Strain Name:

B6.129S-Notch2tm3Grid/J

Stock Number:

010525

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Availability:

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These floxed mutant mice possess loxP sites flanking exon 3 of the Notch2 gene, and may be useful in generating conditional mutations for studying the Notch pathway during development and embryogenesis.

Description

Strain Information

Former Names B6.129S-Notch2tm3Grid/J    (Changed: 28-APR-10 )
B6.129S1-Notch2tm3Grid/J    (Changed: 28-APR-10 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   11-DEC-12
Specieslaboratory mouse
GenerationN11+F13 (10-DEC-13)
Generation Definitions
 
Donating Investigator Tom Gridley,   Maine Medical Center Research Institute

Description
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).

When bred to a strain with early embryonic Cre recombinase expression (see Stock No. 003755 for example), this mutant mouse strain may be useful in studies of the Notch pathway during development.

When bred to a strain expressing Cre recombinase in embryos, in particular, cardiac neural crest cells (see Stock No. 005549 for example), this mutant mouse strain may be useful in studies of vascular smooth muscle development and the cardiovascular defects associated with Alagille syndrome.

When bred to a strain expressing Cre recombinase in liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of bile duct morphogenesis.

When bred to a strain expressing Cre recombinase in vascular smooth muscle cells (see Stock No. 004746 for example), this mutant mouse strain may be useful in studies of vascular smooth muscle development.

When bred to a strain expressing Cre recombinase in the olfactory epithelium and developing head structures (see Stock No. 006084 for example), this mutant mouse strain may be useful in studies of neuroglial cell maintenance.

Development
A loxP site flanked targeting vector containing a FRT-site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 3 of the targeted gene, and another loxP site was inserted downstream of exon 3. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting male chimeric animals were crossed to C57BL/6J females. The mice were bred to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice (Stock No. 003946) to remove the FRT-site flanked PGK-neo cassette. Mice that retained the loxP site flanked exon 3 were then bred to C57BL/6 mice for 10 generations and to remove the Flp recombinase allele. Heterozygotes were crossed to generate homozygotes.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

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010617   B6.129S1-Snai2tm1Grid/J
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022359   B6;129S6-Rr24tm1Axvi/Mmjax
022360   B6;129S6-Rr25tm1Axvi/Mmjax
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002788   B6;129S7-Fsttm1Zuk/J
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View Facebase: models     (61 strains)

Strains carrying other alleles of Notch2
010620   B6.129S1-Notch2tm1Grid/J
View Strains carrying other alleles of Notch2     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Alagille Syndrome 2; ALGS2   (NOTCH2)
Hajdu-Cheney Syndrome; HJCYS   (NOTCH2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Notch2tm3Grid/Notch2tm3.1Grid Tg(Alb-cre)21Mgn/0

        involves: 129S1/Sv * C57BL/6 * DBA   (conditional)
  • liver/biliary system phenotype
  • abnormal bile duct morphology
    • by P7 few bile ducts are present   (MGI Ref ID J:133171)
  • focal hepatic necrosis   (MGI Ref ID J:133171)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • blood urea nitrogen levels are normal   (MGI Ref ID J:133171)
    • increased circulating alanine transaminase level
    • increased circulating alkaline phosphatase level
    • increased circulating bilirubin level
  • growth/size/body phenotype
  • decreased body size   (MGI Ref ID J:133171)
    • decreased body weight
      • at P8 to P9, mice are 19% lighter than wild-type   (MGI Ref ID J:133171)
      • at 4 to 5 weeks of age, mice are 15% lighter than wild-type   (MGI Ref ID J:133171)
  • endocrine/exocrine gland phenotype
  • abnormal bile duct morphology
    • by P7 few bile ducts are present   (MGI Ref ID J:133171)

Notch2tm3Grid/Notch2tm3.1Grid Tg(Tagln-cre)1Her/?

