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| When crossed with a Cre strain, the Gt(ROSA)26Sor promoter in these mice drives expression of diptheria toxin, enabling selective ablation in a tissue/cell-specific manner. | |||||||||||||||
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Richard Locksley, Univ of California San Francisco-HHMI Description
When crossed with a Cre strain, the Gt(ROSA)26Sor promoter in these mice drives expression of diptheria toxin, enabling selective ablation in a tissue/cell-specific manner. Homozygotes are viable, fertile and do not display any gross physical or behavioral abnormalities.Development
A flox-stop-flox-diptheria toxin subunit alpha gene cassette was knocked into intron 1 of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for 10 generations by the donating laboratory.
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (64 strains)
Introduction to Cre-lox technology
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sortm1(DTA)Lky
involves: 129P2/OlaHsd
- cellular phenotype
- abnormal cell death (MGI Ref ID J:133382)
- T cells infected with a retroviral cre exhibit cell death without inducing cell death in uninfected cells
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
| Allele Symbol | Gt(ROSA)26Sortm1(DTA)Lky | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard M Locksley | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R-DTA; | ||
| Mutation Made By | Richard Locksley, Univ of California San Francisco-HHMI | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A floxed neo/STOP cassette and DTA (diphtheria toxin alpha chain) gene were inserted into the locus. Expression of DTA is only achieved following cre-mediated removal of the floxed neo/STOP cassette. [MGI Ref ID J:133382] | ||
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
Voehringer D; Liang HE; Locksley RM. 2008. Homeostasis and effector function of lymphopenia-induced 'memory-like' T cells in constitutively T cell-depleted mice. J Immunol 180(7):4742-53. [PubMed: 18354198] [MGI Ref ID J:133382]
Gt(ROSA)26Sortm1(DTA)Lky relatedKang SJ; Liang HE; Reizis B; Locksley RM. 2008. Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells. Immunity 29(5):819-33. [PubMed: 19006696] [MGI Ref ID J:142395]
Ohnmacht C; Pullner A; King SB; Drexler I; Meier S; Brocker T; Voehringer D. 2009. Constitutive ablation of dendritic cells breaks self-tolerance of CD4 T cells and results in spontaneous fatal autoimmunity. J Exp Med 206(3):549-59. [PubMed: 19237601] [MGI Ref ID J:146781]
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygotes or heterozygotes may be bred.
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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