Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129S6-Fgf17tm1Dor/J    (Changed: 08-JUL-09 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator David Ornitz,   Washington University School of Medicine

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. No gene product (mRNA) is detected by in situ hybridization analysis of midbrain-hindbrain from homozygous embryos. Significant portions of the inferior colliculus and the vermis cerebellum are absent in homozygotes (2 days of age and adult). The remaining midbrain and cerebellum have normal morphology although the size of the vermis cerebellum is approximately 82% of the wildtype control. The size of the rostal-most lobe of the vermis is 1/3 of the wildtype control. The fissure separating lobe III (most rostal lobe) and lobe IV does not form completely, resulting in partially fused lobes. The dorsal frontal cortex is reduced in size with a rostral shift of sensory cortical areas. Homozygotes exhibit impaired social interaction behavior. Homozygous pups vocalize less than wildtype controls when separated from mothers. Homozygous adult males display a reduction in interaction with novel ovariectomized female as part of a social recognition test and decreased social interaction in novel environments. The Donating Investigator reports that the cre recombinase gene is expressed but is not functional. This mutant mouse strain may be useful in studies of neuronal development, neocortical patterning and social interaction behavior.

Development
A targeting vector containing sequence encoding Cre recombinase, human beta actin polyadenylation sequence and a PGKneo cassette was used to disrupt exons 1a and 1b, which encode the signal peptide, and the translational start site. (The Donating Investigator reports that the cre recombinase gene is expressed but is not functional.) The construct was electroporated into 129S6/SvEvTac derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and maintained on a mixed B6;129 background. Upon arrival at The Jackson Laboratory the mice were crossed to B6129SF1/J (STOCK no. 101043) once to establish the colony.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Fgf17^tm1Dor/Fgf17^tm1Dor

        involves: 129S6/SvEvTac

• nervous system phenotype
• abnormal Purkinje cell morphology
  • Purkinje cell migration begins earlier than normal   (MGI Ref ID J:61429)
• abnormal cerebellum vermis morphology
  • overall size of vermis about 82% of control size but hemispheres normal   (MGI Ref ID J:61429)
  • decreased cell proliferation in vermis by E12.5   (MGI Ref ID J:61429)
• • abnormal cerebellum anterior vermis morphology
    • lobe III of the vermis is partially fused to lobe IV   (MGI Ref ID J:61429)
    • lobules I-III are truncated and considerably smaller in size in adult mice   (MGI Ref ID J:156717)
  • • decreased anterior vermis size
      • lobe III of the vermis is about 1/3 normal size   (MGI Ref ID J:61429)
• decreased inferior colliculus size
  • tissue losses in inferior colliculus   (MGI Ref ID J:61429)
  • the tissue between the inferior colliculus and the anterior cerebellum is significantly reduced in adult mice   (MGI Ref ID J:156717)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Behavioral and Learning Defects
Neurodevelopmental Defects

Research Tools
Developmental Biology Research
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Fgf17tm1Dor
Allele Name		targeted mutation 1, David M Ornitz
Allele Type		Targeted (knock-out)
Common Name(s)		Fgf17-; Fgf17neo;
Mutation Made By		David Ornitz,   Washington University School of Medicine
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		SM1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Fgf17, fibroblast growth factor 17
Chromosome		14
Gene Common Name(s)		FGF-13;
Molecular Note		A contstruct, in which the translational start site and two exons encoding the signal peptide were replaced by cre and neo genes, was inserted at the endogenous locus by homologous recombination. In situ hybridization showed a lack of transcript at the midbrain-hindbrain junction in homozygous mutant mice. Expression of Cre recombinase was not reported. [MGI Ref ID J:61429]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Xu J; Liu Z; Ornitz DM. 2000. Temporal and spatial gradients of Fgf8 and Fgf17 regulate proliferation and differentiation of midline cerebellar structures. Development 127(9):1833-43. [PubMed: 10751172]  [MGI Ref ID J:61429]

Additional References

Fgf17^tm1Dor related

Basson MA; Echevarria D; Petersen Ahn C; Sudarov A; Joyner AL; Mason IJ; Martinez S; Martin GR. 2008. Specific regions within the embryonic midbrain and cerebellum require different levels of FGF signaling during development. Development 135(5):889-98. [PubMed: 18216176]  [MGI Ref ID J:131764]

Cholfin JA; Rubenstein JL. 2007. Patterning of frontal cortex subdivisions by Fgf17. Proc Natl Acad Sci U S A 104(18):7652-7. [PubMed: 17442747]  [MGI Ref ID J:121300]

Guo Q; Li K; Sunmonu NA; Li JY. 2010. Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum. Dev Biol 338(2):183-92. [PubMed: 19968985]  [MGI Ref ID J:156717]

Jen YH; Musacchio M; Lander AD. 2009. Glypican-1 controls brain size through regulation of fibroblast growth factor signaling in early neurogenesis. Neural Dev 4:33. [PubMed: 19732411]  [MGI Ref ID J:159598]

Mariani FV; Ahn CP; Martin GR. 2008. Genetic evidence that FGFs have an instructive role in limb proximal-distal patterning. Nature 453(7193):401-5. [PubMed: 18449196]  [MGI Ref ID J:135198]

Scearce-Levie K; Roberson ED; Gerstein H; Cholfin JA; Mandiyan VS; Shah NM; Rubenstein JL; Mucke L. 2008. Abnormal social behaviors in mice lacking Fgf17. Genes Brain Behav 7(3):344-54. [PubMed: 17908176]  [MGI Ref ID J:147476]

Toyoda R; Assimacopoulos S; Wilcoxon J; Taylor A; Feldman P; Suzuki-Hirano A; Shimogori T; Grove EA. 2010. FGF8 acts as a classic diffusible morphogen to pattern the neocortex. Development 137(20):3439-48. [PubMed: 20843859]  [MGI Ref ID J:165806]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.