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| These R26RDTA (or ROSA26-DTA176) mice carry a loxP-flanked stop cassette associated with an attentuated diptheria toxin. When crossed with a Cre recombinase-expressing strain, tissue/cell-specific ablation of cells can be achieved. | |||||||||||||||
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Description
Strain Information
Type Gene Trap; Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 16-FEB-11 Species laboratory mouse Generation F?+N1F3 (18-JAN-11)
Generation DefinitionsDonating Investigator Dr. Mario R Capecchi, University of Utah; HHMI Description
These R26RDTA (or ROSA26-DTA176) mice carry a loxP-flanked stop cassette associated with an attentuated diptheria toxin. When crossed with a Cre recombinase-expressing strain, tissue/cell-specific ablation of cells can be achieved. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Development
A loxP-flanked stop cassette followed by the coding sequence for an attenuated diphtheria toxin A chain (tox 176) were introduced to the Gt(ROSA)26Sor gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1-45 embryonic stem (ES) cells. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (130 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(DTA)Mrc | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Mario R Capecchi | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | R26RDTA; ROSA26-DTA176; ROSADTA; | ||
| Mutation Made By | Dr. Mario Capecchi, University of Utah; HHMI | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | The DTA176 coding sequence was targeted into the Rosa26 locus but remains unexpressed until a loxP flanked stop sequence is removed via cre-mediated recombination. [MGI Ref ID J:105927] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR
Gt(ROSA)26Sortm1(DTA)Mrc,SEPARATED MELT
Gt(ROSA)26Sortm1(DTA)Mrc, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wu S; Wu Y; Capecchi MR. 2006. Motoneurons and oligodendrocytes are sequentially generated from neural stem cells but do not appear to share common lineage-restricted progenitors in vivo. Development 133(4):581-90. [PubMed: 16407399] [MGI Ref ID J:105927]
Additional References
Gt(ROSA)26Sortm1(DTA)Mrc relatedGu X; Liu B; Wu X; Yan Y; Zhang Y; Wei Y; Pleasure SJ; Zhao C. 2011. Inducible genetic lineage tracing of cortical hem derived Cajal-Retzius cells reveals novel properties. PLoS One 6(12):e28653. [PubMed: 22174859] [MGI Ref ID J:182256]
Haldar M; Hancock JD; Coffin CM; Lessnick SL; Capecchi MR. 2007. A conditional mouse model of synovial sarcoma: insights into a myogenic origin. Cancer Cell 11(4):375-88. [PubMed: 17418413] [MGI Ref ID J:120967]
Haldar M; Karan G; Tvrdik P; Capecchi MR. 2008. Two cell lineages, myf5 and myf5-independent, participate in mouse skeletal myogenesis. Dev Cell 14(3):437-45. [PubMed: 18331721] [MGI Ref ID J:133338]
Hutcheson DA; Zhao J; Merrell A; Haldar M; Kardon G. 2009. Embryonic and fetal limb myogenic cells are derived from developmentally distinct progenitors and have different requirements for beta-catenin. Genes Dev 23(8):997-1013. [PubMed: 19346403] [MGI Ref ID J:147972]
Jackson JR; Mula J; Kirby TJ; Fry CS; Lee JD; Ubele MF; Campbell KS; McCarthy JJ; Peterson CA; Dupont-Versteegden EE. 2012. Satellite cell depletion does not inhibit adult skeletal muscle regrowth following unloading-induced atrophy. Am J Physiol Cell Physiol 303(8):C854-61. [PubMed: 22895262] [MGI Ref ID J:192777]
Jia Q; McDill BW; Sankarapandian B; Wu S; Liapis H; Holzman LB; Capecchi MR; Miner JH; Chen F. 2008. Ablation of developing podocytes disrupts cellular interactions and nephrogenesis both inside and outside the glomerulus. Am J Physiol Renal Physiol 295(6):F1790-8. [PubMed: 18842818] [MGI Ref ID J:166011]
Leslie JR; Imai F; Fukuhara K; Takegahara N; Rizvi TA; Friedel RH; Wang F; Kumanogoh A; Yoshida Y. 2011. Ectopic myelinating oligodendrocytes in the dorsal spinal cord as a consequence of altered semaphorin 6D signaling inhibit synapse formation. Development 138(18):4085-95. [PubMed: 21831918] [MGI Ref ID J:175829]
Macfarlan TS; Gifford WD; Driscoll S; Lettieri K; Rowe HM; Bonanomi D; Firth A; Singer O; Trono D; Pfaff SL. 2012. Embryonic stem cell potency fluctuates with endogenous retrovirus activity. Nature 487(7405):57-63. [PubMed: 22722858] [MGI Ref ID J:186302]
Mathew SJ; Hansen JM; Merrell AJ; Murphy MM; Lawson JA; Hutcheson DA; Hansen MS; Angus-Hill M; Kardon G. 2011. Connective tissue fibroblasts and Tcf4 regulate myogenesis. Development 138(2):371-84. [PubMed: 21177349] [MGI Ref ID J:167712]
McCarthy JJ; Mula J; Miyazaki M; Erfani R; Garrison K; Farooqui AB; Srikuea R; Lawson BA; Grimes B; Keller C; Van Zant G; Campbell KS; Esser KA; Dupont-Versteegden EE; Peterson CA. 2011. Effective fiber hypertrophy in satellite cell-depleted skeletal muscle. Development 138(17):3657-66. [PubMed: 21828094] [MGI Ref ID J:175837]
Murphy MM; Lawson JA; Mathew SJ; Hutcheson DA; Kardon G. 2011. Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration. Development 138(17):3625-37. [PubMed: 21828091] [MGI Ref ID J:174914]
Sangiorgi E; Capecchi MR. 2008. Bmi1 is expressed in vivo in intestinal stem cells. Nat Genet 40(7):915-20. [PubMed: 18536716] [MGI Ref ID J:138364]
Shen W; Fruttiger M; Zhu L; Chung SH; Barnett NL; Kirk JK; Lee S; Coorey NJ; Killingsworth M; Sherman LS; Gillies MC. 2012. Conditional Mullercell ablation causes independent neuronal and vascular pathologies in a novel transgenic model. J Neurosci 32(45):15715-27. [PubMed: 23136411] [MGI Ref ID J:192243]
Tripathi P; Guo Q; Wang Y; Coussens M; Liapis H; Jain S; Kuehn MR; Capecchi MR; Chen F. 2010. Midline signaling regulates kidney positioning but not nephrogenesis through Shh. Dev Biol 340(2):518-27. [PubMed: 20152829] [MGI Ref ID J:160256]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygotes may be bred. Mating System Homozygote x Homozygote (Female x Male) 16-FEB-11
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(DTA)Mrc
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Gt(ROSA)26Sortm1(DTA)Mrc x Homozygous for Gt(ROSA)26Sortm1(DTA)Mrc Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Homozygous for Gt(ROSA)26Sortm1(DTA)Mrc
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Gt(ROSA)26Sortm1(DTA)Mrc x Homozygous for Gt(ROSA)26Sortm1(DTA)Mrc Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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