Strain Name:

B6.129-Ppargc1atm2.1Brsp/J

Stock Number:

009666

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Availability:

Repository- Live

These Ppargc1a (peroxisome proliferative activated receptor, gamma, coactivator 1 alpha) conditional (floxed) mutant mice may be useful in generating conditional mutations for studying hepatic heme biosynthesis and porphyrias as well as neuromuscular junction physiology and muscular dystrophy.

Description

Strain Information

Former Names B6.129-Ppargc1atm2Brsp/J    (Changed: 12-AUG-14 )
Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   08-JAN-10
Specieslaboratory mouse
GenerationN8+F14 (10-DEC-13)
Generation Definitions
 
Donating Investigator Bruce Spiegelman,   Dana-Farber Cancer Institute

Description
These mice possess loxP sites on either side of exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-5 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase specifically in the liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatic heme biosynthesis and porphyrias.

When bred to a strain expressing Cre recombinase specifically in skeletal muscle, this mutant mouse strain may be useful in studies of neuromuscular junction physiology and muscular dystrophy.

Development
A loxP site flanked targeting vector containing neomycin resistance and thymidine kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 5 of the targeted gene, and another loxP site was inserted upstream of exon 3. This construct was electroporated into ?Sv129? derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then crossed to transgenic mice expressing Cre recombinase under the control of the adenovirus EIIa promoter (B6.FVB-Tg(EIIa-cre)C5379Lmgd/J) to remove the neo selection cassette. Mice that retained the loxP site flanked exons 3-5 were then bred to C57BL/6N mice for 7 generations. The EIIa-cre transgene has been removed. Upon arrival at The Jackson Laboratory the mice were crossed with C57BL/6NJ once to establish the colony.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Ppargc1a
008597   B6.129-Ppargc1atm1Brsp/J
009662   B6.129X1-Ppargc1atm1Dpk/J
008231   C57BL/6-Tg(Ckm-Ppargc1a)31Brsp/J
012387   FVB-Tg(tetO-Ppargc1a)1Dpk/J
View Strains carrying other alleles of Ppargc1a     (4 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cardiovascular Research
      Cre-lox System
Cell Biology Research
Cre-lox System
      loxP-flanked Sequences
Metabolism Research
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ppargc1atm2.1Brsp
Allele Name targeted mutation 2.1, Bruce M Spiegelman
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Pgc-1alphafl;
Strain of Origin129
Gene Symbol and Name Ppargc1a, peroxisome proliferative activated receptor, gamma, coactivator 1 alpha
Chromosome 5
Gene Common Name(s) A830037N07Rik; ENSMUSG00000079510; Gm11133; LEM6; PGC-1(alpha); PGC-1v; PGC1; PGC1A; PPAR Gamma Coactivator-1; PPAR gamma coactivator 1; PPARGC1; Pgc-1alpha; Pgc-1alphaa; Pgco1; RIKEN cDNA A830037N07 gene; predicted gene 11133; predicted gene, ENSMUSG00000079510;
Molecular Note Exons 3-5 were flanked by loxP sites. A neo with a third loxP site was located downstream of the floxed exons. Cre-mediated recombination removed the selection cassette. [MGI Ref ID J:208380]

Genotyping

Genotyping Information

Genotyping Protocols

Ppargc1atm2Brsp STD PCR, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lin J; Wu PH; Tarr PT; Lindenberg KS; St-Pierre J; Zhang CY; Mootha VK; Jager S; Vianna CR; Reznick RM; Cui L; Manieri M; Donovan MX; Wu Z; Cooper MP; Fan MC; Rohas LM; Zavacki AM; Cinti S; Shulman GI; Lowell BB; Krainc D; Spiegelman BM. 2004. Defects in adaptive energy metabolism with CNS-linked hyperactivity in PGC-1alpha null mice. Cell 119(1):121-35. [PubMed: 15454086]  [MGI Ref ID J:93896]

Additional References

Ppargc1atm2.1Brsp related

Sczelecki S; Besse-Patin A; Abboud A; Kleiner S; Laznik-Bogoslavski D; Wrann CD; Ruas JL; Haibe-Kains B; Estall JL. 2014. Loss of Pgc-1alpha expression in aging mouse muscle potentiates glucose intolerance and systemic inflammation. Am J Physiol Endocrinol Metab 306(2):E157-67. [PubMed: 24280126]  [MGI Ref ID J:208380]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   08-JAN-10
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHomozygous for Ppargc1atm2.1Brsp  
Price per Pair (US dollars $)Pair Genotype
$478.00Homozygous for Ppargc1atm2.1Brsp x Homozygous for Ppargc1atm2.1Brsp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHomozygous for Ppargc1atm2.1Brsp  
Price per Pair (US dollars $)Pair Genotype
$621.40Homozygous for Ppargc1atm2.1Brsp x Homozygous for Ppargc1atm2.1Brsp  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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