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| These mice contain (STOP-hPLAP): the human alkaline phosphatase, placental (Regan isozyme), ALPP, gene under the control of the CAG (chicken beta actin promoter/enhancer and cytomegalovirus immediate-early enhancer) promoter, inserted into the Gt(ROSA)26Sor locus. Expression of the ALPP gene is blocked by a loxP-flanked STOP fragment placed between the ALPP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre-mediated excision being indicated by ALPP expression in cre-expressing tissues. | |||||||||||||||
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Description
Strain Information
Type Gene Trap; Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Fan Wang, Duke University Medical Center Description
These mice contain "STOP-hPLAP", the human alkaline phosphatase, placental (Regan isozyme), ALPP, gene under the control of the CAG (chicken beta actin promoter/enhancer and cytomegalovirus immediate-early enhancer) promoter, inserted into the Gt(ROSA)26Sor locus. Expression of the ALPP gene is blocked by a loxP-flanked STOP fragment placed between the ALPP sequence and the Gt(ROSA)26Sor promoter. In the absence of Cre recombinase, no ALPP staining is detected in all tissues examined so far including the central and peripheral nervous system, skin, muscle, lung and heart. This strain serves as a reporter strain, with successful Cre-mediated excision being indicated by ALPP expression in cre-expressing tissues. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of cell lineage tracing, and is particularly useful for labeling and tracing the morphology of neurons because ALPP is a GPI-anchored membrane protein that is transported and expressed on the membrane of axons and dendrites of neurons.Development
A targeting vector containing the human ALPP alkaline phosphatase, placental (Regan isozyme) gene driven by the CAG (chicken beta actin promoter/enhancer and cytomegalovirus immediate-early enhancer) promoter, a STOP fragment, and polyadenlyation signal sequence was inserted into the Gt(ROSA)26Sor locus. The construct was electroporated into 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for one generation before being made homozygous. Upon arrival at The Jackson Laboratory the mice were crossed with C57BL/6J once to establish the colony.
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (64 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Research Tools
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Genetics Research
Tissue/Cell Markers: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(CAG-ALPP)Fawa | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Fan Wang | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Rosa26RCAG-hPLAP; | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | The human gene driven by the CAG promoter was cloned downstream of a floxed neo cassette and 4 polyadenylation sequences. Expression of the human gene can only be achieved by cre-mediated removal of the floxed neo STOP cassette. [MGI Ref ID J:143278] [MGI Ref ID J:143859] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Que J; Wilm B; Hasegawa H; Wang F; Bader D; Hogan BL. 2008. Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development. Proc Natl Acad Sci U S A 105(43):16626-30. [PubMed: 18922767] [MGI Ref ID J:143278]
Additional References
Gt(ROSA)26Sortm1(CAG-ALPP)Fawa relatedKessler JD; Hasegawa H; Brun SN; Emmenegger BA; Yang ZJ; Dutton JW; Wang F; Wechsler-Reya RJ. 2009. N-myc alters the fate of preneoplastic cells in a mouse model of medulloblastoma. Genes Dev 23(2):157-70. [PubMed: 19171780] [MGI Ref ID J:143859]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Production.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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