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| Egln1 (EGL nine homolog 1 (C. elegans)) homozygous targeted mutation mice are embryonic lethal at approximately E12.5 due to placental and/or cardiac malformation. No mRNA or protein are detectable in mouse embryonic fibroblasts (MEFs) derived from homozyous mice. This strain may be useful in studies of development. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator William Kaelin, Dana Farber Cancer Institute Description
Homozygous targeted mutation mice are embryonic lethal at approximately E12.5 due to placental and/or cardiac malformation. No mRNA or protein are detectable in mouse embryonic fibroblasts (MEFs) derived from homozyous mice. This strain may be useful in studies of development.Development
An FRT-flanked neomycin resistance cassette was placed in intron 1 of the gene. LoxP sites were introduced just 5' of the cassette and in intron 3. The 129S6/SvEvTac-derived TC1 embryonic stem (ES) cell line was used to create the mutation. A cross with an FVB-actin-flipase strain excised the neomycin cassette and a subsequent FVB EIIA-Cre transgenic line cross removed exons 2 and 3 (which encode almost the entire catalytic domain). The line was backcrossed five times to C57BL/6 by the donating lab to remove the Cre transgene.
Related Strains
Strains carrying other alleles of Egln1
009672 STOCK Egln1tm1Kael/J View Strains carrying other alleles of Egln1 (1 strain)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Egln1tm1.1Kael/Egln1tm1.1Kael
involves: 129S6/SvEvTac * C57BL/6 * FVB/N
- mortality/aging
- complete lethality during fetal growth through weaning
- viable embryos are present at E12.5, but are not present in the offspring of heterozygous matings (MGI Ref ID J:132718)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects
heart
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Egln1tm1.1Kael | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, William G Kaelin | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | PHD2-; | ||
| Mutation Made By | Yoji Andrew Minamishima, Dana-Farber Cancer Institute (DFCI) | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC1/TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Egln1, EGL nine homolog 1 (C. elegans) | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | open reading frame 13; AI503754; C1orf12; ECYT3; HIF-PH2; HIFPH2; HPH-2; HPH2; Hif-p4h-2; ORF13; PHD-2; PHD2; SM-20; SM20; ZMYND6; expressed sequence AI503754; open reading frame 13; | ||
| Molecular Note | The floxed exon 2 and 3 of Egln1tm1Kael were removed via cre-mediated recombination by mating with Tg(EIIa-cre)C5379Lmgd mice. Egln1 mRNA and protein are undetectable in MEFs isolated from E12.5 embryos. [MGI Ref ID J:132718] | ||
References
References provided by MGI
Selected Reference(s)
Minamishima YA; Moslehi J; Bardeesy N; Cullen D; Bronson RT; Kaelin WG Jr. 2008. Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure. Blood 111(6):3236-44. [PubMed: 18096761] [MGI Ref ID J:132718]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes may be bred. Homozygotes are embryonic lethal.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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