        involves: 129 * C57BL/6   (conditional)
  • mortality/aging
  • partial postnatal lethality
    • there is a 50% mortality rate between birth and weaning   (MGI Ref ID J:132939)
  • cardiovascular system phenotype
  • abnormal blood flow velocity
    • mean aortic velocity in one month old mice is 1.74 m/s which is significantly faster than the mean velocity of 0.93 m/s for controls   (MGI Ref ID J:132939)
    • mean pulmonary velocity in one month old mice is 1.9 m/s which is significantly faster than the mean velocity of 1.5 m/s for controls   (MGI Ref ID J:132939)
  • aorta stenosis
    • aorta diameters of all E18.5 embryos and newborns are decreased compared to wild-type mice   (MGI Ref ID J:132939)
  • pulmonary artery stenosis
    • pulmonary arteries of E18.5 embryos and newborns are significantly smaller   (MGI Ref ID J:132939)
  • homeostasis/metabolism phenotype
  • cyanosis
    • is observed in neonates   (MGI Ref ID J:132939)

Notch2tm3Grid/Notch2tm3.1Grid Pax3tm1(cre)Joe/Pax3+

        involves: 129 * C57BL/6   (conditional)
  • mortality/aging
  • partial postnatal lethality
    • there is a 50% mortality rate between birth and weaning   (MGI Ref ID J:132939)
  • cardiovascular system phenotype
  • abnormal vascular smooth muscle morphology
    • smooth muscle cell proliferation is reduced to 9% in E16.5 embryos compared to 22.5% of wild-type embryos   (MGI Ref ID J:132939)
  • aorta stenosis
    • aorta diameters of all E18.5 embryos and newborns are decreased compared to wild-type mice   (MGI Ref ID J:132939)
    • the mean aortic diameter is 1.3 mm compared to 1.5 mm for age-matched controls   (MGI Ref ID J:132939)
  • pulmonary artery stenosis
    • pulmonary arteries of E18.5 embryos and newborns are significantly smaller   (MGI Ref ID J:132939)
  • supravalvar pulmonary trunk stenosis
    • the mean inner diameter of the pulmonary trunk is 1.22 mm compared to 1.56 mm for age-matched controls   (MGI Ref ID J:132939)
  • craniofacial phenotype
  • abnormal tooth morphology
    • dental malformations inhibit the ability of mice to feed postnatally   (MGI Ref ID J:132939)
  • growth/size/body phenotype
  • abnormal tooth morphology
    • dental malformations inhibit the ability of mice to feed postnatally   (MGI Ref ID J:132939)
  • decreased body weight
    • by one week of age, mice weigh significantly less than control littermates   (MGI Ref ID J:132939)
  • homeostasis/metabolism phenotype
  • cyanosis
    • a mild cyanotic appearance is observed in neonates   (MGI Ref ID J:132939)
  • muscle phenotype
  • abnormal vascular smooth muscle morphology
    • smooth muscle cell proliferation is reduced to 9% in E16.5 embryos compared to 22.5% of wild-type embryos   (MGI Ref ID J:132939)

