Strain Name:

STOCK Creb3l3tm1.1Sad/J

Stock Number:

010538

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
This targeted mutation of cAMP responsive element binding protein 3-like 3 (Creb3l3) gene exhibits a reduction of acute phase response proteins following ER stress and may be useful in studies of the systemic inflammatory response to ER stress.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
GenerationN?pN1
Generation Definitions
 
Donating Investigator Stephen Duncan,   Medical College of Wisconsin

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In response to endoplasmic reticulum (ER) stress induced by tunicamycin, homozygous mice exhibit a reduced expression of the acute phase response proteins C-reactive protein, serum amyloid P-component and serum amyloid A3. This mutant mouse strain may be useful in studies of the systemic inflammatory response to ER stress.

Development
A targeting vector was designed to place a loxP site between exons 11 and 12 and FRT-flanked neomycin resistance cassette with a loxP site between exons 3 and 4. The construct was electroporated into 129S1/SvImJ and 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to CD1 mice. To remove the floxed neo cassette and exons 4 through 11, mice were crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (Stock #003724). Mice retain a single loxP site 3 prime to exon 3. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Creb3l3tm1.1Sad/Creb3l3tm1.1Sad

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N
  • immune system phenotype
  • abnormal circulating serum amyloid protein level
    • mice fail to increase SAP levels in circulation 24 hours after injection with tunicamycin   (MGI Ref ID J:140018)
  • decreased acute inflammation
    • mice fail to increase serum amyloid P-component levels after injection with tunicamycin   (MGI Ref ID J:140018)
    • mRNAs of other acute phase proteins like C-reactive protein and serum amyloid A3 also fail to increase in the liver 24 hours after tunicamycin injection   (MGI Ref ID J:140018)
  • homeostasis/metabolism phenotype
  • abnormal circulating serum amyloid protein level
    • mice fail to increase SAP levels in circulation 24 hours after injection with tunicamycin   (MGI Ref ID J:140018)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Inflammation

Internal/Organ Research
Liver Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Creb3l3tm1.1Sad
Allele Name targeted mutation 1.1, Stephen A Duncan
Allele Type Targeted (Null/Knockout)
Common Name(s) CrebH-;
Mutation Made By Stephen Duncan,   Medical College of Wisconsin
Strain of Origin129S1/Sv and 129X1/SvJ
ES Cell Line NameOther (see notes)
ES Cell Line Strain129
Gene Symbol and Name Creb3l3, cAMP responsive element binding protein 3-like 3
Chromosome 10
Gene Common Name(s) BC010786; CREB-H; CREBH; D10Bur1e; DNA segment, Chr 10, Burmeister 1, expressed; cDNA sequence BC010786;
General Note ES cell line = Jxl1.
Molecular Note A loxP site and a frt-flanked neomycin resistance cassette were inserted between exons 3 and 4, and a second loxP site was inserted between exons 11 and 12. The floxed region included the neo cassette. Mice were crossed with mice carrying Tg(Ella-cre)C5379Lmgd to remove exons 4 through 11 and parts of introns 3 and 11. One loxP site remains downstream of exon 3. Southern blot and PCR analyses on genomic DNA confirmed that the correct targeting and recombination events occurred. [MGI Ref ID J:140018]

Genotyping

Genotyping Information

Genotyping Protocols

Creb3l3tm1.1Sad, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Luebke-Wheeler J; Zhang K; Battle M; Si-Tayeb K; Garrison W; Chhinder S; Li J; Kaufman RJ; Duncan SA. 2008. Hepatocyte nuclear factor 4alpha is implicated in endoplasmic reticulum stress-induced acute phase response by regulating expression of cyclic adenosine monophosphate responsive element binding protein H. Hepatology 48(4):1242-50. [PubMed: 18704925]  [MGI Ref ID J:140018]

Additional References

Creb3l3tm1.1Sad related

Kim H; Mendez R; Zheng Z; Chang L; Cai J; Zhang R; Zhang K. 2014. Liver-enriched transcription factor CREBH interacts with peroxisome proliferator-activated receptor alpha to regulate metabolic hormone FGF21. Endocrinology 155(3):769-82. [PubMed: 24424044]  [MGI Ref ID J:208810]

Vecchi C; Montosi G; Zhang K; Lamberti I; Duncan SA; Kaufman RJ; Pietrangelo A. 2009. ER stress controls iron metabolism through induction of hepcidin. Science 325(5942):877-80. [PubMed: 19679815]  [MGI Ref ID J:151926]

Xu X; Park JG; So JS; Hur KY; Lee AH. 2014. Transcriptional regulation of apolipoprotein A-IV by the transcription factor CREBH. J Lipid Res 55(5):850-9. [PubMed: 24598141]  [MGI Ref ID J:210254]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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