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| These Ly-49A transgenic mice express the mouse Klra1 (killer cell lectin-like receptor, subfamily A, member 1) gene under the control of a modified mouse CD4 antigen (Cd4) promoter (this promoter lacks the silencer element sequence suppressing expression in CD8 T cells). Transgene expression is detected during early T cell development and throughout CD4 and CD8 T cell differentiation and migration. This mutant mouse strain may be useful in studies of autoimmune and inflammatory diseases. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Mary Pauza, Southern Illinois University Description
These Ly-49A transgenic mice express the mouse Klra1 (killer cell lectin-like receptor, subfamily A, member 1) gene under the control of a modified mouse CD4 antigen (Cd4) promoter (this promoter lacks the silencer element sequence suppressing expression in CD8 T cells). Transgene expression is detected during early T cell development and throughout CD4 and CD8 T cell differentiation and migration. On the B10.HTG background, which lacks H2dd, cell surface transgene expression is increased when compared to transgenic mice on backgrounds carrying H2dd. The presence of H2dd ligand during T cell development impairs negative selection in the thymus and can result in increased apoptosis of thymocytes, fewer CD4 single positive cells, and autoreactive T cells with the development of a graft-versus-host-disease-like syndrome. This mutant mouse strain may be useful in studies of autoimmune and inflammatory diseases.Development
The Ly-49A transgenic construct containing the mouse Klra1 (killer cell lectin-like receptor, subfamily A, member 1) gene under the control of a modified mouse CD4 antigen (Cd4) promoter (this promoter lacks the silencer element sequence suppressing expression in CD8 T cells) was injected into fertilized C57BL/6 mouse eggs. Founder line 6295 was established. The mice were then crossed to B10.HTG mice for at least 10 generations. These mice carry the H2g allele.
Related Strains
Strains carrying other alleles of Cd4
002447 B10.129S2(B6)-Cd4tm1Litt/J 002663 B6.129S2-Cd4tm1Mak/J 002269 B6.129S6-Cd4tm1Knw/J 002664 B6;129S-Cd4tm1Mak Cd8atm1Mak/J 002268 B6;129S6-Cd4tm1Knw/J 012895 C57BL/6-Tg(Cd4-Il17rb)5Cdon/J 014639 C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J 014644 C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J 006483 CBy.129S2(B6)-Cd4tm1Mak/J 003090 NOD.129S6(B6)-Cd4tm1Knw/DvsJ 005328 NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J View Strains carrying other alleles of Cd4 (11 strains)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Cd4-Klra1)6295Dl/0
involves: C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis
- mice are viable and fertile (MGI Ref ID J:59307)
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Autoimmunity
Graft vs. Host Disease
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Cd4-Klra1)6295Dl | ||
|---|---|---|---|
| Allele Name | transgene insertion 6295, David Lo | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Ly-49A; Tg(Cd4-Klra1)1Dl; | ||
| Mutation Made By | Mary Pauza, Southern Illinois University | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | Klra1, killer cell lectin-like receptor, subfamily A, member 1, mouse, laboratory | ||
| Promoter | Cd4, CD4 antigen, mouse, laboratory | ||
| Molecular Note | The Ly-49A transgenic construct containing the mouse Klra1 (killer cell lectin-like receptor, subfamily A, member 1) gene under the control of a modified mouse CD4 antigen (Cd4) promoter (this promoter lacks the silencer element sequence suppressing expression in CD8 T cells) was injected into fertilized C57BL/6 mouse eggs. Founder line 6295 was established. [MGI Ref ID J:59307] | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
References
References provided by MGI
Selected Reference(s)
Pauza M; Smith KM; Neal H; Reilly C; Lanier LL; Lo D. 2000. Transgenic expression of Ly-49A in thymocytes alters repertoire selection. J Immunol 164(2):884-92. [PubMed: 10623836] [MGI Ref ID J:59307]
Additional References
Tg(Cd4-Klra1)6295Dl relatedSmith SS; Patterson T; Pauza ME. 2005. Transgenic Ly-49A inhibits antigen-driven T cell activation and delays diabetes. J Immunol 174(7):3897-905. [PubMed: 15778344] [MGI Ref ID J:97982]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. To cryorecover this strain, the oocyte donor strain (Stock No. 000999 or 001012) must also be cryorecovered to preserve the genetic background of the transgenic strain.
Purchasing information
Pricing, Supply Level & Notes, Controls
This strain is currently Awaiting Transfer from the Donor.
To register your interest in this strain go to the Strain Interest Form. View All Strains Awaiting Transfer from the Donor and On Hold
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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