Notch2tm3Grid/Notch2tm3Grid Foxg1tm1(cre)Skm/Foxg1+

        involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6   (conditional)
  • mortality/aging
  • premature death
    • nearly two-thirds do not survive to adulthood   (MGI Ref ID J:130929)
    • born in approximately Mendelian ratios (21%)   (MGI Ref ID J:130929)
  • growth/size/body phenotype
  • abnormal olfactory epithelium morphology
    • disrupted relatively uniform spacing of sustentacular nuclei   (MGI Ref ID J:130929)
    • some areas significantly disrupted and thinner, other areas appear relatively normal   (MGI Ref ID J:130929)
    • smaller and more irregularly shaped sustentacular nuclei   (MGI Ref ID J:130929)
    • gaps and a reduction in the number of dendritic tufts at the apical surface   (MGI Ref ID J:130929)
  • decreased body weight
    • no apparent weight difference at P0   (MGI Ref ID J:130929)
    • weigh 27% less than controls at 2.5 week old   (MGI Ref ID J:130929)
    • weigh 47% less than controls at 8-19 week old   (MGI Ref ID J:130929)
  • nervous system phenotype
  • abnormal nervous system physiology
    • lower Glutathione S-transferase activity in mutant olfactory epithelia   (MGI Ref ID J:130929)
    • increased neuron apoptosis
      • increased TUNEL-positive cells in the neuronal layer of the olfactory epithelium   (MGI Ref ID J:130929)
      • does not occur during early postnatal life (P0-2.5weeks), but increase as the animal ages   (MGI Ref ID J:130929)
  • abnormal sensory neuron morphology
    • disorganized olfactory sensory neurons   (MGI Ref ID J:130929)
  • increased neuronal precursor cell number
    • increased olfactory neuronal progenitors in the neuronal and apical layer in adults mutants   (MGI Ref ID J:130929)
  • neuron degeneration
    • does not occur during early postnatal life (P0-2.5weeks), but increase as the animal ages   (MGI Ref ID J:130929)
    • increased TUNEL-positive cells in the neuronal layer of the olfactory epithelium   (MGI Ref ID J:130929)
  • small pituitary gland
    • significantly smaller pituitary in size   (MGI Ref ID J:130929)
  • taste/olfaction phenotype
  • abnormal olfactory epithelium morphology
    • disrupted relatively uniform spacing of sustentacular nuclei   (MGI Ref ID J:130929)
    • some areas significantly disrupted and thinner, other areas appear relatively normal   (MGI Ref ID J:130929)
    • smaller and more irregularly shaped sustentacular nuclei   (MGI Ref ID J:130929)
    • gaps and a reduction in the number of dendritic tufts at the apical surface   (MGI Ref ID J:130929)
  • cellular phenotype
  • increased apoptosis
    • increased TUNEL-positive cells in the apical layer of the olfactory epithelium (0.3 cells/mm vs. 0/mm)   (MGI Ref ID J:130929)
    • increased neuron apoptosis
      • increased TUNEL-positive cells in the neuronal layer of the olfactory epithelium   (MGI Ref ID J:130929)
      • does not occur during early postnatal life (P0-2.5weeks), but increase as the animal ages   (MGI Ref ID J:130929)
  • increased neuronal precursor cell number
    • increased olfactory neuronal progenitors in the neuronal and apical layer in adults mutants   (MGI Ref ID J:130929)
  • endocrine/exocrine gland phenotype
  • small pituitary gland
    • significantly smaller pituitary in size   (MGI Ref ID J:130929)
  • respiratory system phenotype
  • abnormal olfactory epithelium morphology
    • disrupted relatively uniform spacing of sustentacular nuclei   (MGI Ref ID J:130929)
    • some areas significantly disrupted and thinner, other areas appear relatively normal   (MGI Ref ID J:130929)
    • smaller and more irregularly shaped sustentacular nuclei   (MGI Ref ID J:130929)
    • gaps and a reduction in the number of dendritic tufts at the apical surface   (MGI Ref ID J:130929)
  • craniofacial phenotype
  • abnormal olfactory epithelium morphology
    • disrupted relatively uniform spacing of sustentacular nuclei   (MGI Ref ID J:130929)
    • some areas significantly disrupted and thinner, other areas appear relatively normal   (MGI Ref ID J:130929)
    • smaller and more irregularly shaped sustentacular nuclei   (MGI Ref ID J:130929)
    • gaps and a reduction in the number of dendritic tufts at the apical surface   (MGI Ref ID J:130929)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Notch2tm3Grid
Allele Name targeted mutation 3, Tom Gridley
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) N2f; N2flox; Notch2Fl; Notch2flox;
Mutation Made By Tom Gridley,   Maine Medical Center Research Institute
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Notch2, notch 2
Chromosome 3
Gene Common Name(s) AGS2; AI853703; HJCYS; Motch B; N2; expressed sequence AI853703; hN2;
Molecular Note loxP sites were inserted to flank exon 3. [MGI Ref ID J:105278]

Genotyping

Genotyping Information

Genotyping Protocols

Notch2tm3Grid, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

McCright B; Lozier J; Gridley T. 2006. Generation of new Notch2 mutant alleles. Genesis 44(1):29-33. [PubMed: 16397869]  [MGI Ref ID J:105278]

Additional References

Notch2tm3Grid related

Boyle SC; Kim M; Valerius MT; McMahon AP; Kopan R. 2011. Notch pathway activation can replace the requirement for Wnt4 and Wnt9b in mesenchymal-to-epithelial transition of nephron stem cells. Development 138(19):4245-54. [PubMed: 21852398]  [MGI Ref ID J:176046]

