Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129-Pygo2tm1Lan/J    (Changed: 24-JUN-10 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x Heterozygote         (Female x Male)   20-JAN-10 Species laboratory mouse   Donating Investigator Richard A. Lang,   Cincinnati Children's Hospital

Description
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in eye tissues results in microophthalmia and abnormal lens induction. This mutant mouse strain is useful in studies of lens development, but may also be useful in studies of other organs as well.

For example, when crossed to a strain with widespread Cre recombinase expression (see Stock No. 003465 and 006054), this mutant mouse strain may be useful in lens development.

When crossed to a strain expressing Cre recombinase in midbrain and spinal cord (see Stock No. 007807), this mutant mouse strain may be useful in studies of lens development.

Development
A targeting vector was designed to place a loxP site upstream of exon 3, and a frt-flanked Pgk-Neo cassette and loxP site donstream of exon 3 of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were used to generate chimeric males. Chimeric males may or may not have been bred with Swiss Black mice. At some point, the mice were bred to a FLP recombinase-expressing strain to remove the frt-flanked Pgk-Neo cassette. These mice were maintained by intercrossing prior to sending to The Jackson Laboratory. Agouti mice were received. Upon arrival, the mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.

Control Information

	Control
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Pygo2^tm1.1Ssp/Pygo2^tm1.1Lan Tg(Wnt1-cre)11Rth/?

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA   (conditional)

• vision/eye phenotype
• small lens
  • at E12.5, lens size is reduced comparable to Pygo2^tm1Lan/Pygo2^tm1.1Lan mice   (MGI Ref ID J:121416)

Pygo2^tm1.1Ssp/Pygo2^tm1.1Ssp Tg(CMV-cre)1Cgn/Y

        involves: 129S1/Sv * 129X1/SvJ * BALB/cJ   (conditional)

• mortality/aging
• partial neonatal lethality
  • most mice die neonatally   (MGI Ref ID J:121416)
• vision/eye phenotype
• abnormal optic cup morphology
  • optic cups are misshapen with abnormal retinal folds and excess mesenchyme cells   (MGI Ref ID J:121416)
  • however, retinal differentiation and retinal pigment epithelium are grossly normal   (MGI Ref ID J:121416)
• aphakia
  • in all day-of-birth mice lenses were small or absent   (MGI Ref ID J:121416)
• microphthalmia   (MGI Ref ID J:121416)
• small lens
  • in all day-of-birth mice lenses were small or absent   (MGI Ref ID J:121416)
• renal/urinary system phenotype
• abnormal kidney development
  • mild kidney development defects   (MGI Ref ID J:121416)
• growth/size phenotype
• decreased fetal size
  • mice are smaller late gestation   (MGI Ref ID J:121416)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Pygo2tm1.1Ssp
Allele Name		targeted mutation 1, S Steven Potter
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Pygo2flox; Pygo2tm1Lan;
Mutation Made By		Richard Lang,   Cincinnati Children's Hospital
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Pygo2, pygopus 2
Chromosome		3
Gene Common Name(s)		1190004M21Rik; RGD1308535; RIKEN cDNA 1190004M21 gene; mpygo2;
Molecular Note		LoxP sites were inserted to flank exon 3. [MGI Ref ID J:121416]

Genotyping

Genotyping Information

Genotyping Protocols

Pygo2^tm1Lan, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Song N; Schwab KR; Patterson LT; Yamaguchi T; Lin X; Potter SS; Lang RA. 2007. pygopus 2 has a crucial, Wnt pathway-independent function in lens induction. Development 134(10):1873-85. [PubMed: 17428831]  [MGI Ref ID J:121416]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony these mice are bred as homozygotes.
Mating System	Heterozygote x Heterozygote         (Female x Male)   20-JAN-10

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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