; Dong Y; Meng XM; Li R; Yang W; Hou FF; Lan HY. 2013. miR-21 is a key therapeutic target for renal injury in a mouse model of type 2 diabetes. Diabetologia 56(3):663-74. [PubMed: 23292313]  [MGI Ref ID J:194766]

Zimmermann C; Cederroth CR; Bourgoin L; Foti M; Nef S. 2012. Prevention of diabetes in db/db mice by dietary soy is independent of isoflavone levels. Endocrinology 153(11):5200-11. [PubMed: 22962258]  [MGI Ref ID J:191798]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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