Canalis E; Adams DJ; Boskey A; Parker K; Kranz L; Zanotti S. 2013. Notch signaling in osteocytes differentially regulates cancellous and cortical bone remodeling. J Biol Chem 288(35):25614-25. [PubMed: 23884415]  [MGI Ref ID J:203542]

Cheng HT; Kim M; Valerius MT; Surendran K; Schuster-Gossler K; Gossler A; McMahon AP; Kopan R. 2007. Notch2, but not Notch1, is required for proximal fate acquisition in the mammalian nephron. Development 134(4):801-11. [PubMed: 17229764]  [MGI Ref ID J:119907]

Germar K; Dose M; Konstantinou T; Zhang J; Wang H; Lobry C; Arnett KL; Blacklow SC; Aifantis I; Aster JC; Gounari F. 2011. T-cell factor 1 is a gatekeeper for T-cell specification in response to Notch signaling. Proc Natl Acad Sci U S A 108(50):20060-5. [PubMed: 22109558]  [MGI Ref ID J:180443]

Hashimoto-Torii K; Torii M; Sarkisian MR; Bartley CM; Shen J; Radtke F; Gridley T; Sestan N; Rakic P. 2008. Interaction between Reelin and Notch signaling regulates neuronal migration in the cerebral cortex. Neuron 60(2):273-84. [PubMed: 18957219]  [MGI Ref ID J:144065]

Hatton BA; Villavicencio EH; Pritchard J; LeBlanc M; Hansen S; Ulrich M; Ditzler S; Pullar B; Stroud MR; Olson JM. 2010. Notch signaling is not essential in sonic hedgehog-activated medulloblastoma. Oncogene 29(26):3865-72. [PubMed: 20440271]  [MGI Ref ID J:168168]

Helbig C; Gentek R; Backer RA; de Souza Y; Derks IA; Eldering E; Wagner K; Jankovic D; Gridley T; Moerland PD; Flavell RA; Amsen D. 2012. Notch controls the magnitude of T helper cell responses by promoting cellular longevity. Proc Natl Acad Sci U S A 109(23):9041-6. [PubMed: 22615412]  [MGI Ref ID J:184840]

Hunkapiller NM; Gasperowicz M; Kapidzic M; Plaks V; Maltepe E; Kitajewski J; Cross JC; Fisher SJ. 2011. A role for Notch signaling in trophoblast endovascular invasion and in the pathogenesis of pre-eclampsia. Development 138(14):2987-98. [PubMed: 21693515]  [MGI Ref ID J:173527]

Lewis KL; Caton ML; Bogunovic M; Greter M; Grajkowska LT; Ng D; Klinakis A; Charo IF; Jung S; Gommerman JL; Ivanov II; Liu K; Merad M; Reizis B. 2011. Notch2 receptor signaling controls functional differentiation of dendritic cells in the spleen and intestine. Immunity 35(5):780-91. [PubMed: 22018469]  [MGI Ref ID J:178710]

Liu Z; Chen S; Boyle S; Zhu Y; Zhang A; Piwnica-Worms DR; Ilagan MX; Kopan R. 2013. The Extracellular Domain of Notch2 Increases Its Cell-Surface Abundance and Ligand Responsiveness during Kidney Development. Dev Cell 25(6):585-98. [PubMed: 23806616]  [MGI Ref ID J:198632]

Lozier J; McCright B; Gridley T. 2008. Notch signaling regulates bile duct morphogenesis in mice. PLoS ONE 3(3):e1851. [PubMed: 18365007]  [MGI Ref ID J:133171]

Massa F; Garbay S; Bouvier R; Sugitani Y; Noda T; Gubler MC; Heidet L; Pontoglio M; Fischer E. 2013. Hepatocyte nuclear factor 1beta controls nephron tubular development. Development 140(4):886-96. [PubMed: 23362349]  [MGI Ref ID J:194050]

Morimoto M; Nishinakamura R; Saga Y; Kopan R. 2012. Different assemblies of Notch receptors coordinate the distribution of the major bronchial Clara, ciliated and neuroendocrine cells. Development 139(23):4365-73. [PubMed: 23132245]  [MGI Ref ID J:190886]

