Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Gene Trap; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse   Donating Investigator Marcia A Kaetzel,   University of Cincinnati College of Med.

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous pups on the C57BL/6 background are smaller than wildtype littermates. Homozygous males become infertile if abdominal fat accumulates. Cell-specific alternative splicing of the Anx A4 transcript can yield three distinct mRNAs (Anx A4a, b and c) that are differentially expressed yet produce protein of identical sequence. In these animals, only the widely distributed AnxA4a transcript is disrupted, eliminating protein translation from this splice form. An aberrant gene product, a fusion transcript of exon a and the neomyocin resistance gene, (mRNA) is detected by RT-PCR analysis. Expression of Anx A4b and A4c transcripts is unchanged. No gene product (protein) is detected in heart or lung, low levels are detected in kidney, liver, and testis, and high levels are in duodenum, jejunum, ileum, and colon by Western blot analysis. Homozygotes exhibit an impaired hepatic inflammatory response. This mutant mouse strain may be useful in studies of inflammation and liver cancer.

Development
A gene trap construct was electroporated into 129 derived embryonic stem (ES) cells and was found to be inserted into the first intron, downstream of exon a. Correctly targeted ES cells were injected into albino C57BL/6 blastocysts. The resulting male chimeric animals were crossed to albino C57BL/6 females. The mice were crossed to FVB/N and then backcrossed to C57BL/6 for 6 generations before being made homozygous. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J once to establish the colony.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Anxa4^{Gt(OST134786)Lex}/Anxa4^{Gt(OST134786)Lex}

        involves: 129S5/SvEvBrd * C57BL/6

• behavior/neurological phenotype
• abnormal maternal nurturing
  • females often do not care for their pups   (MGI Ref ID J:87727)
  • pups are fostered to wild-type mothers in order for them to survive   (MGI Ref ID J:87727)

Anxa4^{Gt(OST134786)Lex}/Anxa4^{Gt(OST134786)Lex}

        involves: 129S5/SvEvBrd

• renal/urinary system phenotype
• *normal* renal/urinary system phenotype
  • bladder morphology and uroepithelial ultrastructure is indistinguishable from wild-type controls   (MGI Ref ID J:133525)
  • barrier function, membrane trafficking in response to hydrostatic pressure, void volumes and intrabladder pressures are similar to controls   (MGI Ref ID J:133525)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Other

Immunology, Inflammation and Autoimmunity Research
Inflammation

Research Tools
Cancer Research
Immunology and Inflammation Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Anxa4Gt(OST134786)Lex
Allele Name		gene trap OST134786, Lexicon Genetics
Allele Type		Gene trapped
Common Name(s)		AIValpha-; Anxa4Gt(neo)418Lex;
Mutation Made By		John Dedman,   University of Cincinnati College of Med.
Strain of Origin		129S5/SvEvBrd
ES Cell Line Name		Lex-1
ES Cell Line Strain		129S5/SvEvBrd
Gene Symbol and Name		Anxa4, annexin A4
Chromosome		6
Gene Common Name(s)		AI265406; AIV; ANX4; AW106930; PIG28; Xanx-4; ZAP36; annexin IV; expressed sequence AI265406; expressed sequence AW106930;
Molecular Note		The gene locus produces three different transcript variants that express the same 35-kDa protein but differ in their upstream untranslated region (UTR). A gene trap inserted downstream of "exon a", a non-coding exon that lies 27 kb upstream from the first coding exon. This insertion disrupted the UTR for one transcript variant of the gene locus but left intact two other transcript variants. Homozygotes for this allele are hypomorphs with the only strong gene expression occurring in the intestinal tract while wild-type mice have strong gene expression in numerous tissues including heart, kidney, liver, lung, stomach, and the intestinal tract. [MGI Ref ID J:87727]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Li B; Dedman JR; Kaetzel MA. 2003. Intron disruption of the annexin IV gene reveals novel transcripts. J Biol Chem 278(44):43276-83. [PubMed: 12912993]  [MGI Ref ID J:87727]

Additional References

Anxa4^{Gt(OST134786)Lex} related

Hill WG; Meyers S; von Bodungen M; Apodaca G; Dedman JR; Kaetzel MA; Zeidel ML. 2008. Studies on localization and function of annexin A4a within urinary bladder epithelium using a mouse knockout model. Am J Physiol Renal Physiol 294(4):F919-27. [PubMed: 18256316]  [MGI Ref ID J:133525]

Zambrowicz BP; Abuin A; Ramirez-Solis R; Richter LJ; Piggott J; BeltrandelRio H; Buxton EC; Edwards J; Finch RA; Friddle CJ; Gupta A; Hansen G; Hu Y; Huang W; Jaing C; Key BW Jr; Kipp P; Kohlhauff B; Ma ZQ; Markesich D; Payne R; Potter DG; Qian N; Shaw J; Schrick J; Shi ZZ; Sparks MJ; Van Sligtenhorst I; Vogel P; Walke W; Xu N; Zhu Q; Person C; Sands AT. 2003. Wnk1 kinase deficiency lowers blood pressure in mice: a gene-trap screen to identify potential targets for therapeutic intervention. Proc Natl Acad Sci U S A 100(24):14109-14. [PubMed: 14610273]  [MGI Ref ID J:86696]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes. Homozygous pups on the C57BL/6 background are smaller than wildtype littermates. Homozygous males become infertile if abdominal fat accumulates.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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