Strain Name:

STOCK Smtnl1tm1Tajh/J

Stock Number:

010503

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Cryopreserved - Ready for recovery

This targeted mutation of the smoothelin-like 1 (Smtnl1) gene displays an abnormal response to exercise adaptation in muscle fibers and may be useful in studies of muscle and vascular adaptation to exercise.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129S.129P2-Smtnl1tm1Tajh/J    (Changed: 02-FEB-10 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Timothy Haystead,   Duke University Medical Center

Description
Mice homozygous for this targeted mutation are viable, fertile and normal in size. No gene product is detected by Western blot analysis. Mutant mice exhibit better performance during endurance training and, males in particular, exhibit increased time to fatigue, distance run and vertical work in stress tests after endurance training. Vasorelaxation and contractile responses are improved in mutant mice and the ratio of type 2a muscle fibers is increased in sedentary mice, however, this ratio does not change following exercise. This mutant mouse strain may be useful in studies of muscle and vascular adaptation to exercise.

Development
A targeting vector was designed to replace exons 3-8 of the targeted gene with a neomycin resistance cassette. The construct was electroporated into 129P2/OlaHsd-derived E14TG2A embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6 blastocysts, and chimeric males were bred to 129S6/SvEvTac females. Heterozygous offspring were bred to 129S6/SvEvTac mice for approximately 11 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation prior to intercrossing to generate homozygotes.

A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 3 of 27 markers (on chromsomes 1, 13 and 15) that were not 129 allele-type. These data suggest genetic contamination (perhaps from C57BL/6 or other) prior to arrival at The Jackson Laboratory.

Control Information

  Control
   002448 129S1/SvImJ (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Smtnl1tm1Tajh/Smtnl1tm1Tajh

        129S6.129P2-Smtnl1tm1Tajh
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • despite altered vascular physiology, capillary contacts and fatigue time is normal   (MGI Ref ID J:136573)
    • abnormal blood vessel physiology
      • sedentary mice exhibit aorta adaptations similar to those observed in exercised wild-type mice   (MGI Ref ID J:136573)
      • decreased vasoconstriction
        • following treatment with phenylephrine, the aortic ring exhibits decreased contractile force compared to in similarly treated wild-type mice   (MGI Ref ID J:136573)
        • however, mice do not exhibit decreased sensitivity to phenylephrine and tracheal rings exhibit normal vasocontriction   (MGI Ref ID J:136573)
      • increased vasodilation
        • following exposure to achetylcholine, sedentary mice exhibit increased vascular relaxation of the aortic ring compared to sedentary wild-type mice that was similar to the relaxation seen in exercised wild-type mice   (MGI Ref ID J:136573)
        • however, mice exhibit normal tracheal ring vasodilation   (MGI Ref ID J:136573)
  • muscle phenotype
  • abnormal skeletal muscle fiber type ratio
    • sedentary mice exhibit an increase in the amount of type 2a fibers and a decrease in the amount of type 1b fibers that is similar to that observed in exercised wild-type mice   (MGI Ref ID J:136573)
    • further exercise does not increase the number of type 2a fibers as in wild-type mice   (MGI Ref ID J:136573)
    • however, skeletal muscle ultrastructure is normal   (MGI Ref ID J:136573)
  • decreased vasoconstriction
    • following treatment with phenylephrine, the aortic ring exhibits decreased contractile force compared to in similarly treated wild-type mice   (MGI Ref ID J:136573)
    • however, mice do not exhibit decreased sensitivity to phenylephrine and tracheal rings exhibit normal vasocontriction   (MGI Ref ID J:136573)
  • increased vasodilation
    • following exposure to achetylcholine, sedentary mice exhibit increased vascular relaxation of the aortic ring compared to sedentary wild-type mice that was similar to the relaxation seen in exercised wild-type mice   (MGI Ref ID J:136573)
    • however, mice exhibit normal tracheal ring vasodilation   (MGI Ref ID J:136573)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • despite altered vascular physiology, mice exhibit normal blood pressure and chemistries   (MGI Ref ID J:136573)
    • enhanced exercise endurance
      • male mice exhibit increased exercise endurance with increased time to fatigue, distance run and vertical work performed compared to wild-type mice   (MGI Ref ID J:136573)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects

Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Smtnl1tm1Tajh
Allele Name targeted mutation 1, Timothy A J Haystead
Allele Type Targeted (Null/Knockout)
Mutation Made By Timothy Haystead,   Duke University Medical Center
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Smtnl1, smoothelin-like 1
Chromosome 2
Gene Common Name(s) 1110030K22Rik; CHASM; RGD1311701; RIKEN cDNA 1110030K22 gene;
Molecular Note Exons 3 through 8, which encode amino acids 230-421, were replaced with a neo cassette. The absence of protein product was confirmed by western blot on skeletal muscle extracts. [MGI Ref ID J:136573]

Genotyping

Genotyping Information

Genotyping Protocols

Smtnl1tm1Tajh, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wooldridge AA; Fortner CN; Lontay B; Akimoto T; Neppl RL; Facemire C; Datto MB; Kwon A; McCook E; Li P; Wang S; Thresher RJ; Miller SE; Perriard JC; Gavin TP; Hickner RC; Coffman TM; Somlyo AV; Yan Z; Haystead TA. 2008. Deletion of the protein kinase A/protein kinase G target SMTNL1 promotes an exercise-adapted phenotype in vascular smooth muscle. J Biol Chem 283(17):11850-9. [PubMed: 18310078]  [MGI Ref ID J:136573]

Additional References

Smtnl1tm1Tajh related

Bodoor K; Lontay B; Safi R; Weitzel DH; Loiselle D; Wei Z; Lengyel S; McDonnell DP; Haystead TA. 2011. Smoothelin-like 1 protein is a bifunctional regulator of the progesterone receptor during pregnancy. J Biol Chem 286(36):31839-51. [PubMed: 21771785]  [MGI Ref ID J:176612]

Lontay B; Bodoor K; Weitzel DH; Loiselle D; Fortner C; Lengyel S; Zheng D; Devente J; Hickner R; Haystead TA. 2010. Smoothelin-like 1 protein regulates myosin phosphatase-targeting subunit 1 expression during sexual development and pregnancy. J Biol Chem 285(38):29357-66. [PubMed: 20634291]  [MGI Ref ID J:166394]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony heterozygous females are bred with homozygous males. The donating investigator reports a decreased litter size in homozygous matings.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   002448 129S1/SvImJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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