Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?pN1 Generation Definitions   Donating Investigator Stephen Duncan,   Medical College of Wisconsin

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In response to endoplasmic reticulum (ER) stress induced by tunicamycin, homozygous mice exhibit a reduced expression of the acute phase response proteins C-reactive protein, serum amyloid P-component and serum amyloid A3. This mutant mouse strain may be useful in studies of the systemic inflammatory response to ER stress.

Development
A targeting vector was designed to place a loxP site between exons 11 and 12 and FRT-flanked neomycin resistance cassette with a loxP site between exons 3 and 4. The construct was electroporated into 129S1/SvImJ and 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to CD1 mice. To remove the floxed neo cassette and exons 4 through 11, mice were crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (Stock #003724). Mice retain a single loxP site 3 prime to exon 3. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Information

	Control
	None Available
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Creb3l3^tm1.1Sad/Creb3l3^tm1.1Sad

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

• immune system phenotype
• abnormal circulating serum amyloid protein level
  • mice fail to increase SAP levels in circulation 24 hours after injection with tunicamycin   (MGI Ref ID J:140018)
• decreased acute inflammation
  • mice fail to increase serum amyloid P-component levels after injection with tunicamycin   (MGI Ref ID J:140018)
  • mRNAs of other acute phase proteins like C-reactive protein and serum amyloid A3 also fail to increase in the liver 24 hours after tunicamycin injection   (MGI Ref ID J:140018)
• homeostasis/metabolism phenotype
• abnormal circulating serum amyloid protein level
  • mice fail to increase SAP levels in circulation 24 hours after injection with tunicamycin   (MGI Ref ID J:140018)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Inflammation

Internal/Organ Research
Liver Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Creb3l3tm1.1Sad
Allele Name		targeted mutation 1.1, Stephen A Duncan
Allele Type		Targeted (knock-out)
Common Name(s)		CrebH-;
Mutation Made By		Stephen Duncan,   Medical College of Wisconsin
Strain of Origin		129S1/Sv and 129X1/SvJ
ES Cell Line Name		Other (see notes)
ES Cell Line Strain		129
Gene Symbol and Name		Creb3l3, cAMP responsive element binding protein 3-like 3
Chromosome		10
Gene Common Name(s)		BC010786; CREB-H; CREBH; D10Bur1e; DNA segment, Chr 10, Burmeister 1, expressed; cDNA sequence BC010786;
General Note		ES cell line = Jxl1.
Molecular Note		A loxP site and a frt-flanked neomycin resistance cassette were inserted between exons 3 and 4, and a second loxP site was inserted between exons 11 and 12. The floxed region included the neo cassette. Mice were crossed with mice carrying Tg(Ella-cre)C5379Lmgd to remove exons 4 through 11 and parts of introns 3 and 11. One loxP site remains downstream of exon 3. Southern blot and PCR analyses on genomic DNA confirmed that the correct targeting and recombination events occurred. [MGI Ref ID J:140018]

Genotyping

Genotyping Information

Genotyping Protocols

Creb3l3^tm1.1Sad, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Luebke-Wheeler J; Zhang K; Battle M; Si-Tayeb K; Garrison W; Chhinder S; Li J; Kaufman RJ; Duncan SA. 2008. Hepatocyte nuclear factor 4alpha is implicated in endoplasmic reticulum stress-induced acute phase response by regulating expression of cyclic adenosine monophosphate responsive element binding protein H. Hepatology 48(4):1242-50. [PubMed: 18704925]  [MGI Ref ID J:140018]

Additional References

Creb3l3^tm1.1Sad related

Vecchi C; Montosi G; Zhang K; Lamberti I; Duncan SA; Kaufman RJ; Pietrangelo A. 2009. ER stress controls iron metabolism through induction of hepcidin. Science 325(5942):877-80. [PubMed: 19679815]  [MGI Ref ID J:151926]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	While maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	None Available
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.