Strain Name:

B6;129S-Fgf17tm1Dor/J

Stock Number:

010539

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Mice that are homozygous for the fibroblast growth factor 17 (Fgf17) targeted mutation exhibit impaired social interaction behavior. The vermis cerebellum and dorsal frontal cortex are reduced in size. This mutant mouse strain may be useful in studies of neuronal development, neocortical patterning and social interaction behavior.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129S6-Fgf17tm1Dor/J    (Changed: 08-JUL-09 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator David Ornitz,   Washington University School of Medicine

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. No gene product (mRNA) is detected by in situ hybridization analysis of midbrain-hindbrain from homozygous embryos. Significant portions of the inferior colliculus and the vermis cerebellum are absent in homozygotes (2 days of age and adult). The remaining midbrain and cerebellum have normal morphology although the size of the vermis cerebellum is approximately 82% of the wildtype control. The size of the rostal-most lobe of the vermis is 1/3 of the wildtype control. The fissure separating lobe III (most rostal lobe) and lobe IV does not form completely, resulting in partially fused lobes. The dorsal frontal cortex is reduced in size with a rostral shift of sensory cortical areas. Homozygotes exhibit impaired social interaction behavior. Homozygous pups vocalize less than wildtype controls when separated from mothers. Homozygous adult males display a reduction in interaction with novel ovariectomized female as part of a social recognition test and decreased social interaction in novel environments. The Donating Investigator reports that the cre recombinase gene is expressed but is not functional. This mutant mouse strain may be useful in studies of neuronal development, neocortical patterning and social interaction behavior.

Development
A targeting vector containing sequence encoding Cre recombinase, human beta actin polyadenylation sequence and a PGKneo cassette was used to disrupt exons 1a and 1b, which encode the signal peptide, and the translational start site. (The Donating Investigator reports that the cre recombinase gene is expressed but is not functional.) The construct was electroporated into 129S6/SvEvTac derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and maintained on a mixed B6;129 background. Upon arrival at The Jackson Laboratory the mice were crossed to B6129SF1/J (STOCK no. 101043) once to establish the colony.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Hypogonadotropic Hypogonadism 20 with or without Anosmia; HH20   (FGF17)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Fgf17tm1Dor/Fgf17tm1Dor

        involves: 129S6/SvEvTac
  • nervous system phenotype
  • abnormal Purkinje cell morphology
    • Purkinje cell migration begins earlier than normal   (MGI Ref ID J:61429)
  • abnormal cerebellum vermis morphology
    • overall size of vermis about 82% of control size but hemispheres normal   (MGI Ref ID J:61429)
    • decreased cell proliferation in vermis by E12.5   (MGI Ref ID J:61429)
    • abnormal cerebellum anterior vermis morphology
      • lobe III of the vermis is partially fused to lobe IV   (MGI Ref ID J:61429)
      • lobules I-III are truncated and considerably smaller in size in adult mice   (MGI Ref ID J:156717)
      • decreased anterior vermis size
        • lobe III of the vermis is about 1/3 normal size   (MGI Ref ID J:61429)
  • decreased inferior colliculus size
    • tissue losses in inferior colliculus   (MGI Ref ID J:61429)
    • the tissue between the inferior colliculus and the anterior cerebellum is significantly reduced in adult mice   (MGI Ref ID J:156717)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Behavioral and Learning Defects
Neurodevelopmental Defects

Research Tools
Developmental Biology Research
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Fgf17tm1Dor
Allele Name targeted mutation 1, David M Ornitz
Allele Type Targeted (Null/Knockout)
Common Name(s) Fgf17-; Fgf17neo;
Mutation Made By David Ornitz,   Washington University School of Medicine
Strain of Origin129S6/SvEvTac
ES Cell Line NameSM1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Fgf17, fibroblast growth factor 17
Chromosome 14
Gene Common Name(s) FGF-13; HH20;
Molecular Note A contstruct, in which the translational start site and two exons encoding the signal peptide were replaced by cre and neo genes, was inserted at the endogenous locus by homologous recombination. In situ hybridization showed a lack of transcript at the midbrain-hindbrain junction in homozygous mutant mice. Expression of Cre recombinase was not reported. [MGI Ref ID J:61429]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Xu J; Liu Z; Ornitz DM. 2000. Temporal and spatial gradients of Fgf8 and Fgf17 regulate proliferation and differentiation of midline cerebellar structures. Development 127(9):1833-43. [PubMed: 10751172]  [MGI Ref ID J:61429]

Additional References

Fgf17tm1Dor related

Basson MA; Echevarria D; Petersen Ahn C; Sudarov A; Joyner AL; Mason IJ; Martinez S; Martin GR. 2008. Specific regions within the embryonic midbrain and cerebellum require different levels of FGF signaling during development. Development 135(5):889-98. [PubMed: 18216176]  [MGI Ref ID J:131764]

Cholfin JA; Rubenstein JL. 2007. Patterning of frontal cortex subdivisions by Fgf17. Proc Natl Acad Sci U S A 104(18):7652-7. [PubMed: 17442747]  [MGI Ref ID J:121300]

Guo Q; Li K; Sunmonu NA; Li JY. 2010. Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum. Dev Biol 338(2):183-92. [PubMed: 19968985]  [MGI Ref ID J:156717]

Jen YH; Musacchio M; Lander AD. 2009. Glypican-1 controls brain size through regulation of fibroblast growth factor signaling in early neurogenesis. Neural Dev 4:33. [PubMed: 19732411]  [MGI Ref ID J:159598]

Mariani FV; Ahn CP; Martin GR. 2008. Genetic evidence that FGFs have an instructive role in limb proximal-distal patterning. Nature 453(7193):401-5. [PubMed: 18449196]  [MGI Ref ID J:135198]

Scearce-Levie K; Roberson ED; Gerstein H; Cholfin JA; Mandiyan VS; Shah NM; Rubenstein JL; Mucke L. 2008. Abnormal social behaviors in mice lacking Fgf17. Genes Brain Behav 7(3):344-54. [PubMed: 17908176]  [MGI Ref ID J:147476]

Toyoda R; Assimacopoulos S; Wilcoxon J; Taylor A; Feldman P; Suzuki-Hirano A; Shimogori T; Grove EA. 2010. FGF8 acts as a classic diffusible morphogen to pattern the neocortex. Development 137(20):3439-48. [PubMed: 20843859]  [MGI Ref ID J:165806]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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