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| When crossed with a Cre strain, the Gt(ROSA)26Sor promoter in these mice drives expression of diptheria toxin, enabling selective ablation in a tissue/cell-specific manner. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Richard Locksley, Univ of California San Francisco-HHMI Description
When crossed with a Cre strain, the Gt(ROSA)26Sor promoter in these mice drives expression of diptheria toxin, enabling selective ablation in a tissue/cell-specific manner. Homozygotes are viable, fertile and do not display any gross physical or behavioral abnormalities.Development
A flox-stop-flox-diptheria toxin subunit alpha gene cassette was knocked into intron 1 of the targeted gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. This line was backcrossed to C57BL/6 for 10 generations by the donating laboratory.
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (64 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm1(DTA)Lky/Gt(ROSA)26Sortm1(DTA)Lky
involves: 129P2/OlaHsd
- cellular phenotype
- abnormal cell death (MGI Ref ID J:133382)
- T cells infected with a retroviral cre exhibit cell death without inducing cell death in uninfected cells
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(DTA)Lky | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard M Locksley | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R-DTA; | ||
| Mutation Made By | Richard Locksley, Univ of California San Francisco-HHMI | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A floxed neo/STOP cassette and DTA (diphtheria toxin alpha chain) gene were inserted into the locus. Expression of DTA is only achieved following cre-mediated removal of the floxed neo/STOP cassette. [MGI Ref ID J:133382] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Voehringer D; Liang HE; Locksley RM. 2008. Homeostasis and effector function of lymphopenia-induced 'memory-like' T cells in constitutively T cell-depleted mice. J Immunol 180(7):4742-53. [PubMed: 18354198] [MGI Ref ID J:133382]
Additional References
Gt(ROSA)26Sortm1(DTA)Lky relatedKang SJ; Liang HE; Reizis B; Locksley RM. 2008. Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells. Immunity 29(5):819-33. [PubMed: 19006696] [MGI Ref ID J:142395]
Ohnmacht C; Pullner A; King SB; Drexler I; Meier S; Brocker T; Voehringer D. 2009. Constitutive ablation of dendritic cells breaks self-tolerance of CD4 T cells and results in spontaneous fatal autoimmunity. J Exp Med 206(3):549-59. [PubMed: 19237601] [MGI Ref ID J:146781]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygotes or heterozygotes may be bred.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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