Rodriguez S; Sickles HM; Deleonardis C; Alcaraz A; Gridley T; Lin DM. 2008. Notch2 is required for maintaining sustentacular cell function in the adult mouse main olfactory epithelium. Dev Biol 314(1):40-58. [PubMed: 18155189]  [MGI Ref ID J:130929]

Sandy AR; Stoolman J; Malott K; Pongtornpipat P; Segal BM; Maillard I. 2013. Notch signaling regulates T cell accumulation and function in the central nervous system during experimental autoimmune encephalomyelitis. J Immunol 191(4):1606-13. [PubMed: 23825310]  [MGI Ref ID J:205709]

Saravanamuthu SS; Le TT; Gao CY; Cojocaru RI; Pandiyan P; Liu C; Zhang J; Zelenka PS; Brown NL. 2012. Conditional ablation of the Notch2 receptor in the ocular lens. Dev Biol 362(2):219-29. [PubMed: 22173065]  [MGI Ref ID J:180791]

Satpathy AT; Briseno CG; Lee JS; Ng D; Manieri NA; Kc W; Wu X; Thomas SR; Lee WL; Turkoz M; McDonald KG; Meredith MM; Song C; Guidos CJ; Newberry RD; Ouyang W; Murphy TL; Stappenbeck TS; Gommerman JL; Nussenzweig MC; Colonna M; Kopan R; Murphy KM. 2013. Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens. Nat Immunol 14(9):937-48. [PubMed: 23913046]  [MGI Ref ID J:208234]

Surendran K; Boyle S; Barak H; Kim M; Stomberski C; McCright B; Kopan R. 2010. The contribution of Notch1 to nephron segmentation in the developing kidney is revealed in a sensitized Notch2 background and can be augmented by reducing Mint dosage. Dev Biol 337(2):386-95. [PubMed: 19914235]  [MGI Ref ID J:157258]

Tu X; Chen J; Lim J; Karner CM; Lee SY; Heisig J; Wiese C; Surendran K; Kopan R; Gessler M; Long F. 2012. Physiological notch signaling maintains bone homeostasis via RBPjk and Hey upstream of NFATc1. PLoS Genet 8(3):e1002577. [PubMed: 22457635]  [MGI Ref ID J:183534]

Varadkar P; Kraman M; Despres D; Ma G; Lozier J; McCright B. 2008. Notch2 is required for the proliferation of cardiac neural crest-derived smooth muscle cells. Dev Dyn 237(4):1144-52. [PubMed: 18330927]  [MGI Ref ID J:132939]

Xu J; Gridley T. 2013. Notch2 is required in somatic cells for breakdown of ovarian germ-cell nests and formation of primordial follicles. BMC Biol 11:13. [PubMed: 23406467]  [MGI Ref ID J:198004]

Zanotti S; Canalis E. 2013. Notch suppresses nuclear factor of activated T cells (NFAT) transactivation and Nfatc1 expression in chondrocytes. Endocrinology 154(2):762-72. [PubMed: 23264614]  [MGI Ref ID J:194597]

Zanotti S; Canalis E. 2014. Notch1 and Notch2 expression in osteoblast precursors regulates femoral microarchitecture. Bone 62:22-8. [PubMed: 24508387]  [MGI Ref ID J:207973]

Zheng J; Watanabe H; Wines-Samuelson M; Zhao H; Gridley T; Kopan R; Shen J. 2012. Conditional deletion of Notch1 and Notch2 genes in excitatory neurons of postnatal forebrain does not cause neurodegeneration or reduction of Notch mRNAs and proteins. J Biol Chem 287(24):20356-68. [PubMed: 22505716]  [MGI Ref ID J:198896]

Zhou Y; Tanzie C; Yan Z; Chen S; Duncan M; Gaudenz K; Li H; Seidel C; Lewis B; Moran A; Libby RT; Kiernan AE; Xie T. 2013. Notch2 regulates BMP signaling and epithelial morphogenesis in the ciliary body of the mouse eye. Proc Natl Acad Sci U S A 110(22):8966-71. [PubMed: 23676271]  [MGI Ref ID J:197419]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   11-DEC-12
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Notch2tm3Grid  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Notch2tm3Grid x Homozygous for Notch2tm3Grid  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Notch2tm3Grid  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Notch2tm3Grid x Homozygous for Notch2tm3Grid  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

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Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use


General Terms and Conditions


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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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